Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes.

IF 5.1 2区生物学 Q2 CELL BIOLOGY Cells Pub Date : 2024-08-17 DOI:10.3390/cells13161373

Hiroshi Yamada, Hirona Osaka, Nanami Tatsumi, Miu Araki, Tadashi Abe, Keiko Kaihara, Ken Takahashi, Eizo Takashima, Takayuki Uchihashi, Keiji Naruse, Kohji Takei

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Abstract

Synaptopodin 2-like protein (SYNPO2L) is localized in the sarcomere of cardiomyocytes and is involved in heart morphogenesis. However, the molecular function of SYNPO2L in the heart is not fully understood. We investigated the interaction of SYNPO2L with sarcomeric α-actinin and actin filaments in cultured mouse cardiomyocytes. Immunofluorescence studies showed that SYNPO2L colocalized with α-actinin and actin filaments at the Z-discs of the sarcomere. Recombinant SYNPO2La or SYNPO2Lb caused a bundling of the actin filaments in the absence of α-actinin and enhanced the α-actinin-dependent formation of actin bundles. In addition, high-speed atomic force microscopy revealed that SYNPO2La directly bound to α-actinin via its globular ends. The interaction between α-actinin and SYNPO2La fixed the movements of the two proteins on the actin filaments. These results strongly suggest that SYNPO2L cooperates with α-actinin during actin bundle formation to facilitate sarcomere formation and maintenance.

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突触素 2 类似蛋白与 Alpha-Actinin 的直接结合有助于心肌细胞中肌动蛋白束的形成

突触素 2 样蛋白（SYNPO2L）定位于心肌细胞的肌节中，参与心脏的形态发生。然而，SYNPO2L 在心脏中的分子功能尚不完全清楚。我们在培养的小鼠心肌细胞中研究了SYNPO2L与肉瘤α-肌动蛋白和肌动蛋白丝的相互作用。免疫荧光研究表明，SYNPO2L 与α-肌动蛋白和肌动蛋白丝共定位在肌节的 Z 盘处。重组 SYNPO2La 或 SYNPO2Lb 能在α-肌动蛋白缺失的情况下导致肌动蛋白丝成束，并能增强α-肌动蛋白依赖的肌动蛋白束的形成。此外，高速原子力显微镜显示，SYNPO2La 通过其球状末端直接与 α-肌动蛋白结合。α-肌动蛋白和 SYNPO2La 之间的相互作用固定了这两种蛋白质在肌动蛋白丝上的运动。这些结果有力地表明，SYNPO2L 在肌动蛋白束形成过程中与α-肌动蛋白合作，促进了肌节的形成和维持。

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来源期刊

Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

9.90

自引率

5.00%

发文量

3472

审稿时长

16 days

期刊介绍： Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.