N-acetylcysteine stimulates the proliferation and differentiation in heat-stressed skeletal muscle cells

IF 2.9 2区生物学 Q2 BIOLOGY Journal of thermal biology Pub Date : 2024-08-01 DOI:10.1016/j.jtherbio.2024.103958

引用次数: 0

Abstract

N-acetylcysteine (NAC) is known for its beneficial effects on health due to its antioxidant and antiapoptotic properties. This study explored the protective effects of NAC against oxidative stress in heat-stressed (HS) skeletal muscle cells and its role in promoting muscle development. NAC reduced the heat shock response by decreasing the expression of heat shock protein 70 (HSP70) in HS-induced muscle cells during proliferation and differentiation. NAC also mitigated HS-induced oxidative stress via increasing the antioxidant enzyme levels and reducing oxidant enzyme levels. Treatment with NAC at 2 mM increased cell viability from 43.68% ± 5.14%–66.69% ± 14.43% and decreased the apoptosis rate from 7.89% ± 0.53%–5.17% ± 0.11% in skeletal muscle cells. Additionally, NAC promoted the proliferation and differentiation of HS-induced skeletal muscle cells by upregulating the expression of PAX7, MYF5, MRF4 and MYHC. These findings suggest that NAC alleviates HS-induced oxidative damage in skeletal muscle cells and support muscle development.

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N- 乙酰半胱氨酸可刺激热应激骨骼肌细胞的增殖和分化。

众所周知，N-乙酰半胱氨酸（NAC）具有抗氧化和抗细胞凋亡的特性，对健康有益。本研究探讨了 NAC 对热应激（HS）骨骼肌细胞氧化应激的保护作用及其在促进肌肉发育方面的作用。在 HS 诱导的肌肉细胞增殖和分化过程中，NAC 通过降低热休克蛋白 70（HSP70）的表达来减少热休克反应。NAC 还能通过提高抗氧化酶水平和降低氧化酶水平来减轻 HS 诱导的氧化应激。用 2 mM 的 NAC 处理骨骼肌细胞，细胞存活率从 43.68% ± 5.14%-66.69% ± 14.43%，凋亡率从 7.89% ± 0.53%-5.17% ± 0.11%。此外，NAC 还能通过上调 PAX7、MYF5、MRF4 和 MYHC 的表达，促进 HS 诱导的骨骼肌细胞的增殖和分化。这些研究结果表明，NAC 可减轻 HS 诱导的骨骼肌细胞氧化损伤，支持肌肉发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of thermal biology 生物-动物学

CiteScore

5.30

自引率

7.40%

发文量

196

审稿时长

14.5 weeks

期刊介绍： The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles