Genetic polymorphism amongst moth bean (Vigna aconitifolia (Jacq.) Marechal) accessions evaluated using various molecular markers

Abstract

This study presents genetic level diversity of 50 moth bean legume accessions (Vigna aconotifolia (Jacq.) Marechal). Despite seeds being a rich source of soluble nutritional proteins, it still remains an underutilized crop commercially. To assess the molecular diversity amongst accessions, RAPD, ISSR, DAMD, SCoT and SSR were employed as PCR markers. Of the total 5064 polymorphic bands shown with these makers, DAMD1 and SCoT1 presented higher levels of polymorphism with 407 and 262 polymorphic bands respectively. These genotypes revealed logical similarity and variation under UPGMA clustering. The PCA analysis of IC-258167, IC-415167, and IC-402286 present three highest eigen values, hence seem fit to be considered as parents for moth bean breeding programme. The polymorphic bands as produced by SCoT and DAMD primers can serve unique genotypic identifiers. This study seems to present a maiden report in labelling genetic marker based polymorphism amongst the accessions of moth bean.