Geminivirus C4/AC4 proteins hijack cellular COAT PROTEIN COMPLEX I for chloroplast targeting and viral infections.

IF 6.5 1区生物学 Q1 PLANT SCIENCES Plant Physiology Pub Date : 2024-11-04 DOI:10.1093/plphys/kiae436

Wenhao Zhao, Yinghua Ji, Yijun Zhou, Xiaofeng Wang

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Abstract

Geminiviruses infect numerous crops and cause extensive agricultural losses worldwide. During viral infection, geminiviral C4/AC4 proteins relocate from the plasma membrane to chloroplasts, where they inhibit the production of host defense signaling molecules. However, mechanisms whereby C4/AC4 proteins are transported to chloroplasts are unknown. We report here that tomato (Solanum lycopersicum) COAT PROTEIN COMPLEX I (COPI) components play a critical role in redistributing Tomato yellow leaf curl virus C4 protein to chloroplasts via an interaction between the C4 and β subunit of COPI. Coexpression of both proteins promotes the enrichment of C4 in chloroplasts that is blocked by a COPI inhibitor. Overexpressing or downregulating gene expression of COPI components promotes or inhibits the viral infection, respectively, suggesting a proviral role of COPI components. COPI components play similar roles in C4/AC4 transport and infections of two other geminiviruses: Beet curly top virus and East African cassava mosaic virus. Our results reveal an unconventional role of COPI components in protein trafficking to chloroplasts during geminivirus infection and suggest a broad-spectrum antiviral strategy in controlling geminivirus infections in plants.

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双子叶病毒 C4/AC4 蛋白劫持了细胞 COAT PROTEIN COMPLEX I，以实现叶绿体靶向和病毒感染。

geminiviruses 可感染多种作物，并在全球范围内造成广泛的农业损失。在病毒感染过程中， geminiviral C4/AC4 蛋白会从质膜转移到叶绿体，并在叶绿体中抑制宿主防御信号分子的产生。然而，C4/AC4 蛋白迁移到叶绿体的机制尚不清楚。我们在此报告了番茄（Solanum lycopersicum）COAT PROTEIN COMPLEX I（COPI）成分通过 COPI 的 C4 和 β 亚基之间的相互作用，在将番茄黄化曲叶病毒 C4 蛋白重新分配到叶绿体中发挥了关键作用。这两种蛋白的共表达会促进叶绿体中 C4 的富集，而 COPI 抑制剂会阻止这种富集。过表达或下调 COPI 组成部分的基因表达分别会促进或抑制病毒感染，这表明 COPI 组成部分具有挑拨病毒的作用。COPI 成分在 C4/AC4 转运和其他两种 geminiviruses 的感染中发挥着类似的作用：甜菜卷曲顶病毒和东非木薯花叶病毒。我们的研究结果揭示了 COPI 组份在 geminivirus 感染过程中向叶绿体运输蛋白质过程中的非常规作用，并提出了一种控制植物中 geminivirus 感染的广谱抗病毒策略。

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来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.