A new insight for investigating the prenatal and postnatal ossification centers of pelvic and femur bones in white New Zealand rabbits (Oryctolagus cuniculus) using 3D CT, double stain technique, and morphometry

IF 2 3区 医学 Q2 ANATOMY & MORPHOLOGY Annals of Anatomy-Anatomischer Anzeiger Pub Date : 2024-08-25 DOI:10.1016/j.aanat.2024.152316
Basma M. Kamal , Samir A.A. El-Gendy , Ahmed M. Rashwan , Hanan H. Abd-Elhafeez , Soha Soliman , Neveen E.R. El-Bakary , Ahmed A. El-Mansi , Mamdouh B. Eldesoqui , Mohamed A.M. Alsafy
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Abstract

Background

The ossification centers in rabbit limbs are related to fetal age and bone maturation. Objective: To address the limited studies on ossification in the hind limbs of New Zealand rabbits, we investigated the prenatal and postnatal development of the pelvic and femur bones.

Methods

Double staining with Alcian Blue and Alizarin Red, computed tomography (CT), and 3D reconstruction were employed to visualize and analyze ossification centers in detail.

Results

Using double staining, we observed these patterns: At prenatal days 18 and 21, ossification centers appeared in the ilium. By prenatal days 23 and 25, ossification began in the ischium. On postnatal day 1, ilium ossification centers spread across most of the ilium wings, except for the iliac crest, and new centers appeared in the pubis and cotyloid bones. Most bones had ossified by the third week and one month postnatal, except for the iliac crest and ischial tuberosity. At 1.5 months, both were fully ossified. On day 18 post coitum, an ossification center was visible in the middle of the femur shaft. By day 28 post coitum, ossification extended through the shaft, and postnatally, new ossification spots appeared at the extremities by day one and week one. By the third week, complete ossification of the femur head, lesser trochanter, third trochanter, medial condyle, and lateral condyle was observed. At 1.5 months, the entire proximal extremity was ossified.

Conclusion

3D CT provided clear imaging of ossification progression in the pelvic and femur bones. This study enhances our understanding of vertebrate skeletal development.

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利用三维 CT、双染色技术和形态计量学研究新西兰白兔骨盆和股骨产前和产后骨化中心的新见解。
背景:兔四肢的骨化中心与胎龄和骨成熟度有关:兔子四肢的骨化中心与胎龄和骨骼成熟度有关:针对新西兰兔后肢骨化研究有限的问题,我们研究了骨盆骨和股骨的产前和产后发育情况:方法:采用阿尔新蓝和茜素红双重染色法、计算机断层扫描(CT)和三维重建技术来观察和分析骨化中心的详细情况:通过双重染色,我们观察到了这些模式:在出生前第 18 和 21 天,髂骨出现骨化中心。在出生前第 23 天和第 25 天,骨化中心开始出现在髂骨。在出生后第 1 天,髂骨骨化中心遍布除髂嵴外的大部分髂翼,耻骨和楔骨也出现了新的骨化中心。到出生后第三周和一个月时,除髂嵴和峡部结节外,大多数骨骼都已骨化。在 1 个半月时,这两块骨骼完全骨化。产后第 18 天,股骨柄中部可见骨化中心。出生后第28天,骨化延伸至股骨柄,出生后第1天和第1周,四肢出现新的骨化点。第三周时,股骨头、小转子、第三转子、内侧髁和外侧髁完全骨化。结论:三维 CT 提供了骨盆和股骨骨化进展的清晰图像。这项研究加深了我们对脊椎动物骨骼发育的了解。
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来源期刊
Annals of Anatomy-Anatomischer Anzeiger
Annals of Anatomy-Anatomischer Anzeiger 医学-解剖学与形态学
CiteScore
4.40
自引率
22.70%
发文量
137
审稿时长
33 days
期刊介绍: Annals of Anatomy publish peer reviewed original articles as well as brief review articles. The journal is open to original papers covering a link between anatomy and areas such as •molecular biology, •cell biology •reproductive biology •immunobiology •developmental biology, neurobiology •embryology as well as •neuroanatomy •neuroimmunology •clinical anatomy •comparative anatomy •modern imaging techniques •evolution, and especially also •aging
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