Aptamer regulated peroxidase-like activity of cobalt oxyhydroxide nanosheets for colorimetric detection of kanamycin.

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL Analytical Sciences Pub Date : 2024-08-28 DOI:10.1007/s44211-024-00655-y
Shuyan Xiao, Jiafeng Wu, Mingqin Kang, Zhongping Dong
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Abstract

A straightforward label-free colorimetric aptasensor utilizing the aptamer-enhanced peroxidase-like activity of cobalt oxyhydroxide (CoOOH) nanosheets has been established for kanamycin detection. In the kanamycin-free state, aptamers adsorb onto the CoOOH surface through electrostatic forces, enhancing the peroxidase-like activity of CoOOH and thereby resulting in a strong absorption signal and a yellow hue in 3,3',5,5'-tetramethylbenzidine (TMB) upon termination of the reaction with a stop solution. Conversely, upon the introduction of kanamycin, aptamers and CoOOH nanosheets compete for binding to kanamycin, resulting in a significant decrease in the number of aptamers bound to CoOOH. As a result, the activity of CoOOH diminishes, leading to a corresponding reduction in coloration and absorbance of the solution. Hence, the quantitative determination of kanamycin could be realized by analyzing the absorbance variations. Under optimal conditions, the aptasensor demonstrated high sensitivity and specificity, with a linear detection range from 500 nM to 5 µM and a detection limit as low as 54.6 nM. Moreover, the aptasensor effectively identified kanamycin in river water samples, achieving a recovery rate between 91.7% and 102.1%. This approach offers good practicability and provides a novel platform for kanamycin detection in environmental samples.

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用于卡那霉素比色检测的氢氧化钴纳米薄片的过氧化物酶样活性受色素调节。
利用纳米氧化钴(CoOOOH)的合体增强过氧化物酶样活性,建立了一种直接的无标记比色合体传感器,用于检测卡那霉素。在不含卡那霉素的状态下,适配体通过静电力吸附在 CoOOH 表面,增强了 CoOOH 的过氧化物酶样活性,因此在用停止液终止反应后,3,3',5,5'-四甲基联苯胺(TMB)会产生强烈的吸收信号和黄色色调。相反,引入卡那霉素后,适配体和 CoOOH 纳米片会竞争性地与卡那霉素结合,导致与 CoOOH 结合的适配体数量显著减少。因此,CoOOH 的活性降低,导致溶液的着色和吸光度也相应降低。因此,可以通过分析吸光度的变化来实现卡那霉素的定量测定。在最佳条件下,该灵敏度和特异性都很高,线性检测范围为 500 nM 至 5 µM,检测限低至 54.6 nM。此外,该灵敏传感器还能有效鉴定河水样本中的卡那霉素,回收率在 91.7% 到 102.1% 之间。该方法具有良好的实用性,为环境样品中卡那霉素的检测提供了一个新平台。
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来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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