Rnai-based functional analysis of bursicon genes related to cuticle pigmentation in a ladybird beetle

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY Journal of insect physiology Pub Date : 2024-08-22 DOI:10.1016/j.jinsphys.2024.104696
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Abstract

In arthropods, the binding of a bursicon (encoded by burs and pburs) heterodimer or homodimer to a leucine-rich repeat-containing G protein coupled receptor LGR2 (encoded by rk) can activate many physiological processes, especially cuticle pigmentation during insect ecdysis. In the current paper, we intended to ascertain whether bursicon signaling mediates body coloration in the 28-spotted larger potato ladybird, Henosepilachna vigintioctomaculata, and if so, by which way bursicon signal governs the pigmentation. The high expression of Hvburs, Hvpburs and Hvrk occurred in the young larvae, pupae and adults, especially in the head and ventral nerve cord. RNA interference (RNAi) aided knockdown of Hvburs, Hvpburs or Hvrk in the prepupae caused similar phenotypic defects. The pigmentation of the resultant adults was affected, with significantly reduced dark areas on the sternums. Moreover, the accumulated mRNA levels of two sclerotin biosynthesis genes, aspartate 1-decarboxylase gene Hvadc and N-β-alanyldopamine synthase gene Hvebony, were significantly increased in the Hvburs, Hvpburs or Hvrk RNAi beetles. Furthermore, depletion of either Hvadc or Hvebony could completely rescue the impaired coloration on the sternums of Hvpburs RNAi adult. Our results supported that bursicon heterodimer-mediated signal regulate cuticle pigmentation. The bursicon signaling may tune the ratio of melanins (dark/black, brown) to sclerotins (light yellow, colorless) exerting its regulative role in the pigmentation of H. vigintioctomaculata sternums.

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基于 Rnai 的瓢虫角质层色素相关囊状基因功能分析
在节肢动物中,法氏囊异源二聚体(由法氏囊和法氏囊编码)或同源二聚体与含亮氨酸丰富重复的 G 蛋白偶联受体 LGR2(由 rk 编码)的结合可激活许多生理过程,尤其是昆虫蜕皮过程中的角质层色素沉着。在本论文中,我们试图弄清法氏囊信号是否介导了 28 点大马铃薯瓢虫(Henosepilachna vigintioctomaculata)的身体着色,如果是,法氏囊信号是通过何种方式控制着色的。Hvburs、Hvpburs和Hvrk在幼虫、蛹和成虫中均有高表达,尤其是在头部和腹侧神经索。在RNAi干扰(RNAi)的帮助下敲除幼虫体内的Hvburs、Hvpburs或Hvrk会导致类似的表型缺陷。成虫的色素沉着受到影响,胸骨上的暗色区域明显减少。此外,在 Hvburs、Hvpburs 或 Hvrk RNAi 甲虫中,两个硬脂素生物合成基因(天冬氨酸 1-脱羧酶基因 Hvadc 和 N-β-丙氨酰多巴胺合成酶基因 Hvebony)的累积 mRNA 水平显著增加。此外,消耗 Hvadc 或 Hvebony 基因可以完全修复 Hvpburs RNAi 成虫胸骨上受损的着色。我们的研究结果表明,囊状体异源二聚体介导的信号调节角质层色素沉着。Bursicon 信号可能会调节黑色素(深/黑,棕色)和硬蛋白(浅黄色,无色)的比例,从而在 H. vigintioctomaculata 胸骨色素沉着中发挥调节作用。
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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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