Interaction of high lipogenic states with titanium on osteogenesis

IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Bone Pub Date : 2024-08-28 DOI:10.1016/j.bone.2024.117242
T.S. Pinto , B.C. van der Eerden , M. Schreuders-Koedam , J. van de Peppel , I. Ayada , Q. Pan , M.M. Verstegen , L.J. van der Laan , G.M. Fuhler , W.F. Zambuzzi , M.P. Peppelenbosch
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Abstract

As obesity rates continue to rise, the prevalence of metabolic dysfunction and alcohol-associated steatotic liver disease (MetALD), a new term for Nonalcoholic Fatty Liver Disease (NAFLD), also increases. In an aging population, it is crucial to understand the interplay between metabolic disorders, such as MetALD, and bone health. This understanding becomes particularly significant in the context of implant osseointegration. This study introduces an in vitro model simulating high lipogenesis through the use of human Mesenchymal Stroma Cells-derived adipocytes, 3D intrahepatic cholangiocyte organoids (ICO), and Huh7 hepatocytes, to evaluate the endocrine influence on osteoblasts interacting with titanium. We observed a significant increase in intracellular fat accumulation in all three cell types, along with a corresponding elevation in metabolic gene expression compared to the control groups. Notably, osteoblasts undergoing mineralization in this high-lipogenesis environment also displayed lipid vesicle accumulation. The study further revealed that titanium surfaces modulate osteogenic gene expression and impact cell cycle progression, cell survival, and extracellular matrix remodeling under lipogenic conditions. These findings provide new insights into the challenges of implant integration in patients with obesity and MetALD, offering a deeper understanding of the metabolic influences on bone regeneration and implant success.

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高脂状态与钛对成骨的相互作用
随着肥胖率的持续上升,代谢功能障碍和酒精相关性脂肪肝(MetALD)(非酒精性脂肪肝的新名称)的发病率也在增加。在老龄化人口中,了解代谢紊乱(如 MetALD)与骨骼健康之间的相互作用至关重要。这种认识对种植体骨结合尤为重要。本研究通过使用人间质基质细胞衍生的脂肪细胞、三维肝内胆管细胞器质(ICO)和 Huh7 肝细胞,引入了一个模拟高脂生成的体外模型,以评估内分泌对与钛相互作用的成骨细胞的影响。与对照组相比,我们观察到所有三种细胞类型的细胞内脂肪积累都明显增加,代谢基因表达也相应升高。值得注意的是,在这种高脂质生成环境中进行矿化的成骨细胞也显示出脂质囊泡的积累。研究进一步揭示,在脂质生成条件下,钛表面会调节成骨基因表达,影响细胞周期进展、细胞存活和细胞外基质重塑。这些发现为肥胖症和 MetALD 患者种植体整合所面临的挑战提供了新的见解,使人们对骨质再生和种植体成功的代谢影响有了更深入的了解。
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来源期刊
Bone
Bone 医学-内分泌学与代谢
CiteScore
8.90
自引率
4.90%
发文量
264
审稿时长
30 days
期刊介绍: BONE is an interdisciplinary forum for the rapid publication of original articles and reviews on basic, translational, and clinical aspects of bone and mineral metabolism. The Journal also encourages submissions related to interactions of bone with other organ systems, including cartilage, endocrine, muscle, fat, neural, vascular, gastrointestinal, hematopoietic, and immune systems. Particular attention is placed on the application of experimental studies to clinical practice.
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