Claudia Ravasio , Luca Teruzzi , Mirko Siano , Llorenç Cremonesi , Bruno Paroli , Marco A.C. Potenza
{"title":"A customizable digital holographic microscope","authors":"Claudia Ravasio , Luca Teruzzi , Mirko Siano , Llorenç Cremonesi , Bruno Paroli , Marco A.C. Potenza","doi":"10.1016/j.ohx.2024.e00569","DOIUrl":null,"url":null,"abstract":"<div><p>We propose a compact, portable, and low-cost holographic microscope designed for the characterization of micrometric particles suspended in a liquid. This system is built around a commercial optical microscope by substituting its illumination source (a light-emitting diode) with a collimated laser beam. Similarly, a quartz flow cell replaces the microscope glass slide using a 3D-printed custom mount. With the hardware presented in this paper, the holographic imaging of the electromagnetic fields emitted by the particles that intercept the laser beam achieves a resolution close to that of optical microscopes but with a greater depth of field. Several morphological and optical features can be extracted from the holograms, including particle projected section, aspect ratio, and extinction cross-section. Additionally, we introduce a remote system control that enables users to process the acquired holograms on a remote computational device. This work provides a comprehensive description of the methodology of image processing in holographic microscopy and a series of validation measurements conducted using calibrated particles. This technique is suitable for the characterization of airborne particles found in snow, firn, and ice; here we report experimental results obtained from Alpine ice cores.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000634/pdfft?md5=32242a7fc54507f92b0df2177c6c2c1f&pid=1-s2.0-S2468067224000634-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"HardwareX","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468067224000634","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
Abstract
We propose a compact, portable, and low-cost holographic microscope designed for the characterization of micrometric particles suspended in a liquid. This system is built around a commercial optical microscope by substituting its illumination source (a light-emitting diode) with a collimated laser beam. Similarly, a quartz flow cell replaces the microscope glass slide using a 3D-printed custom mount. With the hardware presented in this paper, the holographic imaging of the electromagnetic fields emitted by the particles that intercept the laser beam achieves a resolution close to that of optical microscopes but with a greater depth of field. Several morphological and optical features can be extracted from the holograms, including particle projected section, aspect ratio, and extinction cross-section. Additionally, we introduce a remote system control that enables users to process the acquired holograms on a remote computational device. This work provides a comprehensive description of the methodology of image processing in holographic microscopy and a series of validation measurements conducted using calibrated particles. This technique is suitable for the characterization of airborne particles found in snow, firn, and ice; here we report experimental results obtained from Alpine ice cores.
我们提出了一种结构紧凑、便于携带且成本低廉的全息显微镜,专门用于表征悬浮在液体中的微米级颗粒。该系统以商用光学显微镜为基础,用准直激光束取代其照明源(发光二极管)。同样,石英流动池使用 3D 打印的定制支架取代了显微镜玻璃载玻片。利用本文介绍的硬件,对拦截激光束的粒子发出的电磁场进行全息成像,可获得接近光学显微镜的分辨率,但景深更大。我们可以从全息图中提取多种形态和光学特征,包括粒子投影截面、长宽比和消光截面。此外,我们还引入了远程系统控制,使用户能够在远程计算设备上处理获取的全息图。这项工作全面描述了全息显微镜的图像处理方法,并使用校准粒子进行了一系列验证测量。这项技术适用于表征雪、枞树和冰中的气载颗粒;在此,我们报告了从阿尔卑斯冰芯中获得的实验结果。
HardwareXEngineering-Industrial and Manufacturing Engineering
CiteScore
4.10
自引率
18.20%
发文量
124
审稿时长
24 weeks
期刊介绍:
HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.