Genetic Characterization of Antibiotic-Resistant Staphylococcus spp. and Mammaliicoccus sciuri from Healthy Humans and Poultry in Nigeria.

IF 4.3 2区医学 Q1 INFECTIOUS DISEASES Antibiotics-Basel Pub Date : 2024-08-05 DOI:10.3390/antibiotics13080733

Christiana Jesumirhewe, Tolulope Oluwadamilola Odufuye, Juliana Ukinebo Ariri, Amdallat Arike Adebiyi, Amina Tanko Sanusi, Anna Stöger, Beatriz Daza-Prieto, Franz Allerberger, Adriana Cabal-Rosel, Werner Ruppitsch

{"title":"Genetic Characterization of Antibiotic-Resistant Staphylococcus spp. and Mammaliicoccus sciuri from Healthy Humans and Poultry in Nigeria.","authors":"Christiana Jesumirhewe, Tolulope Oluwadamilola Odufuye, Juliana Ukinebo Ariri, Amdallat Arike Adebiyi, Amina Tanko Sanusi, Anna Stöger, Beatriz Daza-Prieto, Franz Allerberger, Adriana Cabal-Rosel, Werner Ruppitsch","doi":"10.3390/antibiotics13080733","DOIUrl":null,"url":null,"abstract":"Staphylococcus spp. poses a significant threat to human and animal health due to their capacity to cause a wide range of infections in both. In this study, resistance genes conferring antibiotic resistance in Staphylococcus spp. and Mammaliicoccus sciuri isolates from humans and poultry in Edo state, Nigeria, were investigated. In April 2017, 61 Staphylococcus spp. isolates were obtained from urine, wounds, nasal and chicken fecal samples. Species identification was carried out by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Antimicrobial susceptibility testing was performed using the Kirby-Bauer method for 16 antibiotics. Whole-genome sequencing was used for characterization of the isolates. The 61 investigated isolates included Staphylococcus aureus, S. arlettae, M. sciuri, S. haemolyticus, and S. epidermidis. A total of 47 isolates (77%) belonged to human samples and 14 (23%) isolates were collected from poultry samples. All were phenotypically resistant to at least three antimicrobial(s). Multiple resistance determinants were detected in the human and poultry isolates analyzed. Phylogenetic analysis revealed close relatedness among the isolates within each species for S. arlettae, M. sciuri, and S. haemolyticus, respectively. This study delivered comprehensive genomic insights into antibiotic-resistant Staphylococcus species and M. sciuri isolates from human and poultry sources in Edo state, Nigeria, from a One Health perspective.","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11350800/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics13080733","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Staphylococcus spp. poses a significant threat to human and animal health due to their capacity to cause a wide range of infections in both. In this study, resistance genes conferring antibiotic resistance in Staphylococcus spp. and Mammaliicoccus sciuri isolates from humans and poultry in Edo state, Nigeria, were investigated. In April 2017, 61 Staphylococcus spp. isolates were obtained from urine, wounds, nasal and chicken fecal samples. Species identification was carried out by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Antimicrobial susceptibility testing was performed using the Kirby-Bauer method for 16 antibiotics. Whole-genome sequencing was used for characterization of the isolates. The 61 investigated isolates included Staphylococcus aureus, S. arlettae, M. sciuri, S. haemolyticus, and S. epidermidis. A total of 47 isolates (77%) belonged to human samples and 14 (23%) isolates were collected from poultry samples. All were phenotypically resistant to at least three antimicrobial(s). Multiple resistance determinants were detected in the human and poultry isolates analyzed. Phylogenetic analysis revealed close relatedness among the isolates within each species for S. arlettae, M. sciuri, and S. haemolyticus, respectively. This study delivered comprehensive genomic insights into antibiotic-resistant Staphylococcus species and M. sciuri isolates from human and poultry sources in Edo state, Nigeria, from a One Health perspective.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

尼日利亚健康人和家禽中的抗生素耐药葡萄球菌属和 Mammaliicoccus sciuri 的遗传特征。

葡萄球菌可引起多种感染，对人类和动物健康构成严重威胁。本研究调查了尼日利亚埃多州从人类和家禽中分离出的葡萄球菌属和Mammaliicoccus sciuri中赋予抗生素耐药性的耐药基因。2017 年 4 月，从尿液、伤口、鼻腔和鸡粪便样本中获得了 61 株葡萄球菌属分离物。通过基质辅助激光解吸电离飞行时间质谱法进行了菌种鉴定。采用柯比-鲍尔法对 16 种抗生素进行了抗菌药敏感性测试。全基因组测序用于鉴定分离物的特征。调查的 61 个分离菌株包括金黄色葡萄球菌、阿雷泰葡萄球菌、M. sciuri 葡萄球菌、溶血性葡萄球菌和表皮葡萄球菌。共有 47 个分离菌株（77%）来自人类样本，14 个分离菌株（23%）来自家禽样本。所有分离物均对至少三种抗菌药具有表型耐药性。在分析的人类和家禽分离物中检测到多种耐药性决定因子。系统发育分析表明，在每个物种中，阿雷特氏菌、苏合香杆菌和溶血性链球菌分离物之间的亲缘关系很近。这项研究从 "一体健康 "的角度，对尼日利亚埃多州从人类和家禽中分离出的耐抗生素葡萄球菌和M.sciuri进行了全面的基因组研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.