Imaging Diffractometric Biosensors for Label-Free, Multi-Molecular Interaction Analysis.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-08-17 DOI:10.3390/bios14080398
Cornelia Reuter, Walter Hauswald, Sindy Burgold-Voigt, Uwe Hübner, Ralf Ehricht, Karina Weber, Juergen Popp
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Abstract

Biosensors are used for the specific and sensitive detection of biomolecules. In conventional approaches, the suspected target molecules are bound to selected capture molecules and successful binding is indicated by additional labelling to enable optical readout. This labelling requires additional processing steps tailored to the application. While numerous label-free interaction assays exist, they often compromise on detection characteristics. In this context, we introduce a novel diffractometric biosensor, comprising a diffractive biosensor chip and an associated optical reader assembly. This innovative system can capture an entire assay, detecting various types of molecules in a label-free manner and present the results within in a single, comprehensive image. The applicability of the biosensor is assessed for the detection of viral DNA as well as proteins directly in human plasma, investigating different antigens. In our experiments, we achieve a detection limit of 4.2 pg/mm², which is comparable to other label-free optical biosensors. The simplicity and robustness of the method make it a compelling option for advancing biosensing technologies. This work contributes to the development of an imaging diffractometric biosensor with the potential for multiple applications in molecular interaction analysis.

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用于无标记、多分子相互作用分析的成像衍射生物传感器。
生物传感器用于对生物分子进行特异而灵敏的检测。在传统方法中,可疑的目标分子与选定的捕获分子结合,并通过额外的标记来表明结合成功,从而实现光学读数。这种标记需要根据应用情况进行额外的处理步骤。虽然存在许多无标记相互作用检测方法,但它们往往在检测特性上大打折扣。在这种情况下,我们推出了一种新型衍射生物传感器,由衍射生物传感器芯片和相关的光学读取器组件组成。这种创新系统可以捕捉整个检测过程,以无标记方式检测各种类型的分子,并将检测结果以单一、全面的图像呈现出来。我们评估了这种生物传感器的适用性,它可以直接检测人体血浆中的病毒 DNA 和蛋白质,并对不同的抗原进行研究。在实验中,我们的检测限达到了 4.2 pg/mm²,与其他无标记光学生物传感器相当。该方法的简便性和稳健性使其成为推动生物传感技术发展的一个令人信服的选择。这项工作有助于开发一种成像衍射生物传感器,它在分子相互作用分析中具有多种应用潜力。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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