HOS15-mediated turnover of PRR7 enhances freezing tolerance

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-08-19 DOI:10.1111/nph.20062

Yeon Jeong Kim, Woe-Yeon Kim, David E. Somers

引用次数: 0

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HOS15 介导的 PRR7 转换可增强冷冻耐受性。

拟南芥 PSEUDORESPONSE REGULATOR7（PRR7）是昼夜节律振荡器的核心成分，在耐冷冻方面也起着至关重要的作用。PRR7 依靠蛋白酶体降解，在傍晚时分达到离散阶段的最大表达量。虽然它对下游基因的抑制活性是冷调节不可或缺的一部分，但 PRR7 丰度的条件调节机制尚不清楚。我们利用突变体分析、蛋白质相互作用和泛素化实验确定了泛素连接酶适配体--OSMOTICALLY RESPONSIVE GENE 15（HOS15）在低温下通过直接的蛋白质-蛋白质相互作用控制 PRR7 的蛋白质积累模式。耐冻性和电解质渗漏试验表明，PRR7 可增强对低温的敏感性，C-REPEAT BINDING FACTOR1（CBF1）和 COLD-REGULATED 15A （COR15A）启动子上的 ChIP-qPCR 也证明了这一点。在黑暗的低温条件下，HOS15 通过增强泛素化作用介导 PRR7 的转换。在相同条件下，hos15中PRR7与CBFs和COR15A启动子的关联增加与CBF1和COR15A转录减少及冷冻敏感性增强相关。我们提出了一种新的机制，即由 HOS15 介导的 PRR7 降解提供了昼夜节律系统与其他冷适应途径之间的交叉点，从而提高了冷冻耐受性。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.