Microbial community diversity analysis of kiwifruit pollen and identification of potential pathogens

Jiaqi Yang, Manli Yao, Dan Zhang, Yu Zhao, Guitian Gao
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Abstract

The kiwifruit industry typically uses commercial pollen for artificial pollination. However, during the collection of male flowers and pollen production, pollen can be easily contaminated by pathogenic bacteria that cause diseases such as canker and flower rot. Consequently, it is crucial to understand the structure of the pollen microbial community. This study employed Illumina high-throughput sequencing technology to analyze the fungal and bacterial composition in pollen samples from various regions in Shaanxi Province. Concurrently, potential pathogenic strains were isolated using traditional microbial isolation and cultivation techniques, and their molecular identification was performed through 16S rDNA sequence analysis. A tieback test was conducted on healthy branches to verify the pathogenicity of the strains. The results revealed a rich diversity of fungi and bacteria in kiwifruit pollen. At the phylum level, pollen fungi were mainly distributed in Ascomycota, and bacteria were mainly distributed in Proteobacteria and Firmicutes. The dominant fungal genera were Mycosphaerella, Aspergillus, and Cladosporium; the dominant bacterial genera were Weissella, Pantoea, Enterobacter, and Pseudomonas, respectively. Additionally, both Erwinia persicina and Pseudomonas fluorescens, isolated from pollen, exhibited high pathogenicity toward healthy kiwifruit branches. These findings contribute to a deeper understanding of the microbial diversity in commercial kiwifruit pollen used for mass pollination.

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猕猴桃花粉微生物群落多样性分析及潜在病原体鉴定。
猕猴桃产业通常使用商业花粉进行人工授粉。然而,在采集雄花和生产花粉的过程中,花粉很容易受到病原菌的污染,从而导致腐烂病和花腐病等疾病。因此,了解花粉微生物群落的结构至关重要。本研究采用 Illumina 高通量测序技术分析了陕西省不同地区花粉样本中的真菌和细菌组成。同时,利用传统的微生物分离和培养技术分离了潜在的病原菌株,并通过 16S rDNA 序列分析对其进行了分子鉴定。为了验证菌株的致病性,还在健康枝条上进行了回接试验。结果表明,猕猴桃花粉中的真菌和细菌具有丰富的多样性。在门的层次上,花粉真菌主要分布在子囊菌门(Ascomycota),细菌主要分布在变形菌门(Proteobacteria)和真菌门(Firmicutes)。真菌的优势菌属分别是霉菌属、曲霉属和梭菌属;细菌的优势菌属分别是魏氏菌属、泛氏菌属、肠杆菌属和假单胞菌属。此外,从花粉中分离出的Erwinia persicina和Pseudomonas fluorescens对健康的猕猴桃枝条具有很强的致病性。这些发现有助于加深对用于大规模授粉的商业猕猴桃花粉中微生物多样性的了解。
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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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