Linc20486 promotes BmCPV replication through inhibiting the transcription of AGO2 and Dicers

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-08-21 DOI:10.1016/j.jip.2024.108170
Mengdong Wang , Weiming Tang , Chengyue Wu , Yeping Chen , Hao Li , Ping Wu , Heying Qian , Xijie Guo , Zhendong Zhang
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Abstract

The silkworm holds pivotal economic importance, serving not only as a primary source of silk but also as a prominent model organism in scientific research. Nonetheless, silkworm farming remains vulnerable to diverse factors, with viral infections posing the gravest threat to the sericulture industry. Among these, the Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), a member of the Reoviridae family and the cytoplasmic polyhedrosis virus genus, emerges as a significant pathogen in silkworm production. BmCPV infection primarily induces midgut sepsis in silkworms, spreads rapidly, and can inflict substantial economic losses on sericulture production. Presently, effective strategies for preventing and treating BmCPV infections are lacking.

Long non-coding RNA (lncRNA) constitutes a class of RNA molecules with transcripts exceeding 200 nt, playing a crucial role in mediating the interplay between pathogens and host cells. Investigation through high-throughput technology has unveiled that BmCPV infection markedly upregulates the expression of Linc20486. This observation suggests potential involvement of Linc20486 in regulating virus replication. Indeed, as anticipated, knockdown of Linc20486 in cells profoundly impedes BmCPV replication, whereas overexpression significantly enhances virus propagation.

To probe into the mechanism underlying Linc20486′s impact on virus replication, its effects on autophagy, innate immunity, and RNAi-related pathways were scrutinized. The findings revealed that Linc20486 exerts significant influence on the expression of RNAi pathway-related genes, such as Dicer1, Dicer2 and AGO2. This discovery holds promise for unveiling novel avenues to comprehend and combat BmCPV infections in silkworms.

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Linc20486 通过抑制 AGO2 和 Dicers 的转录来促进 BmCPV 的复制。
家蚕具有举足轻重的经济意义,不仅是丝绸的主要来源,也是科学研究中的重要模式生物。然而,养蚕业仍然容易受到各种因素的影响,其中病毒感染对养蚕业的威胁最大。其中,属于 Reoviridae 家族和细胞质多面体病毒属的 Bombyx mori 细胞质多面体病毒(BmCPV)成为养蚕生产中的重要病原体。BmCPV 感染主要诱发家蚕中肠败血症,传播速度快,会给养蚕生产带来巨大的经济损失。目前,还缺乏预防和治疗 BmCPV 感染的有效策略。长非编码 RNA(lncRNA)是一类转录本超过 200 nt 的 RNA 分子,在介导病原体与宿主细胞之间的相互作用方面发挥着至关重要的作用。通过高通量技术进行的研究发现,BmCPV 感染会显著上调 Linc20486 的表达。这一观察结果表明,Linc20486 可能参与了病毒复制的调控。事实上,正如预期的那样,在细胞中敲除 Linc20486 会严重阻碍 BmCPV 的复制,而过表达则会显著增强病毒的传播。为了探究Linc20486影响病毒复制的机制,研究人员仔细研究了它对自噬、先天免疫和RNAi相关途径的影响。研究结果表明,Linc20486对RNAi通路相关基因(如Dicer1、Dicer2和AGO2)的表达有显著影响。这一发现有望为理解和抗击家蚕 BmCPV 感染提供新的途径。
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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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