CCKR signaling map, G-Protein bindings, hormonal regulation, and neural mechanisms may influence the osteogenic/cementogenic differentiation potential of hPDLSCs

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Archives of oral biology Pub Date : 2024-08-23 DOI:10.1016/j.archoralbio.2024.106069
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引用次数: 0

Abstract

Objective

Periodontal regeneration poses challenges due to the periodontium's complexity, relying on mesenchymal cells from the periodontal ligament (hPDLSCs) to regenerate hard tissues like bone and cementum. While some hPDLSCs have high regeneration potential (HOP-hPDLSCs), most are low potential (LOP-hPDLSCs). This study analyzed hPDLSCs from a single donor to minimize inter-individual variability and focus on key differences in differentiation potentials.

Design

This study used RNA-seq, genomic databases, and bioinformatics tools to explore signaling pathways (SPs), biological processes (BPs), and molecular functions (MFs) guiding HOP cells to mineralized matrix production. It also investigated limitations of LOP cells and strategies for enhancing their osteo/cementogenesis.

Results

In basal conditions, HOP exhibited a multifunctional gene network with higher expression of genes related to osteo/cementogenesis, cell differentiation, immune modulation, stress response, and hormonal regulation. In contrast, LOP focused on steroid hormone biosynthesis and nucleic acid maintenance. During osteo/cementogenic induction, HOP showed strong modulation of genes related to angiogenesis, cell division, mesenchymal differentiation, and extracellular matrix production. LOP demonstrated neural synaptic-related processes and preserved cellular cytoskeleton integrity. CCKR map signaling and G-protein receptor bindings gained significance during osteo/cementogenesis in HOP-hPDLSCs. Both HOP and LOP shared common BPs related to gastrointestinal and reproductive system development.

Conclusion

The osteo/cementogenic differentiation of HOP cells may be regulated by CCKR signaling, G-protein bindings, and specific hormonal regulation. LOP cells seem committed to neural mechanisms. This study sheds light on hPDLSCs' complex characteristics, offering a deeper understanding of their differentiation potential for future periodontal regeneration research and therapies.

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CCKR信号图谱、G蛋白结合、激素调控和神经机制可能会影响hPDLSCs的成骨/软骨分化潜能
目标由于牙周的复杂性,牙周再生面临着挑战,需要依靠牙周韧带的间充质细胞(hPDLSCs)来再生骨和骨水泥等硬组织。虽然有些 hPDLSCs 具有高再生潜能(HOP-hPDLSCs),但大多数 hPDLSCs 的再生潜能较低(LOP-hPDLSCs)。本研究分析了来自单一供体的hPDLSCs,以尽量减少个体间的差异,并关注分化潜能的关键差异。本研究使用RNA-seq、基因组数据库和生物信息学工具来探索引导HOP细胞产生矿化基质的信号通路(SP)、生物过程(BP)和分子功能(MF)。结果在基础条件下,HOP表现出多功能基因网络,与骨/骨水泥生成、细胞分化、免疫调节、应激反应和激素调节相关的基因表达较高。相比之下,LOP侧重于类固醇激素的生物合成和核酸维护。在骨/软骨诱导过程中,HOP 对与血管生成、细胞分裂、间充质分化和细胞外基质生成有关的基因有很强的调节作用。LOP 显示了与神经突触相关的过程,并保持了细胞细胞骨架的完整性。CCKR图信号转导和G蛋白受体结合在HOP-hPDLSCs的骨/软骨生成过程中具有重要意义。结论 HOP细胞的骨/软骨形成分化可能受CCKR信号、G蛋白结合和特定激素调控。LOP 细胞似乎致力于神经机制。本研究揭示了 hPDLSCs 的复杂特性,为未来牙周再生研究和治疗提供了对其分化潜力的更深入了解。
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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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