Overexpression of forage millet (Setaria italica) SiER genes enhances drought resistance of Arabidopsis thaliana.

IF 2.6 4区生物学 Q2 PLANT SCIENCES Functional Plant Biology Pub Date : 2024-08-01 DOI:10.1071/FP23238

Hanjing Dai, Xiaoyi Huang, Yingrun Wang, Shoujing Zhu, Jieqin Li, Zhaoshi Xu, Jiacheng Zheng

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Abstract

ERECTA (ER) is a type of receptor-like kinase that contributes a crucial mission in various aspects of plant development, physiological metabolism, and abiotic stresses responses. This study aimed to explore the functional characteristics of the SiER family genes in millet (Setaria italica L.), focusing on the growth phenotype and drought resistance of Arabidopsis overexpressed SiER4_X1 and SiER1_X4 genes (SiERs ). The results revealed that overexpression of SiER4_X1 and SiER1_X4 genes in Arabidopsis significantly enhanced the leaf number, expanded leaf length and width, further promoted the silique number, length and diameter, and plant height and main stem thickness, ultimately leading to a substantial increase in individual plant biomass. Compared to the wild-type (WT), through simulated drought stress, the expression level of SiER genes was notably upregulated, transgenic Arabidopsis seeds exhibited stronger germination rates and root development; after experiencing drought conditions, the activities of antioxidant enzymes (superoxide dismutase and peroxidase) increased, while the levels of malondialdehyde and relative electrical conductivity decreased. These results indicate that overexpression of SiERs significantly enhanced both biomass production and drought resistance in Arabidopsis . The SiER4_X1 and SiER1_X4 genes emerge as promising candidate genes for improving biomass production and drought resistance in forage plants.

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牧草小米（Setaria italica）SiER 基因的过表达可增强拟南芥的抗旱性。

ERECTA（ER）是一种受体样激酶，在植物生长发育、生理代谢和非生物胁迫响应等多方面发挥着重要作用。本研究以拟南芥过表达 SiER4_X1 和 SiER1_X4 基因（SiERs）的生长表型和抗旱性为研究对象，探讨 SiER 家族基因在小米（Setaria italica L.）中的功能特征。结果表明，拟南芥过表达SiER4_X1和SiER1_X4基因可显著增加叶片数量，扩大叶片长度和宽度，进一步促进颖果数量、长度和直径，以及植株高度和主茎粗度，最终导致单株生物量大幅增加。与野生型（WT）相比，通过模拟干旱胁迫，SiER基因的表达水平显著上调，转基因拟南芥种子表现出更强的萌发率和根系发育；经历干旱条件后，抗氧化酶（超氧化物歧化酶和过氧化物酶）的活性增加，而丙二醛和相对电导率水平下降。这些结果表明，过表达 SiERs 能显著提高拟南芥的生物量产量和抗旱性。SiER4_X1 和 SiER1_X4 基因有望成为提高饲料植物生物量生产和抗旱性的候选基因。

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来源期刊

Functional Plant Biology 生物-植物科学

CiteScore

5.50

自引率

3.30%

发文量

156

审稿时长

1 months

期刊介绍： Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance. Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science. Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.