Júlia Caroline Ribeiro de Carvalho , Glória Louine Vital da Costa , Gleyson Batista de Oliveira , Francisco Bruno Ferreira de Freitas , Everaldo Silvino dos Santos , Jackson Araújo de Oliveira , Carlos Eduardo de Araújo Padilha , Domingos Fabiano de Santana Souza
{"title":"Effect of flow patterns on recovery of invertases from Saccharomyces cerevisiae using polyethylene glycol/magnesium sulfate system in microchannels","authors":"Júlia Caroline Ribeiro de Carvalho , Glória Louine Vital da Costa , Gleyson Batista de Oliveira , Francisco Bruno Ferreira de Freitas , Everaldo Silvino dos Santos , Jackson Araújo de Oliveira , Carlos Eduardo de Araújo Padilha , Domingos Fabiano de Santana Souza","doi":"10.1016/j.fbp.2024.08.003","DOIUrl":null,"url":null,"abstract":"<div><p>The use of microdevices in extraction processes in aqueous two-phase systems (ATPS) has been studied for years in the literature. However, the effects of flow patterns still need to be adequately explored, especially for real samples. In this sense, the present study investigated the performance of microdevices in the recovery and partial purification of invertases with varying flow patterns. The invertases from <em>Saccharomyces cerevisiae</em> and the polyethylene glycol 1500/magnesium sulfate system were used in the experiments. Beyond the flow pattern, the study sought to maximize recovery and purification factor (PF) values by varying other operating conditions, including residence time, volumetric ratio, and channel diameter. It was observed that the short slug and long slug patterns showed better separation performance than the parallel pattern regardless of residence time and volumetric ratio. Increasing the residence time from 15 to 35 min increased the top recovery and PF values by two times, indicating that <em>S. cerevisiae</em> invertase partition is slow to the top phase. The maximum values of recovery (57.01 % ± 2.67 %) and PF (4.16 ± 0.06) were reached in the condition with long slug pattern, 35 min of operation, volumetric ratio of 2:1, and a channel diameter of 0.51 mm. The present study provides valuable information for integrating microdevices in a protocol for purifying invertase from <em>S. cerevisiae</em> and other proteins in real systems.</p></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"148 ","pages":"Pages 1-10"},"PeriodicalIF":3.5000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioproducts Processing","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960308524001512","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The use of microdevices in extraction processes in aqueous two-phase systems (ATPS) has been studied for years in the literature. However, the effects of flow patterns still need to be adequately explored, especially for real samples. In this sense, the present study investigated the performance of microdevices in the recovery and partial purification of invertases with varying flow patterns. The invertases from Saccharomyces cerevisiae and the polyethylene glycol 1500/magnesium sulfate system were used in the experiments. Beyond the flow pattern, the study sought to maximize recovery and purification factor (PF) values by varying other operating conditions, including residence time, volumetric ratio, and channel diameter. It was observed that the short slug and long slug patterns showed better separation performance than the parallel pattern regardless of residence time and volumetric ratio. Increasing the residence time from 15 to 35 min increased the top recovery and PF values by two times, indicating that S. cerevisiae invertase partition is slow to the top phase. The maximum values of recovery (57.01 % ± 2.67 %) and PF (4.16 ± 0.06) were reached in the condition with long slug pattern, 35 min of operation, volumetric ratio of 2:1, and a channel diameter of 0.51 mm. The present study provides valuable information for integrating microdevices in a protocol for purifying invertase from S. cerevisiae and other proteins in real systems.
期刊介绍:
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.