Isolation and comparative analysis of culturable bacterial communities associated with life stages, breeding and rearing substrates of Culicoides oxystoma Kieffer (Diptera: Ceratopogonidae) vector of bluetongue virus

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Medical and Veterinary Entomology Pub Date : 2024-08-21 DOI:10.1111/mve.12754
Ankita Sarkar, Paramita Banerjee, Abhijit Mazumdar
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Abstract

Culicoides oxystoma Kieffer (Diptera: Ceratopogonidae) has been vectoring several arboviruses, protozoa and nematodes, leading to mortality and morbidity of livestock and wild ruminants in the tropics and subtropics. Insight into the bacterial communities associated with the vector species must be worked out. This work tries to inventorize the bacterial communities associated with this important vector species. Acquisition of gut microbiota may be the parental origin, while some are obtained through feeding during larval stages. Culicoides oxystoma possesses semi-aquatic life cycle strategies for egg-laying and larval survival. The bacteria associated with C. oxystoma were compared throughout (i) life stages: egg, larval instars, pupa, adult: male and female obtained from laboratory colony; (ii) field-collected adult: male and age-graded females; and (iii) natural breeding substrate and artificial rearing substrate. The culture-dependent bacteria were identified by Sanger sequencing of 16S rRNA, and haemolytic bacteria were screened on blood agar. Results show that Firmicutes and Proteobacteria are the predominant Phyla, of which Bacillus spp. was the most abundant across the life stages. Across the life history, Bacillus cereus, Bacillus pumilus, Bacillus tropicus, Lysinibacillus sp. and Paenibacillus sp. were retrieved routinely. Bacillus cereus and Alcaligenes faecalis were detected in the lab-reared specimens and shared between the natural breeding site and rearing medium. From the adults trapped across two locations, B. cereus, Bacillus flexus, A. faecalis, Enterococcus faecium and Pseudomonas sp. were isolated. The bacterial species associated with this vector may influence various physiological traits, such as vectorial capacity, digestion and larval development, which need further investigation.

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分离和比较分析与蓝舌病病毒病媒 Culicoides oxystoma Kieffer(Diptera: Ceratopogonidae)的生命阶段、繁殖和饲养基质相关的可培养细菌群落。
Culicoides oxystoma Kieffer(双翅目:Ceratopogonidae)一直在传播几种虫媒病毒、原生动物和线虫,导致热带和亚热带地区的家畜和野生反刍动物死亡和发病。必须深入研究与病媒物种相关的细菌群落。这项研究试图找出与这一重要病媒物种相关的细菌群落。肠道微生物群的获得可能来自亲代,也有一些是在幼虫阶段通过进食获得的。Culicoides oxystoma具有产卵和幼虫生存的半水生生命周期策略。我们比较了与氧囊虫有关的细菌在以下各阶段的分布情况:(i) 生命阶段:卵、幼虫、蛹、成虫:实验室群落中的雄虫和雌虫;(ii) 野外采集的成虫:雄虫和按年龄分级的雌虫;(iii) 天然繁殖基质和人工饲养基质。通过对 16S rRNA 进行桑格测序,鉴定了依赖培养的细菌,并在血琼脂上筛选了溶血细菌。结果表明,固着菌属和变形菌属是主要的菌门,其中芽孢杆菌属在各生命阶段的数量最多。在整个生命历程中,蜡样芽孢杆菌(Bacillus cereus)、普米氏芽孢杆菌(Bacillus pumilus)、滋养芽孢杆菌(Bacillus tropicus)、赖氨巴氏杆菌(Lysinibacillus sp.在实验室饲养的标本中检测到蜡样芽孢杆菌和粪阿卡列奈氏菌,自然繁殖地和饲养介质中也有这两种细菌。从两个地点捕获的成虫中分离出了蜡样芽孢杆菌、柔性芽孢杆菌、粪阿卡列氏菌、粪肠球菌和假单胞菌。与该病媒相关的细菌种类可能会影响各种生理特征,如病媒能力、消化和幼虫发育,这需要进一步研究。
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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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