Enhancing anti-diabetic activity and reducing cytotoxicity of T. crispa extracts through sustainable approach of pressurized hot water extraction and micelle-mediated separation

IF 3.4 3区 工程技术 Q2 CHEMISTRY, PHYSICAL Journal of Supercritical Fluids Pub Date : 2024-08-13 DOI:10.1016/j.supflu.2024.106377
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Abstract

In this study, pressurized hot water extraction (PHWE) was evaluated for the recovery of anti-diabetic borapetoside C (BPC) from T. crispa stems. The maximum BPC extraction efficiency obtained at 100 ˚C, 2.5 MPa and 5.0 mL/min was considerably higher than that obtained by the conventional methods. Under optimized conditions, one-site kinetic desorption model could most accurately describe the PHWE behavior, suggesting an intra-particle diffusion-controlled mechanism. The undesirable compounds in the extract were further removed by micelle-mediated separation (MMS), in which Tween 80 was added, followed by NaCl addition and slight temperature increase to induce phase separation. At the most suitable MMS condition, with 0.028 mM Tween 80, 0.4 M NaCl, at 85 ˚C, the majority (87 %) of BPC could be recovered in the aqueous phase after 40 min. After MMS, the resulting extract exhibited low cytotoxicity against L6 and HepG2 cells while maintaining significant α-glucosidase and α-amylase inhibition activities.

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通过加压热水萃取和胶束介导分离的可持续方法提高酥树蕨提取物的抗糖尿病活性并降低其细胞毒性
本研究评估了加压热水萃取法(PHWE)从酥树茎中回收抗糖尿病的硼磷酯 C(BPC)的效果。在 100 ˚C、2.5 MPa 和 5.0 mL/min 条件下获得的最大 BPC 提取效率大大高于传统方法。在优化条件下,单位点动力学解吸模型能最准确地描述 PHWE 的行为,表明这是一种粒子内扩散控制机制。通过胶束介导分离(MMS)进一步去除提取物中的不良化合物,在此过程中加入吐温80,然后加入NaCl并略微升温以诱导相分离。在最合适的 MMS 条件下(0.028 mM Tween 80,0.4 M NaCl,85 ˚C),40 分钟后,水相中大部分(87%)的 BPC 得以回收。经 MMS 处理后,所得提取物对 L6 和 HepG2 细胞的细胞毒性较低,但仍具有显著的 α-葡萄糖苷酶和 α-淀粉酶抑制活性。
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来源期刊
Journal of Supercritical Fluids
Journal of Supercritical Fluids 工程技术-工程:化工
CiteScore
7.60
自引率
10.30%
发文量
236
审稿时长
56 days
期刊介绍: The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.
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