Analysis of human biological samples using porous graphitic carbon columns and liquid chromatography-mass spectrometry: a review.

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS Analytical and Bioanalytical Chemistry Pub Date : 2024-10-01 Epub Date: 2024-08-19 DOI:10.1007/s00216-024-05458-8

Taís Betoni Rodrigues, Ricardo Leal Cunha, Paulo Emílio Pereira Barci, Álvaro José Santos-Neto, Fernando Mauro Lanças

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Abstract

Liquid chromatography-mass spectrometry (LC-MS) has emerged as a powerful analytical technique for analyzing complex biological samples. Among various chromatographic stationary phases, porous graphitic carbon (PGC) columns have attracted significant attention due to their unique properties-such as the ability to separate both polar and non-polar compounds and their stability through all pH ranges and to high temperatures-besides the compatibility with LC-MS. This review discusses the applicability of PGC for SPE and separation in LC-MS-based analyses of human biological samples, highlighting the diverse applications of PGC-LC-MS in analyzing endogenous metabolites, pharmaceuticals, and biomarkers, such as glycans, proteins, oligosaccharides, sugar phosphates, and nucleotides. Additionally, the fundamental principles underlying PGC column chemistry and its advantages, challenges, and advances in method development are explored. This comprehensive review aims to provide researchers and practitioners with a valuable resource for understanding the capabilities and limitations of PGC columns in LC-MS-based analysis of human biological samples, thereby facilitating advancements in analytical methodologies and biomedical research.

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使用多孔石墨碳柱和液相色谱-质谱法分析人体生物样本：综述。

液相色谱-质谱联用技术（LC-MS）已成为分析复杂生物样品的强大分析技术。在各种色谱固定相中，多孔石墨化碳（PGC）色谱柱因其独特的性能而备受关注，如能分离极性和非极性化合物，在所有 pH 值范围和高温下均保持稳定，以及与 LC-MS 的兼容性。本综述讨论了 PGC 在基于 LC-MS 的人体生物样本分析中用于 SPE 和分离的适用性，重点介绍了 PGC-LC-MS 在分析内源性代谢物、药物和生物标记物（如糖、蛋白质、寡糖、糖磷酸盐和核苷酸）方面的各种应用。此外，还探讨了 PGC 色谱柱化学的基本原理及其在方法开发方面的优势、挑战和进展。本综述旨在为研究人员和从业人员提供宝贵的资源，帮助他们了解 PGC 色谱柱在基于 LC-MS 的人体生物样本分析中的能力和局限性，从而促进分析方法和生物医学研究的进步。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.