Comparative Analysis of the Mitochondrial Genome Sequences of Diaporthe longicolla (syn. Phomopsis longicolla) Isolates Causing Phomopsis Seed Decay in Soybean.

IF 4.2 2区生物学 Q2 MICROBIOLOGY Journal of Fungi Pub Date : 2024-08-13 DOI:10.3390/jof10080570

Shuxian Li, Xiaojun Hu, Qijian Song

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Abstract

Diaporthe longicolla (syn. Phomopsis longicolla) is an important seed-borne fungal pathogen and the primary cause of Phomopsis seed decay (PSD) in soybean. PSD is one of the most devastating seed diseases, reducing soybean seed quality and yield worldwide. As part of a genome sequencing project on the fungal Diaporthe-Phomopsis complex, draft genomes of eight D. longicolla isolates were sequenced and assembled. Sequences of mitochondrial genomes were extracted and analyzed. The circular mitochondrial genomes ranged from 52,534 bp to 58,280 bp long, with a mean GC content of 34%. A total of 14 core protein-coding genes, 23 tRNA, and 2 rRNA genes were identified. Introns were detected in the genes of atp6, cob, cox1, cox2, cox3, nad1, nad2, nad5, and rnl. Three isolates (PL7, PL10, and PL185E) had more introns than other isolates. Approximately 6.4% of the mitochondrial genomes consist of repetitive elements. Moreover, 48 single-nucleotide polymorphisms (SNPs) and were identified. The mitochondrial genome sequences of D. longicolla will be useful to further study the molecular basis of seed-borne pathogens causing seed diseases, investigate genetic variation among isolates, and develop improved control strategies for Phomopsis seed decay of soybean.

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导致大豆种子腐烂的 Diaporthe longicolla（同属 Phomopsis longicolla）分离株线粒体基因组序列比较分析。

Diaporthe longicolla（同属 Phomopsis longicolla）是一种重要的种子传播真菌病原体，也是导致大豆种子腐烂（PSD）的主要原因。PSD 是最具破坏性的种子病害之一，会降低全球大豆种子的质量和产量。作为真菌 Diaporthe-Phomopsis 复合体基因组测序项目的一部分，对八个 D. longicolla 分离物的基因组草案进行了测序和组装。提取并分析了线粒体基因组序列。环形线粒体基因组长度从 52,534 bp 到 58,280 bp 不等，平均 GC 含量为 34%。共鉴定出 14 个核心蛋白质编码基因、23 个 tRNA 和 2 个 rRNA 基因。在 atp6、cob、cox1、cox2、cox3、nad1、nad2、nad5 和 rnl 的基因中检测到了中子。三个分离株（PL7、PL10 和 PL185E）的内含子数量多于其他分离株。约 6.4% 的线粒体基因组由重复元件组成。此外，还发现了 48 个单核苷酸多态性（SNPs）。D. longicolla 的线粒体基因组序列将有助于进一步研究引起种子病害的种子传播病原体的分子基础，调查分离株之间的遗传变异，并开发出更好的大豆拟褐飞虱种子腐烂病防治策略。

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来源期刊

Journal of Fungi Medicine-Microbiology (medical)

CiteScore

6.70

自引率

14.90%

发文量

1151

审稿时长

11 weeks

期刊介绍： Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.