Impact of nitrapyrin on urea-based fertilizers in a Mediterranean calcareous soil: Nitrogen and microbial dynamics

Abstract

Nitrification inhibitors, such as nitrapyrin (NI), are increasingly co-applied with nitrogen (N) fertilizers as part of sustainable agricultural practice. Several studies in temperate regions have documented the effectiveness of NI in retaining soil ammonium (NH₄⁺), minimizing N loss and increasing crop yields. However, less is known about the effects of NI in Mediterranean regions, where agricultural production is challenging and requires intensive irrigation and fertilization. We investigated the short-term impact of the nitrification inhibitor nitrapyrin (2-chloro-6-(trichloromethyl)pyridine) in a two-factor mesocosm experiment, using a typical Mediterranean soil, where NI was co-applied with a selection of urea-based fertilizers: urea (U), U with urease inhibitors (U + UI), methylene urea (MU) and zeolite-coated urea (ZU). NI co-applied with urea fertilizers resulted in higher availability of soil NH₄⁺ and a concurrent increase in NH₃ volatilization. Net cumulative soil NH₄⁺ availability was 1.5–3.3 fold greater when NI was applied. Concurrently, net cumulative nitrate (NO₃⁻) and nitrite (NO₂⁻) availability was reduced by 10%–60%; this was found for all the tested fertilizer types except MU fertilizer, where the net cumulative soil NO₃⁻ and NO₂⁻ doubled. Nitrous oxide (N₂O) emissions from urea fertilization were reduced by 40% with UI, 50% with NI and 66% with NI + UI. Interestingly, after 28 d, the composition of soil microbial communities was distinctly different, due to NI application. Specifically, NI application dramatically reduced the abundance of ammonia-oxidizing and denitrifying bacterial functional groups. NI was effective in reducing N₂O emissions in this calcareous soil; however, NH₃ emissions were remarkably enhanced. These findings have important implications for the large-scale adoption of inhibitor technologies in Mediterranean agroecosystems and for the reduction of greenhouse gas emissions.