Pseudomonas thivervalensis K321, a promising and effective biocontrol agent for managing apple Valsa canker triggered by Valsa mali

IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pesticide Biochemistry and Physiology Pub Date : 2024-08-20 DOI:10.1016/j.pestbp.2024.106095
Yangguang Meng, Jin Li, Weiwei Yuan, Ronghao Liu, Liangsheng Xu, Lili Huang
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Abstract

Plant growth-promoting rhizobacteria (PGPR) have been reported to suppress various diseases as potential bioagents. It can inhibit disease occurrence through various means such as directly killing pathogens and inducing systemic plant resistance. In this study, a bacterium isolated from soil showed significant inhibition of Valsa mali. Morphological observations and phylogenetic analysis identified the strain as Pseudomonas thivervalensis, named K321. Plate confrontation assays demonstrated that K321 treatment severely damaged V. mali growth, with scanning electron microscopy (SEM) observations showing severe distortion of hyphae due to K321 treatment. In vitro twigs inoculation experiments indicated that K321 had good preventive and therapeutic effects against apple Valsa canker (AVC). Applying K321 on apples significantly enhanced the apple inducing systemic resistance (ISR), including induced expression of apple ISR-related genes and increased ISR-related enzyme activity. Additionally, applying K321 on apples can activate apple MAPK by enhancing the phosphorylation of MPK3 and MPK6. In addition, K321 can promote plant growth by solubilizing phosphate, producing siderophores, and producing 3-indole-acetic acid (IAA). Application of 0.2% K321 increased tomato plant height by 53.71%, while 0.1% K321 increased tomato fresh weight by 59.55%. Transcriptome analysis revealed that K321 can inhibit the growth of V. mali by disrupting the integrity of its cell membrane through inhibiting the metabolism of essential membrane components (fatty acids) and disrupting carbohydrate metabolism. In addition, transcriptome analysis also showed that K321 can enhance plant resistance to AVC by inducing ISR-related hormones and MAPK signaling, and application of K321 significantly induced the transcription of plant growth-related genes. In summary, an excellent biocontrol strain has been discovered that can prevent AVC by inducing apple ISR and directly killing V. mali. This study indicated the great potential of P. thivervalensis K321 for use as a biological agent for the control of AVC.

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假单胞菌(Pseudomonas thivervalensis K321),一种有希望且有效的生物防治剂,可用于防治由苹果蠹蛾(Valsa mali)引发的苹果蠹蛾腐烂病
据报道,植物生长促进根瘤菌(PGPR)作为潜在的生物制剂,可抑制多种病害。它可以通过直接杀死病原体和诱导植物系统抗性等多种途径抑制病害的发生。在本研究中,从土壤中分离出的一种细菌对 Valsa mali 有明显的抑制作用。通过形态学观察和系统发育分析,确定该菌株为假单胞菌(Pseudomonas thivervalensis),命名为 K321。平板对抗试验表明,K321 处理严重破坏了 V. mali 的生长,扫描电子显微镜(SEM)观察显示,K321 处理导致菌丝严重扭曲。体外树枝接种实验表明,K321 对苹果瓦尔萨腐烂病(AVC)具有良好的预防和治疗效果。在苹果上施用 K321 能显著增强苹果的诱导系统抗性(ISR),包括诱导苹果 ISR 相关基因的表达和提高 ISR 相关酶的活性。此外,在苹果上施用 K321 还能通过增强 MPK3 和 MPK6 的磷酸化激活苹果 MAPK。此外,K321 还能通过溶解磷酸盐、产生苷元和 3-吲哚乙酸(IAA)来促进植物生长。施用 0.2% 的 K321 可使番茄株高增加 53.71%,0.1% 的 K321 可使番茄鲜重增加 59.55%。转录组分析表明,K321 能通过抑制必需膜成分(脂肪酸)的代谢和破坏碳水化合物的代谢,破坏其细胞膜的完整性,从而抑制马利叶蝉的生长。此外,转录组分析还表明,K321 能通过诱导 ISR 相关激素和 MAPK 信号转导增强植物对 AVC 的抗性,施用 K321 能显著诱导植物生长相关基因的转录。总之,通过诱导苹果 ISR 和直接杀死苹果蠹蛾,发现了一种能预防苹果蠹蛾的优良生物防治菌株。这项研究表明,P. thivervalensis K321 作为一种生物制剂用于防治苹果蠹蛾的潜力巨大。
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来源期刊
CiteScore
7.00
自引率
8.50%
发文量
238
审稿时长
4.2 months
期刊介绍: Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.
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