Laura Bravo, Sara Martínez-López, Jose Luis Sierra-Cinos, Raquel Mateos, Beatriz Sarriá
Yerba mate has been reported to have antihypertensive, hypocholesterolemic, antidiabetic, or antiobesity properties. Most evidences from human trials involved intakes of high amounts of mate by habitual consumers. Considering its increasing popularity, this study aimed at assessing the potential cardiometabolic effects of moderate intake of yerba mate by nonhabitual consumers. A randomized, crossover, controlled study was carried out in healthy and hypercholesterolemic subjects. Anthropometric parameters, blood pressure, blood lipids, glucose metabolism, inflammatory cytokines, chemokines, and different markers of endothelial function, as well as incretins, adipocytokines, and different hormones were measured at baseline and after 8 weeks consuming yerba mate or a decaffeinated isotonic drink (control). After daily consumption of three servings of mate tea, blood pressure, inflammatory cytokines, chemokines, and colony-stimulating factors decreased in all participants. LDL-C decreased in normocholesterolemic individuals, while the mate and control interventions elicited similar hypolipidemic action in the hypercholesterolemic group. Ghrelin and glucose-dependent insulinotropic polypeptide (GIP) significantly decreased after mate intake, while glucagon-like peptide 1 (GLP-1) and adipocytokines remained unchanged. Body fat percentage and tricipital skinfold decreased only in healthy subjects, with no effects on total body weight. In conclusion, yerba mate could exert cardiometabolic protective effects in healthy consumers and in subjects at moderate cardiovascular risk.
{"title":"Yerba Mate (Ilex paraguariensis St. Hill.) Tea May Have Cardiometabolic Beneficial Effects in Healthy and At-Risk Subjects: A Randomized, Controlled, Blind, Crossover Trial in Nonhabitual Consumers","authors":"Laura Bravo, Sara Martínez-López, Jose Luis Sierra-Cinos, Raquel Mateos, Beatriz Sarriá","doi":"10.1002/mnfr.70065","DOIUrl":"https://doi.org/10.1002/mnfr.70065","url":null,"abstract":"Yerba mate has been reported to have antihypertensive, hypocholesterolemic, antidiabetic, or antiobesity properties. Most evidences from human trials involved intakes of high amounts of mate by habitual consumers. Considering its increasing popularity, this study aimed at assessing the potential cardiometabolic effects of moderate intake of yerba mate by nonhabitual consumers. A randomized, crossover, controlled study was carried out in healthy and hypercholesterolemic subjects. Anthropometric parameters, blood pressure, blood lipids, glucose metabolism, inflammatory cytokines, chemokines, and different markers of endothelial function, as well as incretins, adipocytokines, and different hormones were measured at baseline and after 8 weeks consuming yerba mate or a decaffeinated isotonic drink (control). After daily consumption of three servings of mate tea, blood pressure, inflammatory cytokines, chemokines, and colony-stimulating factors decreased in all participants. LDL-C decreased in normocholesterolemic individuals, while the mate and control interventions elicited similar hypolipidemic action in the hypercholesterolemic group. Ghrelin and glucose-dependent insulinotropic polypeptide (GIP) significantly decreased after mate intake, while glucagon-like peptide 1 (GLP-1) and adipocytokines remained unchanged. Body fat percentage and tricipital skinfold decreased only in healthy subjects, with no effects on total body weight. In conclusion, yerba mate could exert cardiometabolic protective effects in healthy consumers and in subjects at moderate cardiovascular risk.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"39 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raquel López-Gálvez, Esteban Orenes-Piñero, José Miguel Rivera-Caravaca, Fernando Pérez-Sanz, María Pilar Ramos-Bratos, Marta Isabel Roca, Darío Mandaglio-Collados, Cecilia López-García, Pablo Gil-Pérez, María Asunción Esteve-Pastor, Francisco Marín
Acute coronary syndrome (ACS) is a leading cause of global mortality, largely due to atherosclerosis influenced by lifestyle factors like diet. Gut microbiota impacts lipid metabolism, inflammation, and endothelial function, all vital in atherosclerosis. Dysbiosis increases intestinal permeability, causing inflammation and plaque instability, elevating cardiac event risk. This study investigates the impact of dietary improvements on gut microbiota and metabolite release in recent ACS patients versus healthy individuals. A cohort of 29 recent ACS patients receiving lipid-lowering therapy and dietary advice was analyzed alongside 56 healthy controls. Dietary habits, serum, and stool samples were collected at admission and after 3 months. Metagenomic analysis of stool and metabolomic analysis of serum were conducted. The results showed bacterial dysbiosis in ACS patients, characterized by a reduction in beneficial genera and an increase in potentially pro-inflammatory bacteria. After 3 months of dietary improvements, three metabolites with anti-inflammatory properties were significantly upregulated. The findings highlight the association between gut microbiota dysbiosis, fatty diets, and inflammation in ACS patients. The observed increase in anti-inflammatory metabolites following dietary changes underscore the following dietary interventions in modulating gut microbiota and improving cardiovascular and metabolic health.
{"title":"Microbial Insights: The Role of Diet in Modulating Gut Microbiota and Metabolites After Acute Coronary Syndrome","authors":"Raquel López-Gálvez, Esteban Orenes-Piñero, José Miguel Rivera-Caravaca, Fernando Pérez-Sanz, María Pilar Ramos-Bratos, Marta Isabel Roca, Darío Mandaglio-Collados, Cecilia López-García, Pablo Gil-Pérez, María Asunción Esteve-Pastor, Francisco Marín","doi":"10.1002/mnfr.70046","DOIUrl":"https://doi.org/10.1002/mnfr.70046","url":null,"abstract":"Acute coronary syndrome (ACS) is a leading cause of global mortality, largely due to atherosclerosis influenced by lifestyle factors like diet. Gut microbiota impacts lipid metabolism, inflammation, and endothelial function, all vital in atherosclerosis. Dysbiosis increases intestinal permeability, causing inflammation and plaque instability, elevating cardiac event risk. This study investigates the impact of dietary improvements on gut microbiota and metabolite release in recent ACS patients versus healthy individuals. A cohort of 29 recent ACS patients receiving lipid-lowering therapy and dietary advice was analyzed alongside 56 healthy controls. Dietary habits, serum, and stool samples were collected at admission and after 3 months. Metagenomic analysis of stool and metabolomic analysis of serum were conducted. The results showed bacterial dysbiosis in ACS patients, characterized by a reduction in beneficial genera and an increase in potentially pro-inflammatory bacteria. After 3 months of dietary improvements, three metabolites with anti-inflammatory properties were significantly upregulated. The findings highlight the association between gut microbiota dysbiosis, fatty diets, and inflammation in ACS patients. The observed increase in anti-inflammatory metabolites following dietary changes underscore the following dietary interventions in modulating gut microbiota and improving cardiovascular and metabolic health.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"29 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We aimed to study the role of Apolipoprotein B (Apo-B) polymorphisms (Ins/Del and EcoRI) and genotype interaction on lipid profiles and atherogenic indices in response to changes in dietary total antioxidant capacity (DTAC) of diet. This cross-sectional study consisted of 700 diabetic patients. Biochemical markers including total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), superoxide dismutase (SOD), C-reactive protein (CRP), total antioxidant capacity (TAC), interlukin-18 (IL-18), and Prostaglandin F2α (PGF2α) were measured based on standard protocols. Genotyping of the Apo-B polymorphisms was conducted by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Subjects with Ins/Ins genotype with higher DTAC intake had lower TG, AIP, and AC compared to Del-allele carriers. Moreover, A-allele carriers (EcoRI) with a higher median intake of DTAC had lower body mass index (BMI) and waist circumference (WC) compared to GG homozygotes. For combined genotypes, the EcoRI only variant (Ins/Ins and AA + AG) with higher DTAC intake had lower BMI and WC. Moreover, Ins/Del only variant (Ins/del + del/del and GG) with more adherence to DTAC had higher TG and AIP. Our study showed that Apo-B polymorphisms interact with the antioxidant capacity of diet to ameliorate the risk of cardio-metabolic diseases, especially atherosclerosis in the A carriers of EcoR1 and Ins/Ins homozygous of Ins/Del polymorphism.
{"title":"Dietary Total Antioxidant Capacity Can Modify the Effects of Apo-B Polymorphisms (Ins/Del and EcoRI) on Lipid Profiles and Atherogenic Indices Between Diabetic Patients","authors":"Faezeh Abaj, Masoumeh Rafiee, Ronak Nikbazm, Ehsan Alvandi, Fariba Koohdani","doi":"10.1002/mnfr.70009","DOIUrl":"https://doi.org/10.1002/mnfr.70009","url":null,"abstract":"We aimed to study the role of Apolipoprotein B (Apo-B) polymorphisms (Ins/Del and EcoRI) and genotype interaction on lipid profiles and atherogenic indices in response to changes in dietary total antioxidant capacity (DTAC) of diet. This cross-sectional study consisted of 700 diabetic patients. Biochemical markers including total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), superoxide dismutase (SOD), C-reactive protein (CRP), total antioxidant capacity (TAC), interlukin-18 (IL-18), and Prostaglandin F2α (PGF2α) were measured based on standard protocols. Genotyping of the Apo-B polymorphisms was conducted by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Subjects with Ins/Ins genotype with higher DTAC intake had lower TG, AIP, and AC compared to Del-allele carriers. Moreover, A-allele carriers (EcoRI) with a higher median intake of DTAC had lower body mass index (BMI) and waist circumference (WC) compared to GG homozygotes. For combined genotypes, the EcoRI only variant (Ins/Ins and AA + AG) with higher DTAC intake had lower BMI and WC. Moreover, Ins/Del only variant (Ins/del + del/del and GG) with more adherence to DTAC had higher TG and AIP. Our study showed that Apo-B polymorphisms interact with the antioxidant capacity of diet to ameliorate the risk of cardio-metabolic diseases, especially atherosclerosis in the A carriers of EcoR1 and Ins/Ins homozygous of Ins/Del polymorphism.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"385 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ilaria Minato, Pedro Mena, Luigi Ricciardiello, Eleonora Scaioli, Andrea Belluzzi, Enrica Rotondo, Eleonora Derlindati, Barbara Montanini, Costanza Michelini, Nicole Tosi, Vicente Agullò Garcià, Gianfranco Picone, Carlo Mengucci, Sara Dobani, Paloma Salamanca, Alice Rosi, Margherita Dall'Asta, Letizia Bresciani, Claudio Curti, Enzo Spisni, Alessandra Dei Cas, Alessandra Bordoni, Francisco A. Tomás‐Barberán, Lynnette R. Ferguson, Daniele Del Rio, Francesca Danesi
This study aimed at investigating the effects of pomegranate juice (POMJ) consumption on inflammatory biomarkers and gene expression in patients with inflammatory bowel disease (IBD) in clinical remission. In this randomized, placebo‐controlled trial, 16 subjects with IBD in remission consumed POMJ or placebo for 12 weeks. POMJ consumption significantly reduced fecal calprotectin (FC) and plasma endotoxin levels. Transcriptomic analysis of peripheral blood mononuclear cells revealed upregulation of genes involved in mucosal immunity, including aryl hydrocarbon receptor (AHR), neutrophil cytosolic factor 4 (NCF4), and nuclear factor, interleukin 3 regulated (NFIL3). Urolithin metabotypes were predominantly of the B type, associated with intestinal dysbiosis. No significant changes were observed in serum inflammatory markers or colonic mucosal cytokine expression. POMJ consumption reduced markers of intestinal inflammation and modulated gene expression related to mucosal immunity and barrier function in patients with IBD. These findings suggest the potential of POMJ as a beneficial dietary intervention for maintaining remission in IBD, highlighting the promise of targeted nutritional strategies in managing chronic inflammatory conditions. Further research is needed to elucidate the long‐term clinical implications of these molecular changes.Trial RegistrationClinicalTrials.gov identifier: NCT03000101.
{"title":"Evidence for a Modulatory Effect of a 12‐Week Pomegranate Juice Intervention on the Transcriptional Response in Inflammatory Bowel Disease Patients Reducing Fecal Calprotectin Levels: Findings From a Proof‐of‐Principle Study","authors":"Ilaria Minato, Pedro Mena, Luigi Ricciardiello, Eleonora Scaioli, Andrea Belluzzi, Enrica Rotondo, Eleonora Derlindati, Barbara Montanini, Costanza Michelini, Nicole Tosi, Vicente Agullò Garcià, Gianfranco Picone, Carlo Mengucci, Sara Dobani, Paloma Salamanca, Alice Rosi, Margherita Dall'Asta, Letizia Bresciani, Claudio Curti, Enzo Spisni, Alessandra Dei Cas, Alessandra Bordoni, Francisco A. Tomás‐Barberán, Lynnette R. Ferguson, Daniele Del Rio, Francesca Danesi","doi":"10.1002/mnfr.70067","DOIUrl":"https://doi.org/10.1002/mnfr.70067","url":null,"abstract":"<jats:label/>This study aimed at investigating the effects of pomegranate juice (POMJ) consumption on inflammatory biomarkers and gene expression in patients with inflammatory bowel disease (IBD) in clinical remission. In this randomized, placebo‐controlled trial, 16 subjects with IBD in remission consumed POMJ or placebo for 12 weeks. POMJ consumption significantly reduced fecal calprotectin (FC) and plasma endotoxin levels. Transcriptomic analysis of peripheral blood mononuclear cells revealed upregulation of genes involved in mucosal immunity, including aryl hydrocarbon receptor (<jats:italic>AHR</jats:italic>), neutrophil cytosolic factor 4 (<jats:italic>NCF4</jats:italic>), and nuclear factor, interleukin 3 regulated (<jats:italic>NFIL3</jats:italic>). Urolithin metabotypes were predominantly of the B type, associated with intestinal dysbiosis. No significant changes were observed in serum inflammatory markers or colonic mucosal cytokine expression. POMJ consumption reduced markers of intestinal inflammation and modulated gene expression related to mucosal immunity and barrier function in patients with IBD. These findings suggest the potential of POMJ as a beneficial dietary intervention for maintaining remission in IBD, highlighting the promise of targeted nutritional strategies in managing chronic inflammatory conditions. Further research is needed to elucidate the long‐term clinical implications of these molecular changes.Trial RegistrationClinicalTrials.gov identifier: <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"https://clinicaltrials.gov/ct2/show/NCT03000101\">NCT03000101</jats:ext-link>.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"13 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marco Rendine, Samuele Venturi, Mirko Marino, Claudio Gardana, Peter Møller, Daniela Martini, Patrizia Riso, Cristian Del Bo
The aim of the study was to assess the effects of quercetin metabolites (QMs) on lipid accumulation in adipocytes under high-glucose and physiological-glucose concentrations and to elucidate the mechanisms involved. 3T3-L1 mature adipocytes were exposed to a physiological glucose concentration, as a model of caloric restriction (CR), or high glucose (control), with and without QMs (quercetin-3-glucuronide [Q3G] and isorhamnetin [ISOR]). Cells were treated with Q3G (0.3 and 0.6 µmol/L) and ISOR (0.2 and 0.4 µmol/L) for 48 h. Lipid accumulation (Oil Red O staining) and Δ glucose level (HPLC) were assessed. Under high glucose, Q3G and ISOR reduced lipid accumulation (−10.8% and −10.4%; p < 0.01) and Δ glucose level (−13.6% and −14.2%; p < 0.05). Under CR, QMs increased Δ glucose level (+21.6% for Q3G and +21% for ISOR; p < 0.05). ISOR increased pAMPK levels under high glucose (+1.4-fold; p < 0.05). Under CR, Q3G and ISOR increased pAMPK (+1.4- and +1.5-fold; p < 0.05), while ISOR upregulated SIRT1 and PGC-1α (+2.3- and +1.5-fold; p < 0.05). Findings support, for the first time, the potential contribution of QMs, especially ISOR, in the regulation of lipid metabolism in vitro, possibly via AMPK activation. Further studies, including in vivo, are encouraged to strengthen evidence of the mechanisms observed.
{"title":"Effects of Quercetin Metabolites on Glucose-Dependent Lipid Accumulation in 3T3-L1 Adipocytes","authors":"Marco Rendine, Samuele Venturi, Mirko Marino, Claudio Gardana, Peter Møller, Daniela Martini, Patrizia Riso, Cristian Del Bo","doi":"10.1002/mnfr.70070","DOIUrl":"https://doi.org/10.1002/mnfr.70070","url":null,"abstract":"The aim of the study was to assess the effects of quercetin metabolites (QMs) on lipid accumulation in adipocytes under high-glucose and physiological-glucose concentrations and to elucidate the mechanisms involved. 3T3-L1 mature adipocytes were exposed to a physiological glucose concentration, as a model of caloric restriction (CR), or high glucose (control), with and without QMs (quercetin-3-glucuronide [Q3G] and isorhamnetin [ISOR]). Cells were treated with Q3G (0.3 and 0.6 µmol/L) and ISOR (0.2 and 0.4 µmol/L) for 48 h. Lipid accumulation (Oil Red O staining) and Δ glucose level (HPLC) were assessed. Under high glucose, Q3G and ISOR reduced lipid accumulation (−10.8% and −10.4%; <i>p</i> < 0.01) and Δ glucose level (−13.6% and −14.2%; <i>p</i> < 0.05). Under CR, QMs increased Δ glucose level (+21.6% for Q3G and +21% for ISOR; <i>p</i> < 0.05). ISOR increased pAMPK levels under high glucose (+1.4-fold; <i>p</i> < 0.05). Under CR, Q3G and ISOR increased pAMPK (+1.4- and +1.5-fold; <i>p</i> < 0.05), while ISOR upregulated SIRT1 and PGC-1α (+2.3- and +1.5-fold; <i>p</i> < 0.05). Findings support, for the first time, the potential contribution of QMs, especially ISOR, in the regulation of lipid metabolism in vitro, possibly via AMPK activation. Further studies, including in vivo, are encouraged to strengthen evidence of the mechanisms observed.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"28 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yushi Du, Shuangshuang Li, Guanyu Chen, Yihui Mao, Shasha Zhu, Wenyu Zhang, Mengxi Kang, Yi Sui, Dongliang Wang
Boosting apoptotic cell clearance by phagocytes including colonic epithelial cells (CECs), a process named efferocytosis, inhibits colitis development. Lactucopicrin (LCP), one common bitter sesquiterpene lactone affluent in leafy vegetables possesses a significant antiinflammatory property. However, it remains unknown whether LCP could regulate CECs efferocytosis and colitis development in vivo. Methods and Results: LCP (0.25-1 µmol/L) does not appreciably change the efferocytic capacity of murine primary CECs to clear apoptotic CECs. Instead, LCP dose-dependently increases the efferocytic capacity of CECs treated with butyrate (But). This effect is reliant on efferocytic receptor brain-specific angiogenesis 1 (BAI1). Although LCP does not significantly affect BAI1 expression, it alters BAI1 distribution with an increase in lipid raft microdomains in plasma membrane, an effect responsible for the LCP effect on efferocytic capacity. Moreover, dietary supplementation with 0.012% wt/wt of LCP attenuates dextran sulfate sodium (DSS)-induced colitis in C57BL/6J mice, along with an increase in efferocytic capacity of CECs and fecal But content, a reduction in apoptotic cell accumulation and inflammation burden in colonic tissues. Conclusion: Dietary LCP could inhibit DSS-induced colitis in mice, likely through enhancing BAI1-mediated efferocytosis of CECs, thus providing a new candidate for the treatment of colitis.
{"title":"Sesquiterpene Lactone Lactucopicrin Boosts Apoptotic Cell Clearance by Colonic Epithelial Cells and Alleviates Colitis in Mice","authors":"Yushi Du, Shuangshuang Li, Guanyu Chen, Yihui Mao, Shasha Zhu, Wenyu Zhang, Mengxi Kang, Yi Sui, Dongliang Wang","doi":"10.1002/mnfr.70062","DOIUrl":"https://doi.org/10.1002/mnfr.70062","url":null,"abstract":"Boosting apoptotic cell clearance by phagocytes including colonic epithelial cells (CECs), a process named efferocytosis, inhibits colitis development. Lactucopicrin (LCP), one common bitter sesquiterpene lactone affluent in leafy vegetables possesses a significant antiinflammatory property. However, it remains unknown whether LCP could regulate CECs efferocytosis and colitis development in vivo. Methods and Results: LCP (0.25-1 µmol/L) does not appreciably change the efferocytic capacity of murine primary CECs to clear apoptotic CECs. Instead, LCP dose-dependently increases the efferocytic capacity of CECs treated with butyrate (But). This effect is reliant on efferocytic receptor brain-specific angiogenesis 1 (BAI1). Although LCP does not significantly affect BAI1 expression, it alters BAI1 distribution with an increase in lipid raft microdomains in plasma membrane, an effect responsible for the LCP effect on efferocytic capacity. Moreover, dietary supplementation with 0.012% wt/wt of LCP attenuates dextran sulfate sodium (DSS)-induced colitis in C57BL/6J mice, along with an increase in efferocytic capacity of CECs and fecal But content, a reduction in apoptotic cell accumulation and inflammation burden in colonic tissues. Conclusion: Dietary LCP could inhibit DSS-induced colitis in mice, likely through enhancing BAI1-mediated efferocytosis of CECs, thus providing a new candidate for the treatment of colitis.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"16 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wei Siang, Jiang Li Jin, Jiao Yinming, Lin Wenji, Feng Yan
Alcoholic heart disease (AHD) is a severe cardiovascular condition linked to chronic alcohol consumption. This study investigates the effects of a high-fiber diet and acetate on gut microbiota and cardiac function in AHD mouse models. Sixty male C57BL/6 mice were divided into six groups, receiving either a control diet, high-fiber diet, or acetate supplementation alongside alcohol treatment. Results revealed that cardiac fibrosis and heart failure were notably improved in the AHD mice receiving high-fiber or acetate diets. Transcriptomic analyses indicated that dietary interventions modulated the expression of genes involved in lipid metabolism and the TGF-β signaling pathway. Additionally, 16S rRNA sequencing showed that the high-fiber diet and acetate altered gut microbiota composition, enhancing the abundance of beneficial bacteria such as Akkermansia muciniphila, Lactobacillus intestinalis, and Bacteroides acidifaciens. These microbes exhibited positive correlations with genes related to fat metabolism and TGF-β signaling, suggesting a potential mechanism for gut microbiota's role in AHD pathology. ROC analysis identified these bacteria as promising biomarkers for AHD detection. Overall, our findings underscore the therapeutic potential of dietary fiber and acetate in modulating gut microbiota and improving cardiac function in AHD, highlighting the intricate relationship between gut health and cardiovascular disease management.
{"title":"Effects of Dietary Fiber and Acetate on Alcoholic Heart Disease and Intestinal Microbes in Mice","authors":"Wei Siang, Jiang Li Jin, Jiao Yinming, Lin Wenji, Feng Yan","doi":"10.1002/mnfr.70040","DOIUrl":"https://doi.org/10.1002/mnfr.70040","url":null,"abstract":"Alcoholic heart disease (AHD) is a severe cardiovascular condition linked to chronic alcohol consumption. This study investigates the effects of a high-fiber diet and acetate on gut microbiota and cardiac function in AHD mouse models. Sixty male C57BL/6 mice were divided into six groups, receiving either a control diet, high-fiber diet, or acetate supplementation alongside alcohol treatment. Results revealed that cardiac fibrosis and heart failure were notably improved in the AHD mice receiving high-fiber or acetate diets. Transcriptomic analyses indicated that dietary interventions modulated the expression of genes involved in lipid metabolism and the TGF-β signaling pathway. Additionally, 16S rRNA sequencing showed that the high-fiber diet and acetate altered gut microbiota composition, enhancing the abundance of beneficial bacteria such as <i>Akkermansia muciniphila</i>, <i>Lactobacillus intestinalis</i>, and <i>Bacteroides acidifaciens</i>. These microbes exhibited positive correlations with genes related to fat metabolism and TGF-β signaling, suggesting a potential mechanism for gut microbiota's role in AHD pathology. ROC analysis identified these bacteria as promising biomarkers for AHD detection. Overall, our findings underscore the therapeutic potential of dietary fiber and acetate in modulating gut microbiota and improving cardiac function in AHD, highlighting the intricate relationship between gut health and cardiovascular disease management.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"37 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jinchen Li, Mengdi Lu, Hongying Huang, Tong Zhao, Huiyan Yu, Cui Zhou, Weiwei Ma
This study aimed to explore the role of fatty acids in regulating cognitive function through the gut microbiota (GM) and circulating microRNAs (miRNAs) by comprehensive analysis of dietary fatty acid intake (erythrocyte membrane fatty acid composition) and the GM and miRNAs in people with different body mass index (BMI). Participants with different BMIs in Beijing (normal weight [NW], overweight [OW], and obese [OB]) were recruited according to the Chinese obesity diagnostic criteria. Compared with the NW group, cognitive decline was observed in the OW and OB groups. Dietary saturated fatty acid (SFA) intake increased and dietary unsaturated fatty acid intake decreased in NW people. In the OW group, C18:3n-3 had beneficial mediation effects for cognition. Conversely, C18:3n-6 showed adverse cognitive effects in the mediating analysis. In the OW group, hsa-miR-142-5p presented a negative correlation with cognitive function. Erythrocyte membrane C23:0, as a good indicator of dietary fat intake, was found to influence cognitive function through Fusobacteriota, Proteobacteria, and plasma hsa-miR-144-3p in OB individuals. Different types of dietary fatty acid intake may affect the interaction between GM and miRNAs, and exert cognitive effects on middle-aged and elderly population, forming an interactive network of fatty acids-GM/miRNAs-cognition.
{"title":"Effect of the Fatty Acid Intake on Cognitive Function Through Gut Microbiota and Circulating microRNAs Remodeling in Middle-Aged and Elderly Overweight and Obese Population","authors":"Jinchen Li, Mengdi Lu, Hongying Huang, Tong Zhao, Huiyan Yu, Cui Zhou, Weiwei Ma","doi":"10.1002/mnfr.70027","DOIUrl":"https://doi.org/10.1002/mnfr.70027","url":null,"abstract":"This study aimed to explore the role of fatty acids in regulating cognitive function through the gut microbiota (GM) and circulating microRNAs (miRNAs) by comprehensive analysis of dietary fatty acid intake (erythrocyte membrane fatty acid composition) and the GM and miRNAs in people with different body mass index (BMI). Participants with different BMIs in Beijing (normal weight [NW], overweight [OW], and obese [OB]) were recruited according to the Chinese obesity diagnostic criteria. Compared with the NW group, cognitive decline was observed in the OW and OB groups. Dietary saturated fatty acid (SFA) intake increased and dietary unsaturated fatty acid intake decreased in NW people. In the OW group, C18:3<i>n</i>-3 had beneficial mediation effects for cognition. Conversely, C18:3<i>n</i>-6 showed adverse cognitive effects in the mediating analysis. In the OW group, hsa-miR-142-5p presented a negative correlation with cognitive function. Erythrocyte membrane C23:0, as a good indicator of dietary fat intake, was found to influence cognitive function through <i>Fusobacteriota</i>, <i>Proteobacteria</i>, and plasma hsa-miR-144-3p in OB individuals. Different types of dietary fatty acid intake may affect the interaction between GM and miRNAs, and exert cognitive effects on middle-aged and elderly population, forming an interactive network of fatty acids-GM/miRNAs-cognition.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"43 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lin-Feng Tang, Feng-Ling Tang, Hao Zhou, Ze-Kun Li, Chao-Qun Pi, Yang He, Ming Li
The gastrointestinal tract is highly sensitive to ionizing radiation (IR), which causes radiation-induced intestinal injury (RIII). There are no effective drugs available for RIII in routine clinical treatment, which is a major limiting factor during the process of radiotherapy for pelvic abdominal malignancies. In this study, we aimed to elucidate the potential of probiotic Bacillus coagulans BC99 (B.coagulans BC99) in preventing RIII. C57BL/6J mice were gavage-administered with B.coagulans BC99 for 30 days and then exposed to a single dose of 12 Gy x-ray whole abdominal irradiation (WAI). B.coagulans BC99 treatment could mitigate RIII by preventing weight loss, maintaining the integrity of intestinal structure and barrier, improving inflammatory symptoms, modulating oxidative stress, and regulating the composition of gut microbiota, thereby reestablishing intestinal homeostasis. In addition, the potential radioprotective mechanism of B.coagulans BC99 was closely related to the gut microbiota-derived metabolites. This study offers a novel perspective for advancing probiotic-based treatments for RIII and enhancing strategies for the prevention of RIII.
{"title":"Bacillus Coagulans BC99 Protects Ionizing Radiation-Induced Intestinal Injury and Modulates Gut Microbiota and Metabolites in Mice","authors":"Lin-Feng Tang, Feng-Ling Tang, Hao Zhou, Ze-Kun Li, Chao-Qun Pi, Yang He, Ming Li","doi":"10.1002/mnfr.70057","DOIUrl":"https://doi.org/10.1002/mnfr.70057","url":null,"abstract":"The gastrointestinal tract is highly sensitive to ionizing radiation (IR), which causes radiation-induced intestinal injury (RIII). There are no effective drugs available for RIII in routine clinical treatment, which is a major limiting factor during the process of radiotherapy for pelvic abdominal malignancies. In this study, we aimed to elucidate the potential of probiotic <i>Bacillus coagulans</i> BC99 (<i>B.coagulans</i> BC99) in preventing RIII. C57BL/6J mice were gavage-administered with <i>B.coagulans</i> BC99 for 30 days and then exposed to a single dose of 12 Gy x-ray whole abdominal irradiation (WAI). <i>B.coagulans</i> BC99 treatment could mitigate RIII by preventing weight loss, maintaining the integrity of intestinal structure and barrier, improving inflammatory symptoms, modulating oxidative stress, and regulating the composition of gut microbiota, thereby reestablishing intestinal homeostasis. In addition, the potential radioprotective mechanism of <i>B.coagulans</i> BC99 was closely related to the gut microbiota-derived metabolites. This study offers a novel perspective for advancing probiotic-based treatments for RIII and enhancing strategies for the prevention of RIII.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"108 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Naringenin, a flavonoid widely present in citrus fruits, has garnered considerable attention due to its diverse biological activities and health-promoting benefits. As research on naringenin advances, the application scope of naringenin has significantly expanded. This paper provides a systematic overview of the production and synthesis methods of naringenin, focusing especially on the application of green extraction techniques and the strategies for constructing microbial metabolic engineering. Naringenin not only achieves its diverse biological activities including antioxidant, antiinflammatory, and glucolipid metabolism regulation through multiple mechanisms but also modulates the balance of gut microbiota, thereby mediating synergistic health effects via the host–microbial metabolic axis. Given the low oral bioavailability of naringenin, various nanodelivery systems have been developed to improve its bioavailability. Meanwhile, molecular simulation techniques elucidate the binding conformation characteristics with receptors at the molecular level, providing novel insights into its mechanisms of action. In conclusion, this review seeks to offer a theoretical basis and future directions for further research and application of naringenin.
{"title":"Novel Insights Into Naringenin: A Multifaceted Exploration of Production, Synthesis, Health Effects, Nanodelivery Systems, and Molecular Simulation","authors":"Yuanqiang Jia, Xinjing Zhou, Yanan Liu, Xiaoyong Liu, Feiyue Ren, Hongzhi Liu","doi":"10.1002/mnfr.70066","DOIUrl":"https://doi.org/10.1002/mnfr.70066","url":null,"abstract":"Naringenin, a flavonoid widely present in citrus fruits, has garnered considerable attention due to its diverse biological activities and health-promoting benefits. As research on naringenin advances, the application scope of naringenin has significantly expanded. This paper provides a systematic overview of the production and synthesis methods of naringenin, focusing especially on the application of green extraction techniques and the strategies for constructing microbial metabolic engineering. Naringenin not only achieves its diverse biological activities including antioxidant, antiinflammatory, and glucolipid metabolism regulation through multiple mechanisms but also modulates the balance of gut microbiota, thereby mediating synergistic health effects via the host–microbial metabolic axis. Given the low oral bioavailability of naringenin, various nanodelivery systems have been developed to improve its bioavailability. Meanwhile, molecular simulation techniques elucidate the binding conformation characteristics with receptors at the molecular level, providing novel insights into its mechanisms of action. In conclusion, this review seeks to offer a theoretical basis and future directions for further research and application of naringenin.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"6 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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