Survival of Calliphora vicina (Diptera: Calliphoridae) embryos under cold temperature conditions: forensic implications.

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Medical and Veterinary Entomology Pub Date : 2024-08-19 DOI:10.1111/mve.12749
Jaime López-García, Daniel Martín-Vega
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Abstract

Most blow flies (Diptera: Calliphoridae) species are sarcosaprophagous during the larval stage, primarily feeding on the soft tissues of carcasses during the early stages of decomposition, making them valuable forensic indicators for minimum post-mortem interval (minPMI) estimations. Like other insects, their developmental rates are strongly influenced by the environmental temperature. Although several studies have examined the influence of temperature on the development of different blow fly species, the impact of cold temperatures remains largely unstudied, despite its potential forensic implications. The present study investigates the effect of three cold temperatures (0, -2.5 and -5°C) on the survival of Calliphora vicina embryos of five different ages (0%, 20%, 40%, 60% and 80% of the total embryonic development) and two exposure times (6 and 24 h). Our results revealed significant differences in egg survival at the earliest embryonic stages (0% and 20% of the total embryonic development), resulting in high mortality rates. While at 20% of the total embryonic development high mortality was only observed under -5°C, at 0% of the total embryonic development high mortality rates were observed at all the temperatures tested. Although C. vicina embryos demonstrate tolerance to cold temperatures once they have completed the first 20% of the total embryonic development, potentially mitigating the impact of cold weather events, the possibility of minPMI underestimations due to the death of the first egg batches should not be disregarded. Additionally, considering that the embryonic development stages may last for several days under low temperatures, caution should be taken in the analysis of entomological evidence if a cadaver is discovered following cold weather episodes.

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Calliphora vicina(双翅目:Calliphoridae)胚胎在低温条件下的存活:法医意义。
大多数吹蝇(双翅目:Calliphoridae)物种在幼虫阶段都是肉食性的,主要在尸体腐烂的早期阶段取食尸体的软组织,这使它们成为估算尸体最小间隔期(minPMI)的重要法医指标。与其他昆虫一样,它们的发育速度受环境温度的影响很大。尽管已有多项研究探讨了温度对不同种类吹蝇发育的影响,但低温的影响在很大程度上仍未得到研究,尽管它具有潜在的法医学意义。本研究调查了三种低温(0、-2.5 和 -5°C)对 Calliphora vicina 胚胎存活率的影响,包括五个不同的年龄段(胚胎发育的 0%、20%、40%、60% 和 80%)和两种暴露时间(6 和 24 小时)。我们的结果表明,在最早的胚胎发育阶段(胚胎发育的 0% 和 20%),卵的存活率存在显著差异,导致死亡率很高。在胚胎发育的 20% 阶段,只有在 -5°C 的条件下才能观察到高死亡率,而在胚胎发育的 0% 阶段,在所有测试温度下都能观察到高死亡率。尽管沧龙鱼胚胎在完成整个胚胎发育过程的前 20% 后表现出对低温的耐受性,有可能减轻寒冷天气事件的影响,但不应忽视由于第一批卵的死亡而导致 minPMI 被低估的可能性。此外,考虑到胚胎发育阶段在低温条件下可能会持续数天,如果在寒冷天气事件后发现尸体,则应谨慎分析昆虫学证据。
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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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