Osteogenic Protection against Fine Dust with Erucic Acid-Induced Exosomes.

IF 5 3区医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2024-07-31 DOI:10.3390/jfb15080215

Hyunjung Kim, Boyong Kim

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Abstract

Fine dust causes various disorders, including cardiovascular, neurological, renal, reproductive, motor, systemic, respiratory, and cancerous diseases. Therefore, it is essential to study functional materials to prevent these issues. This study investigated the beneficial effects of erucic acid against fine dust using methods such as miRNA profiling, quantitative PCR, flow cytometry, ELISA, and Alizarin O staining. Erucic acid effectively suppresses inflammation and upregulates osteogenic activators in fibroblasts exposed to fine dust. Additionally, erucic acid-induced exosomes (EIEs) strongly counteract the negative effects of fine dust on osteocytic differentiation and inflammation. Despite fine dust exposure, EIEs promoted osteocytic differentiation in adipose-derived stem cells (ASCs) and enhanced osteogenesis and phagocytosis in macrophages. The significant upregulation of RunX2 and BMP7 by EIEs indicates its strong role in osteocytic differentiation and protection against the effects of fine dust. EIEs also boosts immune activity and acts as an osteogenic trigger for macrophages. MicroRNA profiling revealed that EIEs dramatically upregulated miRNAs, including hsa-miRNA-1301-3p, hsa-miRNA-1908-5p, hsa-miRNA-423-5p, and hsa-miRNA-122-5p, which are associated with osteogenic differentiation and immunity. Therefore, EIEs show potential as biomaterials to prevent environment-borne diseases.

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用芥子酸诱导的外泌体保护骨骼免受微尘侵袭

微尘会导致各种疾病，包括心血管、神经、肾脏、生殖、运动、系统、呼吸和癌症疾病。因此，研究预防这些问题的功能性材料至关重要。本研究采用 miRNA 图谱分析、定量 PCR、流式细胞仪、ELISA 和茜素 O 染色等方法，研究了芥酸对微尘的有益作用。芥酸能有效抑制炎症，并上调暴露于微尘的成纤维细胞中的成骨激活因子。此外，芥酸诱导的外泌体（EIEs）能有效抵消微尘对成骨细胞分化和炎症的负面影响。尽管暴露于微尘，EIEs仍能促进脂肪来源干细胞（ASCs）的成骨细胞分化，并增强巨噬细胞的成骨和吞噬功能。EIEs对RunX2和BMP7的明显上调表明，EIEs在成骨细胞分化和抵御微尘影响方面发挥了强有力的作用。EIEs还能增强免疫活性，并作为巨噬细胞的成骨触发器。微RNA分析表明，EIEs显著上调了与成骨分化和免疫相关的miRNA，包括hsa-miRNA-1301-3p、hsa-miRNA-1908-5p、hsa-miRNA-423-5p和hsa-miRNA-122-5p。因此，EIEs 具有作为生物材料预防环境传播疾病的潜力。

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.