The seasonal response of metabolic rate to projected climate change scenarios in aquatic amphipods

Abstract

The responses of organisms to climate change are mediated primarily by its impact on their metabolic rates, which, in turn, drive various biological and ecological processes. Although there have been numerous seminal studies on the sensitivity of metabolic rate to temperature, little is empirically known about how this rate responds to seasonal temperature ranges and beyond under conservative IPCC climate change scenarios. Here, we measured the SMR of the aquatic amphipod, Gammarus insensibilis, which served as our subject species, with body masses ranging from 0.20 to 7.74 mg ash free weight. We assessed the response of the SMR across nine temperature levels ranging from 12 to 30.2 °C. These temperatures match seasonal temperature norms, with an incremental increase of 0.6–1.2 °C above each seasonal baseline, as projected for the years 2040 and 2100 under the modest climate change scenarios. Overall, our findings showed that the effect of temperature on SMR varies with body mass, as indicated by a negative size-temperature interaction, with larger conspecifics exhibiting less sensitivity to temperature changes than smaller ones. From the cold to warm season, the SMR increased by an average of 14% °C⁻¹, with increases of 18.4% °C⁻¹ in smaller individuals and 11.4% °C⁻¹ in larger ones. The SMR of smaller individuals peaked at a 0.6 °C increase from the current summer baseline (15.08% °C⁻¹, Q10 = 4.2), while in larger ones it peaked with a 1.2 °C increase beyond autumn temperatures (14.9% °C⁻¹, Q10 = 3.9). However, at temperatures reflecting global warming that exceed summer temperatures, the SMR of larger individuals levelled off, while that of smaller ones continued to increase. Overall, our findings suggest that smaller-sized individuals have a broader thermal window for SMR performance, while the SMR of larger-sized ones will become increasingly constrained at summer temperatures as those summer temperatures become hotter.