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Biomimetic periosteum-bone scaffolds with codelivery of BMP-2 and PDGF-BB for skull repair 生物仿真骨膜-骨支架与 BMP-2 和 PDGF-BB 共同输送用于颅骨修复。
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-11-04 DOI: 10.1016/j.bone.2024.117315
Tissue engineering employs the use of bioactive materials to facilitate the filling and acceleration of bone defect healing, thereby introducing novel concepts to the field of in situ bone repair. Some studies have shown that periosteum plays an important role in bone regeneration and repair. In this study, biomimetic periosteum-bone scaffolds were prepared by depositing poly-L-lactic acid (PLLA) electrospun fibers on the surface of the gelatin/chitosan cryogel to mimic the bone and periosteum structure, respectively. To improve the bioactivity of the scaffold, bone morphogenetic protein-2 (BMP-2) was loaded into a loose porous mesh-like cryogel, while platelet-derived growth factor-BB (PDGF-BB) was encapsulated in the core of PLLA nanofibers with core-shell structure. Both of these two growth factors were released locally at the site of bone defect, where they exert a synergistic effect on osteogenesis, thereby greatly accelerating bone healing. The in vitro experiments demonstrated that the biomimetic periosteum-bone scaffolds exhibited favourable biocompatibility and osteogenesis ability. Furthermore, the in vivo experiments indicated that the composite scaffold repaired rat skull defects in a more rapid and effective manner. In conclusion, biomimetic periosteum-bone scaffolds with codelivery of BMP-2 and PDGF-BB shows significant potential for bone regeneration.
组织工程学利用生物活性材料促进骨缺损的填充和加速愈合,从而为原位骨修复领域引入了新的概念。一些研究表明,骨膜在骨再生和修复中发挥着重要作用。本研究通过在明胶/壳聚糖低温凝胶表面沉积聚左旋乳酸(PLLA)电纺纤维,分别模拟骨和骨膜结构,制备了生物仿真骨膜-骨支架。为了提高支架的生物活性,骨形态发生蛋白-2(BMP-2)被载入疏松多孔的网状冷凝胶中,而血小板衍生生长因子-BB(PDGF-BB)则被包裹在具有核壳结构的 PLLA 纳米纤维的核心中。这两种生长因子在骨缺损部位局部释放,对骨生成产生协同作用,从而大大加速了骨愈合。体外实验表明,仿生骨膜-骨支架具有良好的生物相容性和成骨能力。此外,体内实验表明,复合支架能更快速、更有效地修复大鼠颅骨缺损。总之,共同递送 BMP-2 和 PDGF-BB 的仿生骨膜-骨支架在骨再生方面具有巨大潜力。
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引用次数: 0
Exercise-induced interactions between skeletal muscle and bone via myokines and osteokine in mice: Role of FNDC5/irisin, IGF-1, and osteocalcin 运动通过肌动蛋白和骨激肽诱导小鼠骨骼肌和骨骼之间的相互作用:FNDC5/鸢尾素、IGF-1 和骨钙素的作用
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-31 DOI: 10.1016/j.bone.2024.117314
Skeletal muscle and bone interact to maintain their structure and function. Physical exercise is the most effective and easily applicable strategy to maintain their functions; however, exercise-induced interactions by soluble factors remained elusive. Our study aimed to identify exercise-induced interactions between muscle and bone by examining (1) the effects of myokine on bone and (2) the effects of osteocalcin (OCN) on skeletal muscle. To understand the effects of exercise-induced myokines on bone, we examined the effects of FNDC5 for aerobic exercise and IGF-1 for resistance exercise using a muscle-specific myokine overexpression model. To examine OCN effects on muscle, mice were intraperitoneally administered OCN-neutralizing antibody during long-term exercise. Our result showed that aerobic exercise tended to increase serum HA-tag protein attached to FNDC5 in muscle-specific overexpression groups. In addition, osteoblastic activation was increased only after aerobic exercise with HA/FNDC5 overexpression. Resistance exercise did not alter circulating HA-tag (muscle-derived IGF-1) and bone metabolism after IGF-1/HA overexpression. In the OCN study, aerobic exercise enhanced endurance capacity by restoring muscle glycogen content; however, OCN neutralization returned these to baseline. After resistance exercise, OCN suppression inhibited muscle hypertrophy and strength gains by preventing protein synthesis. Our results suggest that aerobic exercise following FNDC5 muscle overexpression promotes osteoblast activity, which may be partially caused by muscle-derived FNDC5 secretion. In addition, OCN was necessary for muscle adaptation in both aerobic and resistance exercises.
骨骼肌和骨骼相互作用,以维持其结构和功能。体育锻炼是维持肌肉和骨骼功能的最有效、最易操作的策略;然而,运动诱导的可溶性因子之间的相互作用仍然难以捉摸。我们的研究旨在通过研究(1)肌动蛋白对骨骼的影响和(2)骨钙素(OCN)对骨骼肌的影响,确定运动诱导的肌肉和骨骼之间的相互作用。为了了解运动诱导的肌动蛋白对骨骼的影响,我们利用肌肉特异性肌动蛋白过表达模型,研究了有氧运动中 FNDC5 和阻力运动中 IGF-1 的影响。为了研究 OCN 对肌肉的影响,我们在小鼠长期运动期间腹腔注射了 OCN 中和抗体。结果表明,在肌肉特异性过表达组中,有氧运动往往会增加血清中附着于 FNDC5 的 HA 标记蛋白。此外,只有在HA/FNDC5过表达的有氧运动后,成骨细胞活化才会增加。IGF-1/HA过表达后,阻力运动不会改变循环中的HA-tag(肌肉来源的IGF-1)和骨代谢。在 OCN 研究中,有氧运动通过恢复肌糖原含量提高了耐力能力;然而,OCN 中和后,肌糖原含量恢复到基线水平。阻力运动后,OCN抑制通过阻止蛋白质合成来抑制肌肉肥大和力量增加。我们的研究结果表明,肌肉过表达 FNDC5 后进行有氧运动可促进成骨细胞的活性,这可能部分是由肌肉衍生的 FNDC5 分泌引起的。此外,有氧运动和阻力运动中的肌肉适应都离不开 OCN。
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引用次数: 0
Assessment of trabecular bone score (TBS) in the prediction of vertebral fracture in postmenopausal osteoporosis 评估骨小梁评分(TBS)在预测绝经后骨质疏松症患者脊椎骨折中的作用。
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-30 DOI: 10.1016/j.bone.2024.117307
The study aimed to evaluate the role of trabecular bone score (TBS) as determinant in the risk for vertebral fracture (VF) and define specific TBS threshold/s in women with postmenopausal osteoporosis.
We studied 107 women with postmenopausal osteoporosis characterized by L1-L4 T-score ≤ −3.0 with (group 1) and without (group 2) VF, or L1-L4 T-score ≤ −1.0 and ≥ −2.4 and multiple vertebral fractures (VF) (group 3). We assessed 30 postmenopausal women with L1-L4 T-score ≤ −1.0 and ≥ −2.4 and no VF as controls (group 4). We measured L1-L4, femoral neck and total hip areal bone mineral density (aBMD) by dual X-ray absorptiometry (DXA) (QDR 4500; Hologic, Waltham, MA) and calculated TBS from de-identified DXA L1-L4 scans by the TBS iNsight software (Medimaps, Geneva, Switzerland). The assessment of VF was performed by means of anteroposterior and left lateral standardized radiographs of the thoracic and lumbar spine. We calculated the FRAX® value in all subjects for the assessment of 10-year fracture risk for major and hip fractures.
Forty-two subjects with L1-L4 T-score ≤ −3.0 had at least one VF (group 1), while 41 have no VF (group 2). Twenty-four subjects had L1-L4 T-score ≤ −1.0 and ≥ −2.4 and at least 3 VF (group 3). We observed significantly lower TBS values in group 1 and group 3 compared to group 2 (p < 0.001) and group 4 (p < 0.05). L1-L4 aBMD and TBS values were not significantly associated in all groups. Interestingly, TBS values were independently associated with the presence of VF (log odds ratio − 8, p < 0.001) but not with the number of VF by the stepwise regression analysis. Furthermore, when we applied the cut-off value of TBS associated with degraded microarchitecture and elevated fracture risk (< 1.23), only 52 % of the subjects had VF. The cut-off value of TBS below which VF could be predicted was calculated by the receiver operating characteristic curve analysis and was 1.13.
Our study demonstrates an independent association between altered trabecular microarchitecture, assessed by TBS, and the occurrence of VF in postmenopausal women with osteoporosis. This association is significant for values of TBS lower than those reported by population-based studies. Cut-off values of TBS need further evaluation by specifically designed studies assessing disease- specific thresholds for fracture risk.
本研究旨在评估骨小梁评分(TBS)在椎体骨折(VF)风险中的决定性作用,并确定绝经后骨质疏松症妇女的特定 TBS 临界值/s。我们对 107 名绝经后骨质疏松症妇女进行了研究,这些妇女的特征是 L1-L4 T 评分≤-3.0 且有 VF(第 1 组)和无 VF(第 2 组),或 L1-L4 T 评分≤-1.0 且≥-2.4 且有多处椎体骨折(VF)(第 3 组)。我们评估了 30 名 L1-L4 T 评分≤-1.0 和≥-2.4 且无 VF 的绝经后妇女作为对照组(第 4 组)。我们通过双 X 射线吸收仪(DXA)(QDR 4500;Hologic,马萨诸塞州沃尔瑟姆)测量 L1-L4、股骨颈和全髋骨矿物质密度(aBMD),并使用 TBS iNsight 软件(Medimaps,瑞士日内瓦)从去标识化的 DXA L1-L4 扫描中计算 TBS。通过胸椎和腰椎的前正位和左外侧标准化X光片对VF进行评估。我们计算了所有受试者的 FRAX® 值,以评估重大骨折和髋部骨折的 10 年骨折风险。42 名 L1-L4 T 评分≤-3.0 的受试者至少有一个 VF(第 1 组),41 名没有 VF(第 2 组)。24 名受试者的 L1-L4 T 评分≤-1.0 和≥-2.4,且至少有 3 个 VF(第 3 组)。我们观察到,与第 2 组相比,第 1 组和第 3 组的 TBS 值明显较低(p
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引用次数: 0
Letter to the editor: “Exposure to low humidex increases the risk of hip fracture admissions in a subtropical coastal Chinese city” 致编辑的信:"中国亚热带沿海城市低湿环境增加髋部骨折入院风险"。
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-29 DOI: 10.1016/j.bone.2024.117311
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引用次数: 0
Neutrophils inhibit bone formation by directly contacting osteoblasts and suppressing osteogenic differentiation 中性粒细胞通过直接接触成骨细胞和抑制成骨分化来抑制骨形成。
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-28 DOI: 10.1016/j.bone.2024.117310
Neutrophils have been extensively studied for their critical roles in supporting immune defense mechanisms, initiating bone regeneration, and promoting angiogenesis. Nonetheless, the influence of neutrophils on physiological conditions, particularly in the context of bone development, remains incompletely understood. In this study, we examined the effects of non-inflammatory neutrophils on bone physiology by depleting Ly6G+ neutrophils and inducing neutropenia through myelosuppression. Our results demonstrated a notable increase in bone mass and a decrease in the bone marrow cavity upon depletion of the neutrophils. These effects were attributed to the direct interaction between neutrophils and osteoblasts, independent of reduced secretion of typical inflammatory cytokines or diminished osteoclast differentiation. This observation suggests a non-inflammatory function of neutrophils within the endosteal microenvironment, where they regulate osteogenic differentiation to preserve optimal bone mass, shape healthy three-dimensional bone trabecular structures, and create ample space for hematopoietic niche development.
中性粒细胞在支持免疫防御机制、启动骨再生和促进血管生成方面发挥着至关重要的作用,因此人们对其进行了广泛的研究。然而,中性粒细胞对生理状况的影响,尤其是对骨平衡的影响,仍未得到充分了解。在这项研究中,我们通过消耗 Ly6G+ 中性粒细胞并通过骨髓抑制诱导中性粒细胞减少,研究了非炎症性中性粒细胞对骨生理的影响。我们的研究结果表明,消耗成熟的中性粒细胞后,骨量明显增加,骨髓腔缩小。这种效应归因于中性粒细胞与成骨细胞之间的直接相互作用,与典型炎症细胞因子分泌减少或破骨细胞分化减弱无关。这一观察结果表明,中性粒细胞在骨内膜微环境中具有非炎症性功能,它们能调节成骨分化以保持最佳骨量,塑造健康的三维骨小梁结构,并为造血龛发育创造充足的空间。
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引用次数: 0
Histomorphometric parameters of iliac bone in healthy individuals: Systematic review and meta-analysis 健康人髂骨的组织形态计量参数:系统回顾和荟萃分析。
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-28 DOI: 10.1016/j.bone.2024.117309
Despite its invasive character, bone biopsy followed by histomorphometry remains the gold standard for diagnosing and classifying many metabolic bone diseases. However, the interpretation of histomorphometric parameters requires comparison with average values obtained from a proper control group, which are only available for some populations, and reference standards still need to be published. Therefore, our objective was to estimate average values for bone histomorphometric parameters overall, by age, gender, and race (White and Black) categories of healthy adult individuals, based on a systematic review and meta-analysis of clinical studies. Relevant studies published in English with available results until December 2020 were identified by PubMed (Medline) search and consulting experts in the field. Out of 447 potentially relevant studies, 37 met the inclusion criteria. Meta-analysis using fixed-effects models was used to pool mean estimates and 95% confidence intervals (CI) for 16 bone histomorphometry parameters.
An age-by-gender trend was observed in most histomorphometry parameters. The mean estimates of bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N) decreased. In contrast, trabecular separation (Tb.Sp) increased from the youngest to the oldest age categories in both genders. Osteoblast surface (Ob.S/BS) and osteoclast surface (Oc.S/BS) decreased across all age categories in males. Mineralizing surface (MS/BS) increased from the youngest to the oldest age categories in females, while mineralization lag time (Mlt) increased in both genders. Furthermore, gender and race had a significant effect on several histomorphometry parameters.
In conclusion, this meta-analysis provided mean estimates for normal values of histomorphometric parameters that clinicians may use when evaluating bone biopsies in patients. This enables the direct comparison of patients' histomorphometric values with the suitable reference group regarding age, gender, and race.
尽管骨活检具有侵入性,但其后的组织形态测量仍是诊断和分类许多代谢性骨病的黄金标准。然而,组织形态测量参数的解释需要与适当的对照组获得的平均值进行比较,而对照组只适用于某些人群,参考标准仍有待公布。因此,我们的目标是在对临床研究进行系统回顾和荟萃分析的基础上,按照健康成年人的年龄、性别和种族(白人和黑人)类别,估算出骨组织形态测量参数的总体平均值。通过 PubMed(Medline)检索和咨询该领域的专家,确定了在 2020 年 12 月之前发表的相关英文研究。在 447 项可能相关的研究中,有 37 项符合纳入标准。采用固定效应模型进行元分析,汇集了16个骨骼组织形态测量参数的平均估计值和95%置信区间(CI)。在大多数组织形态测量参数中都观察到了按性别划分的年龄趋势。骨体积/组织容积(BV/TV)、骨小梁厚度(Tb.Th)和骨小梁数量(Tb.N)的平均估计值有所下降。相比之下,从最年轻到最年长的年龄组别中,男女两性的骨小梁分离度(Tb.Sp)都有所增加。男性的成骨细胞表面(Ob.S/BS)和破骨细胞表面(Oc.S/BS)在所有年龄段都有所下降。女性的矿化表面(MS/BS)从最小年龄段到最大年龄段都有所增加,而矿化滞后时间(Mlt)在男女两性中都有所增加。此外,性别和种族对几个组织形态测量参数也有显著影响。总之,这项荟萃分析提供了组织形态计量参数正常值的平均估计值,临床医生在评估患者的骨活检结果时可以使用这些参数。这样就可以将患者的组织形态测量值与年龄、性别和种族方面的合适参照组进行直接比较。
{"title":"Histomorphometric parameters of iliac bone in healthy individuals: Systematic review and meta-analysis","authors":"","doi":"10.1016/j.bone.2024.117309","DOIUrl":"10.1016/j.bone.2024.117309","url":null,"abstract":"<div><div>Despite its invasive character, bone biopsy followed by histomorphometry remains the gold standard for diagnosing and classifying many metabolic bone diseases. However, the interpretation of histomorphometric parameters requires comparison with average values obtained from a proper control group, which are only available for some populations, and reference standards still need to be published. Therefore, our objective was to estimate average values for bone histomorphometric parameters overall, by age, gender, and race (White and Black) categories of healthy adult individuals, based on a systematic review and meta-analysis of clinical studies. Relevant studies published in English with available results until December 2020 were identified by PubMed (Medline) search and consulting experts in the field. Out of 447 potentially relevant studies, 37 met the inclusion criteria. Meta-analysis using fixed-effects models was used to pool mean estimates and 95% confidence intervals (CI) for 16 bone histomorphometry parameters.</div><div>An age-by-gender trend was observed in most histomorphometry parameters. The mean estimates of bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N) decreased. In contrast, trabecular separation (Tb.Sp) increased from the youngest to the oldest age categories in both genders. Osteoblast surface (Ob.S/BS) and osteoclast surface (Oc.S/BS) decreased across all age categories in males. Mineralizing surface (MS/BS) increased from the youngest to the oldest age categories in females, while mineralization lag time (Mlt) increased in both genders. Furthermore, gender and race had a significant effect on several histomorphometry parameters.</div><div>In conclusion, this meta-analysis provided mean estimates for normal values of histomorphometric parameters that clinicians may use when evaluating bone biopsies in patients. This enables the direct comparison of patients' histomorphometric values with the suitable reference group regarding age, gender, and race.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone properties in persons with type 1 diabetes and healthy controls – A cross-sectional study 1 型糖尿病患者和健康对照组的骨骼特性 - 一项横断面研究
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-28 DOI: 10.1016/j.bone.2024.117306
<div><h3>Background</h3><div>The risk of fractures is increased in persons with type 1 diabetes (T1D) and assessment of bone health has been included in the 2024 updated Standards of Care by The American Diabetes Association (ADA). Previous studies have found that in T1D bone metabolism, mineral content, microstructure, and strength diverge from that of persons without diabetes. However, a clear description of a T1D bone phenotype has not yet been established. We investigated bone mechanical properties and microstructure in T1D compared with healthy controls. For the potential future introduction of additional bone measures in the clinical fracture risk assessment, we aimed to assess any potential associations between various measures related to bone indices in subjects with T1D.</div></div><div><h3>Methods</h3><div>We studied human bone indices in a clinical cross-sectional setup including 111 persons with early-onset T1D and 37 sex- and age-matched control persons. Participants underwent hip and spine DXA scans for bone mineral density (BMD) of the femoral neck (FN), total hip (TH), and lumbar spine (LS), and TBS evaluation, microindentation of the tibial shaft for Bone Material Strength index (BMSi), and high-resolution periphery quantitative computed tomography (HRpQCT) of the distal radius and tibia for volumetric BMD (vBMD) and structural measures of trabecular and cortical bone. Results are reported as means with (standard deviation) or (95% confidence intervals (CI)), medians with [interquartile range], and differences are reported with (95% CI).</div></div><div><h3>Results</h3><div>The study included 148 persons aged 20 to 75 years with a median age of 43.2 years. The T1D group who had all been diagnosed with T1D before the age of 18 years demonstrated values of HbA1c ranging from 39 to 107 mmol/mol and a median HbA1c of 57 mmol/mol. The BMD did not differ between groups (the mean difference in FN-BMD was 0.026 g/cm<sup>2</sup> (−0.026; 0.079), <em>p</em> = 0.319) and the median BMSi was comparable in the two groups (79.2 [73.6; 83.8] in the T1D group compared with 77.9 [70.5, 86.1] in the control group). Total and trabecular vBMD (Tb.vBMD), cortical thickness (Ct.Th), and trabecular thickness (Tb.Th) of both radius and tibia were lower in participants with T1D. The mean Tb.vBMD at the radius was 143.6 (38.5) mg/cm<sup>3</sup> in the T1D group and 171.5 (37.7) mg/cm<sup>3</sup> in the control group, <em>p</em> < 0.001. The mean Ct. Th<sup>d</sup> of the radius was 0.739 mm (0.172) in the T1D group and 0.813 (0.188) in the control group, <em>p</em> = 0.044. Crude linear regressions revealed limited agreement between BMSi and Tb.vBMD (<em>p</em> = 0.010, r<sup>2</sup> = 0.040 at the radius and <em>p</em> = 0.008, r<sup>2</sup> = 0.040 at the tibia and between BMSi and the estimated failure load (FL) at the tibia (<em>p</em> < 0.001, r<sup>2</sup> = 0.090). There were no significant correlations between BMSi and Ct.Th. TBS correlated
背景1型糖尿病(T1D)患者发生骨折的风险增加,美国糖尿病协会(ADA)已将骨骼健康评估纳入2024年更新的护理标准。以往的研究发现,T1D 患者的骨代谢、矿物质含量、微观结构和强度与非糖尿病患者存在差异。然而,关于 T1D 骨表型的明确描述尚未确立。我们研究了 T1D 与健康对照组相比的骨机械性能和微观结构。为了将来在临床骨折风险评估中引入更多的骨骼测量指标,我们旨在评估与 T1D 患者骨骼指数相关的各种测量指标之间的潜在关联。方法我们在临床横断面设置中研究了人体骨骼指数,包括 111 名早发 T1D 患者和 37 名性别和年龄匹配的对照组患者。参与者接受了股骨颈(FN)、全髋(TH)和腰椎(LS)骨质密度(BMD)的髋关节和脊柱 DXA 扫描以及 TBS 评估、骨材料强度指数(BMSi)的胫骨轴显微压痕检查,以及桡骨远端和胫骨的高分辨率外围定量计算机断层扫描(HRpQCT),以测量骨体积密度(vBMD)以及骨小梁和皮质骨的结构。结果以带有(标准差)或(95% 置信区间 (CI))的均值、带有[四分位间范围]的中位数以及带有(95% CI)的差异进行报告。T1D 组患者均在 18 岁前被诊断出患有 T1D,他们的 HbA1c 值从 39 mmol/mol 到 107 mmol/mol 不等,中位 HbA1c 值为 57 mmol/mol。两组的 BMD 没有差异(FN-BMD 的平均差异为 0.026 g/cm2 (-0.026; 0.079),p = 0.319),两组的 BMSi 中位数相当(T1D 组为 79.2 [73.6; 83.8],对照组为 77.9 [70.5, 86.1])。T1D患者桡骨和胫骨的总vBMD和小梁vBMD(Tb.vBMD)、皮质厚度(Ct.Th)和小梁厚度(Tb.Th)均较低。T1D 组桡骨的平均 Tb.vBMD 为 143.6 (38.5) mg/cm3,对照组为 171.5 (37.7) mg/cm3,P < 0.001。桡骨的平均Ct.T1D组桡骨的平均Ct.Thd为0.739毫米(0.172),对照组为0.813(0.188),P = 0.044。粗线性回归显示,BMSi 和 Tb.vBMD 之间的一致性有限(桡骨为 p = 0.010,r2 = 0.040;胫骨为 p = 0.008,r2 = 0.040),BMSi 和胫骨的估计破坏负荷 (FL) 之间的一致性也有限(p < 0.001,r2 = 0.090)。BMSi 与 Ct.Th 之间无明显相关性。TBS与桡骨(p = 0.008,r2 = 0.044)和胫骨(p = 0.001,r2 = 0.069)的Tb.vBMD相关,与胫骨远端的估计FL相关(p = 0.038,r2 = 0.026)。与性别和年龄相匹配的健康对照组相比,我们发现总骨量和骨小梁vBMD降低,骨小梁和皮质厚度减少。这些结果表明,在达到峰值骨量之前首次发病的 T1D 会对骨微结构产生负面影响。在全髋骨密度(areal BMD)或全髋骨密度(BMSi)方面没有观察到差异。虽然总髋部 BMD 的变化反映了 vBMD 的一些变化,但 DXA 扫描并未捕捉到骨小梁骨矿物质密度的降低。因此,依靠 DXA 可能会低估骨折风险,因此有必要对 T1D 骨折风险评估进行进一步研究。
{"title":"Bone properties in persons with type 1 diabetes and healthy controls – A cross-sectional study","authors":"","doi":"10.1016/j.bone.2024.117306","DOIUrl":"10.1016/j.bone.2024.117306","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;The risk of fractures is increased in persons with type 1 diabetes (T1D) and assessment of bone health has been included in the 2024 updated Standards of Care by The American Diabetes Association (ADA). Previous studies have found that in T1D bone metabolism, mineral content, microstructure, and strength diverge from that of persons without diabetes. However, a clear description of a T1D bone phenotype has not yet been established. We investigated bone mechanical properties and microstructure in T1D compared with healthy controls. For the potential future introduction of additional bone measures in the clinical fracture risk assessment, we aimed to assess any potential associations between various measures related to bone indices in subjects with T1D.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;We studied human bone indices in a clinical cross-sectional setup including 111 persons with early-onset T1D and 37 sex- and age-matched control persons. Participants underwent hip and spine DXA scans for bone mineral density (BMD) of the femoral neck (FN), total hip (TH), and lumbar spine (LS), and TBS evaluation, microindentation of the tibial shaft for Bone Material Strength index (BMSi), and high-resolution periphery quantitative computed tomography (HRpQCT) of the distal radius and tibia for volumetric BMD (vBMD) and structural measures of trabecular and cortical bone. Results are reported as means with (standard deviation) or (95% confidence intervals (CI)), medians with [interquartile range], and differences are reported with (95% CI).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The study included 148 persons aged 20 to 75 years with a median age of 43.2 years. The T1D group who had all been diagnosed with T1D before the age of 18 years demonstrated values of HbA1c ranging from 39 to 107 mmol/mol and a median HbA1c of 57 mmol/mol. The BMD did not differ between groups (the mean difference in FN-BMD was 0.026 g/cm&lt;sup&gt;2&lt;/sup&gt; (−0.026; 0.079), &lt;em&gt;p&lt;/em&gt; = 0.319) and the median BMSi was comparable in the two groups (79.2 [73.6; 83.8] in the T1D group compared with 77.9 [70.5, 86.1] in the control group). Total and trabecular vBMD (Tb.vBMD), cortical thickness (Ct.Th), and trabecular thickness (Tb.Th) of both radius and tibia were lower in participants with T1D. The mean Tb.vBMD at the radius was 143.6 (38.5) mg/cm&lt;sup&gt;3&lt;/sup&gt; in the T1D group and 171.5 (37.7) mg/cm&lt;sup&gt;3&lt;/sup&gt; in the control group, &lt;em&gt;p&lt;/em&gt; &lt; 0.001. The mean Ct. Th&lt;sup&gt;d&lt;/sup&gt; of the radius was 0.739 mm (0.172) in the T1D group and 0.813 (0.188) in the control group, &lt;em&gt;p&lt;/em&gt; = 0.044. Crude linear regressions revealed limited agreement between BMSi and Tb.vBMD (&lt;em&gt;p&lt;/em&gt; = 0.010, r&lt;sup&gt;2&lt;/sup&gt; = 0.040 at the radius and &lt;em&gt;p&lt;/em&gt; = 0.008, r&lt;sup&gt;2&lt;/sup&gt; = 0.040 at the tibia and between BMSi and the estimated failure load (FL) at the tibia (&lt;em&gt;p&lt;/em&gt; &lt; 0.001, r&lt;sup&gt;2&lt;/sup&gt; = 0.090). There were no significant correlations between BMSi and Ct.Th. TBS correlated ","PeriodicalId":9301,"journal":{"name":"Bone","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “Small animal DXA instrument comparison and validation” [Bone 178 (2024) p 1–7, 116923] 小动物 DXA 仪器比较和验证"[Bone 178 (2024) p 1-7, 116923]的更正。
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-25 DOI: 10.1016/j.bone.2024.117298
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引用次数: 0
BMP3b regulates bone mass by inhibiting BMP signaling BMP3b 通过抑制 BMP 信号传导来调节骨量。
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-24 DOI: 10.1016/j.bone.2024.117303
Bone morphogenetic protein 3b (BMP3b), also known as growth differentiation factor 10 (GDF10), is a non-osteogenic BMP highly expressed in the skeleton. Although in vitro studies have shown that BMP3b suppresses osteoblast differentiation, the physiological role of BMP3b in regulating bone mass in vivo remains unknown. Here, we show that BMP3b deletion in mice leads to a high bone mass phenotype via an unexpected novel mechanism involving de-repression of canonical BMP/Smad signaling. BMP3b null mice were viable, and exhibited no significant difference in body size compared to wildtype control. Trabecular bone parameters assessed by histomorphometry and μCT, revealed a significant increase in bone volume and bone mineral density. Expression of osteoblast-differentiation genes were elevated in bone tissue of BMP3b null mice, whereas expression of osteoclast-related genes remained unchanged. Consistent with this, Bmp3b was highly expressed in osteoblasts relative to osteoclast cells. Ex-vivo culture of primary bone marrow mesenchymal stem cells (BMSCs) and primary bone marrow-derived osteoclasts revealed that inactivation of BMP3b enhances osteogenesis without affecting osteoclastogenesis. Mechanistically, we found that BMP3b suppressed BMP4-induced Smad1/5 phosphorylation and inhibited the activity of a BMP4-driven Id-1 luciferase reporter. Protein-protein interaction assays revealed that BMP3b competitively interfered with the association of BMP4 and BMP type I receptors. These findings suggest that BMP3b regulates bone mass by acting as a BMP receptor antagonist. Thus, maintenance of bone mass involves antagonism of canonical BMP/Smad signaling by a member of the BMP family.
骨形态发生蛋白 3b(BMP3b)又称生长分化因子 10(GDF10),是一种在骨骼中高度表达的非成骨性 BMP。虽然体外研究表明 BMP3b 可抑制成骨细胞分化,但 BMP3b 在体内调节骨量的生理作用仍不清楚。在这里,我们发现小鼠体内 BMP3b 的缺失会通过一种意想不到的新机制导致高骨量表型,这种新机制涉及去抑制典型 BMP/Smad 信号传导。BMP3b缺失小鼠存活率高,体型与野生型对照组相比无显著差异。通过组织形态学和μCT评估的骨小梁参数显示,骨量和骨矿物质密度显著增加。BMP3b无效小鼠骨组织中成骨细胞分化基因的表达升高,而破骨细胞相关基因的表达保持不变。与此相一致的是,相对于破骨细胞,Bmp3b在成骨细胞中的表达量较高。原代骨髓间充质干细胞(BMSCs)和原代骨髓破骨细胞的体内外培养显示,BMP3b失活可增强成骨过程,而不影响破骨细胞的生成。从机理上讲,我们发现BMP3b抑制了BMP4诱导的Smad1/5磷酸化,并抑制了BMP4驱动的Id-1荧光素酶报告物的活性。蛋白-蛋白相互作用测定显示,BMP3b 竞争性地干扰了 BMP4 和 BMP I 型受体的结合。这些发现表明,BMP3b 通过作为 BMP 受体拮抗剂来调节骨量。因此,骨量的维持涉及到 BMP 家族成员对典型 BMP/Smad 信号的拮抗作用。
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引用次数: 0
Single-cell transcriptomic resolution of osteogenesis during craniofacial morphogenesis 单细胞转录组解析颅面形态发生过程中的成骨过程
IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Pub Date : 2024-10-24 DOI: 10.1016/j.bone.2024.117297
Craniofacial morphogenesis depends on complex cell fate decisions during the differentiation of post-migratory cranial neural crest cells. Molecular mechanisms of cell differentiation of mesenchymal cells to developing bones, cartilage, teeth, tongue, and other craniofacial tissues are still poorly understood. We performed single-cell transcriptomic analysis of craniofacial mesenchymal cells derived from cranial NCCs in mouse embryo. Using FACS sorting of Wnt1-Cre2 progeny, we carefully mapped the cell heterogeneity in the craniofacial region during the initial stages of cartilage and bone formation. Transcriptomic data and in vivo validations identified molecular determinants of major cell populations involved in the development of lower and upper jaw, teeth, tongue, dermis, or periocular mesenchyme. Single-cell transcriptomic analysis of Meis2-deficient mice revealed critical gene expression differences, including increased osteogenic and cell adhesion markers. This leads to affected mesenchymal cell differentiation and increased ossification, resulting in impaired bone, cartilage, and tongue formation.
颅面形态发生取决于颅神经嵴后迁移细胞分化过程中复杂的细胞命运决定。间充质细胞分化为发育中的骨骼、软骨、牙齿、舌头和其他颅面组织的细胞分化分子机制仍鲜为人知。我们对源自小鼠胚胎颅骨神经细胞的颅面间充质细胞进行了单细胞转录组分析。利用 FACS 分选 Wnt1-Cre2 后代,我们仔细绘制了软骨和骨形成初期颅面区域细胞异质性的图谱。转录组数据和体内验证确定了参与上下颌骨、牙齿、舌头、真皮或眼周间质发育的主要细胞群的分子决定因素。对Meis2缺陷小鼠进行的单细胞转录组分析发现了关键的基因表达差异,包括成骨和细胞粘附标记物的增加。这导致间充质细胞分化受到影响,骨化增加,从而影响骨骼、软骨和舌头的形成。
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Bone
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