A serosurvey examining exposure to Borrelia burgdorferi sensu lato and tick-borne encephalitis virus in Danish blood donors, August 2022

IF 1.5 Q4 INFECTIOUS DISEASES IJID regions Pub Date : 2024-08-06 DOI:10.1016/j.ijregi.2024.100414

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Abstract

Objectives

Borrelia burgdorferi sensu lato (Bbsl) and tick-borne encephalitis virus (TBEV) are tick-borne pathogens. This study aimed to investigate the seroprevalence of these pathogens in Danish blood donors.

Methods

A total of 1000 plasma samples equally distributed (n = 200) from all five Danish regions were analyzed. Commercially available enzyme-linked immunosorbent assays were used to screen the samples for immunoglobulin G antibodies against Bbsl and TBEV. The samples positive for antibodies against TBEV were further examined with a commercially available enzyme-linked immunosorbent assay and a Luminex-based TBEV suspension multiplex immunoassay for specific antibodies against non-structural protein 1 (NS1) antigen suggestive of previous infection.

Results

A total of 62 samples tested positive for immunoglobulin G antibodies against Bbsl. A total of 40 samples were positive or borderline for antibodies against TBEV, indicating potential infection or vaccination. Of these, one had antibodies against NS1, indicating past infection. The seroprevalence of Bbsl was 6.2% (95% confidence interval 4.8-7.8), with equal seroprevalence in all five regions. The seroprevalence of TBEV was 0.1% (95% confidence interval 0.01-0.62%).

Conclusions

The seroprevalence of Bbsl was similar throughout the country and corresponds well with previous studies. The seroprevalence of TBEV NS1 was low, which is in line with a low number of reported tick-borne encephalitis cases in Denmark. The NS1 positive sample was from the Capital Region, an endemic TBEV area.

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2022 年 8 月，对丹麦献血者进行血清调查，检查他们是否暴露于伯多弗氏包柔氏菌和蜱传脑炎病毒。

目的蜱传脑炎病毒（TBEV）和普通勃氏包虫病（Bbsl）是蜱传病原体。本研究旨在调查这些病原体在丹麦献血者中的血清流行率。方法分析了来自丹麦所有五个地区的 1000 份血浆样本（n = 200），这些样本平均分配。使用市售的酶联免疫吸附测定法筛查样本中针对 Bbsl 和 TBEV 的免疫球蛋白 G 抗体。对 TBEV 抗体呈阳性的样本进一步使用市售的酶联免疫吸附测定法和基于 Luminex 的 TBEV 悬浮液多重免疫测定法进行检测，以检测提示既往感染的非结构蛋白 1（NS1）抗原的特异性抗体。共有 40 份样本的 TBEV 抗体呈阳性或边缘阳性，表明可能感染或接种过疫苗。其中，1 份样本的 NS1 抗体阳性，表明曾感染过。Bbsl 的血清流行率为 6.2%（95% 置信区间为 4.8-7.8），五个地区的血清流行率相同。结论全国的 Bbsl 血清流行率相似，与之前的研究结果一致。TBEV NS1的血清流行率较低，这与丹麦报告的蜱传脑炎病例较少的情况相符。NS1 阳性样本来自 TBEV 流行区首都地区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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