Sex Differences in the Absorption, Disposition, Metabolism, and Excretion of Grape Seed Proanthocyanidins in Prepubescent Rats

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Molecular Nutrition & Food Research Pub Date : 2024-08-28 DOI:10.1002/mnfr.202400399

Lisard Iglesias-Carres, Anna Mas-Capdevila, Francisca I. Bravo, Manuel Suárez, Anna Arola-Arnal, Begoña Muguerza

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Abstract

Scope

The absorption, disposition, metabolism, and excretion (ADME) of phenolic compounds are key factors in determining their bioactivity. The group demonstrates that the ADME of a Grape Seed Proanthocyanidin Extract (GSPE) depends on sex in adult rats and specifically, methylated metabolites are only quantified in brain male adult rats. The aim of this study is to determine whether these differences exist before puberty.

Methods and results

Prepubescent 4-week-old male and female Wistar rats are administered GSPE at a dose of 1000 mg kg⁻¹. Plasma, liver, mesenteric white adipose tissue (MWAT), brain, and kidneys are extracted excised 2 h after GSPE administration, and the PAs metabolite profile is studied by HPLC-ESI-MS/MS. Moreover, plasma estradiol and brain and liver catechol-O-methyltransferase (COMT) protein levels are also studied. Results showed that there are no differences in plasma and brain among sexes and only differences are observed in liver, MWAT, and kidney with individual metabolites. This agrees with the lack of differences in estradiol and COMT levels among sexes. However, the ADME of PAs metabolites is higher in male rats.

Conclusions

The results demonstrate lack of sex-dependence in metabolite profile in prepubescent rats, suggesting that sex differences in the metabolism of GSPE occur due to puberty.

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青春期前大鼠对葡萄籽原花青素的吸收、分配、代谢和排泄的性别差异

范围：酚类化合物的吸收、处置、代谢和排泄（ADME）是决定其生物活性的关键因素。研究小组证明，葡萄籽原花青素提取物（GSPE）的ADME取决于成年大鼠的性别，特别是甲基化代谢物只在脑部雄性成年大鼠中定量。本研究的目的是确定这些差异是否存在于青春期之前：给青春期前 4 周大的雄性和雌性 Wistar 大鼠注射剂量为 1000 毫克/千克的 GSPE。给药 2 小时后，切除血浆、肝脏、肠系膜白色脂肪组织（MWAT）、大脑和肾脏，采用 HPLC-ESI-MS/MS 方法研究 PAs 代谢物谱。此外，还研究了血浆雌二醇、脑和肝儿茶酚-O-甲基转移酶（COMT）蛋白水平。结果表明，血浆和大脑中的雌二醇在性别上没有差异，只有肝脏、MWAT 和肾脏中的单个代谢物存在差异。这与雌二醇和 COMT 水平在性别间无差异的结论一致。然而，雄性大鼠的 PAs 代谢物 ADME 较高：结论：研究结果表明，青春期前大鼠的代谢物谱缺乏性别依赖性，这表明 GSPE 的代谢因青春期而出现性别差异。

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来源期刊

Molecular Nutrition & Food Research 工程技术-食品科技

CiteScore

8.70

自引率

1.90%

发文量

250

审稿时长

1.7 months

期刊介绍： Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.