Secretion of the arthrodial membrane gland of a harvester (Arachnida: Opiliones): Antimicrobial activity

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-08-20 DOI:10.1016/j.jip.2024.108178
Norton Felipe dos Santos Silva , Rodrigo Hirata Willemart , José Roberto Machado Cunha Silva , Pedro Ismael da Silva Junior
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Abstract

Because of the exoskeleton, arthropods must have flexible areas to be able to move. Such regions are called arthrodial membranes and are particularly vulnerable to bacteria and fungi. Here, we analyzed the secretion in the glands underneath it in a Neotropical harvester Mischonyx squalidus (Arachnida: Opiliones) and tested whether it has antiseptical properties. We punctured the membrane, collected and quantified in a spectrophotometer. We also fractionated and analyzed the samples in reversed-phase high-performance liquid chromatography (RP-HPLC) and then incubated the treated fractions and determined growth inhibition by measuring absorbance. The secretions resulted in 100 fractions, among which two had activity against the Gram-positive bacteria Micrococcus luteus and against the yeast Candida albicans. The low concentrations at which the secretions were active are relevant from a biotechnological point of view. For the organism, the secretions possibly prevent infections, including when they are attacked in these regions by predators that pick that spot to bite.

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收割机(蛛形纲:Opiliones)节膜腺的分泌物:抗菌活性。
由于有外骨骼,节肢动物必须有灵活的区域才能移动。这些区域被称为节肢膜,特别容易受到细菌和真菌的侵害。在这里,我们分析了一种新热带收割机Mischonyx squalidus(蛛形纲:Opiliones)关节膜下腺体的分泌物,并测试其是否具有杀菌特性。我们刺破其薄膜,采集并用分光光度计进行量化。我们还在反相高效液相色谱法(RP-HPLC)中对样品进行了分馏和分析,然后将处理过的馏分进行培养,并通过测量吸光度来测定生长抑制作用。分泌物产生了 100 个馏分,其中有两个馏分对革兰氏阳性菌微球菌(Micrococcus luteus)和白色念珠菌(Candida albicans)具有活性。从生物技术的角度来看,分泌物的低浓度活性是有意义的。对于生物体来说,这些分泌物可能会防止感染,包括当它们在这些区域受到捕食者攻击时,捕食者会选择这个地方咬人。
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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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