[The effect of exercise on platelet-activating factor metabolism in the livers of rats fed high-fat diet].

Q3 Medicine 生理学报 Pub Date : 2024-08-25
Shang-Yan Dong, Wei Li, Li-Feng Wang, Zhen-Mao Lin, Ming-Jian Chen, Ting Li
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Abstract

This paper aimed to investigate the effects of exercise on hepatic platelet-activating factor (PAF) metabolism in rats fed a high-fat diet. Thirty-two male Sprague-Dawley (SD) rats were divided into control group (C), high-fat diet group (H), exercise group (EC), and high-fat diet+exercise group (EH). Serum lipids, glucose, insulin and markers of hepatic injury after a 16-week dietary and/or exercise intervention (60 min/day, 6 times/week) were measured by biochemical analysis; liver lipidomic profiles were analyzed by liquid chromatograph-mass spectrometer (LC-MS). Gene and protein expression of enzymes related to PAF metabolism were determined by qPCR and Western blot respectively. The results showed that high-fat diet feeding significantly increased the levels of low-density lipoprotein-cholesterol (LDL-C) and liver injury markers including purine nucleoside phosphorylase (PNP) and malondialdehyde (MDA) in rats, which were decreased by exercise. Furthermore, high-fat diet feeding significantly increased the hepatic PAF content, which was also attenuated by exercise. In addition, although high-fat diet treatment resulted in an increase in the expression of both PAF synthetase (PAF-CPT and PLA2) and hydrolase (Lp-PLA2 and PAF-AH(II)), induction of PAF synthetase was much greater than that of PAF hydrolase. While exercise increased the expression of Lp-PLA2 and PAF-AH(II) and decreased the expression of PAF-CPT and PLA2, key PAF synthesizing enzymes. In conclusion, high-fat diet-induced increase in hepatic PAF content is mainly due to the increase of its pathological synthesis at the translational level. Exercise reduces hepatic PAF content in high-fat fed rats by increasing PAF hydrolysis and decreasing its synthesis.

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[运动对高脂饮食大鼠肝脏中血小板活化因子代谢的影响]。
本文旨在研究运动对高脂饮食大鼠肝脏血小板活化因子(PAF)代谢的影响。32只雄性Sprague-Dawley(SD)大鼠被分为对照组(C)、高脂饮食组(H)、运动组(EC)和高脂饮食+运动组(EH)。通过生化分析测定大鼠在接受为期 16 周的饮食和/或运动干预(60 分钟/天,6 次/周)后的血清脂质、葡萄糖、胰岛素和肝损伤指标;通过液相色谱-质谱仪(LC-MS)分析肝脏脂质组谱。通过 qPCR 和 Western 印迹分别测定了 PAF 代谢相关酶的基因和蛋白表达。结果表明,高脂饮食能显著增加大鼠低密度脂蛋白胆固醇(LDL-C)和肝损伤标志物(包括嘌呤核苷磷酸化酶(PNP)和丙二醛(MDA))的水平,而运动能降低这些标志物的水平。此外,高脂饮食会显著增加肝脏 PAF 含量,而运动也会减少 PAF 含量。此外,虽然高脂饮食会导致 PAF 合成酶(PAF-CPT 和 PLA2)和水解酶(Lp-PLA2 和 PAF-AH(II))的表达增加,但 PAF 合成酶的诱导作用远大于 PAF 水解酶的诱导作用。运动增加了 Lp-PLA2 和 PAF-AH(II)的表达,降低了 PAF-CPT 和 PLA2(PAF 合成的关键酶)的表达。总之,高脂饮食诱导的肝脏 PAF 含量增加主要是由于其在转化水平上的病理性合成增加。运动可通过增加 PAF 的水解和减少其合成来降低高脂喂养大鼠肝脏 PAF 的含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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