Multi-locus sequence typing and genetic diversity of antibiotic-resistant genes and virulence-associated genes in Burkholderia pseudomallei: Insights from whole genome sequencing of animal and environmental isolates in Thailand

IF 2.4 2区农林科学 Q3 MICROBIOLOGY Veterinary microbiology Pub Date : 2024-08-22 DOI:10.1016/j.vetmic.2024.110236

Sa-ngob Laklaeng , Jirarat Songsri , Sueptrakool Wisessombat , Wanida Mala , Preeda Phothaworn , Wilaiwan Senghoi , Manit Nuinoon , Sithichoke Tangphatsornruang , Teumpong Wongtawan , Orachun Hayakijkosol , Anusak Kerdsin , Wiyada Kwanhian Klangbud

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Abstract

Burkholderia pseudomallei is a Gram-negative bacillus and the etiological agent of melioidosis in humans and animals. The disease is highly endemic in northern Australia and Southeast Asia. Comprehensive genomic data are essential for understanding the bacteria's dissemination and genetic relationships among strains from different geographical regions. In this study, we conducted antimicrobial susceptibility testing and whole-genome sequencing of 54 B. pseudomallei isolates obtained from environmental and animal sources in southern Thailand between 2011 and 2018. Their genomics were determined of antibiotic-resistant genes (ARGs), virulence-associated genes, mobile genetic elements (MGEs), sequence types (STs), and single nucleotide polymorphisms (SNPs) to evaluate their epidemiological relatedness. Remarkably, all 54 isolates displayed sensitivity to antimicrobial agents typically used for melioidosis treatment. We identified nine distinct sequence types: ST392, ST51, ST409, ST508, ST376, ST1721, ST389, ST395, and ST289. Oxacillinase genes and the resistance nodulation family of efflux pumps (RND) were identified as contributors to antimicrobial resistance. Phylogenetic analysis demonstrated close genetic relations with other strains isolated from Southeast Asia. Furthermore, 172 virulence-associated genes were identified among the isolates, suggesting variations in clinical presentations. These findings underscore the importance of ongoing molecular genetic surveillance of B. pseudomallei for effective healthcare management and reducing melioidosis mortality.

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假丝酵母伯克霍尔德氏菌中抗生素耐药基因和毒力相关基因的多焦点序列分型和遗传多样性：泰国动物和环境分离物全基因组测序的启示

伯克霍尔德氏假丝酵母菌是一种革兰氏阴性杆菌，是人类和动物美拉德氏病的病原体。这种疾病在澳大利亚北部和东南亚高度流行。全面的基因组数据对于了解该细菌的传播和不同地理区域菌株之间的遗传关系至关重要。在这项研究中，我们对 2011 年至 2018 年期间从泰国南部环境和动物来源获得的 54 株假马来线虫病分离菌进行了抗菌药敏感性测试和全基因组测序。我们测定了它们的抗生素耐药基因（ARGs）、毒力相关基因、移动遗传因子（MGEs）、序列类型（STs）和单核苷酸多态性（SNPs）的基因组学，以评估它们的流行病学相关性。值得注意的是，所有 54 个分离株都对通常用于治疗类鼻疽的抗菌药敏感。我们确定了九种不同的序列类型：ST392、ST51、ST409、ST508、ST376、ST1721、ST389、ST395 和 ST289。经鉴定，氧青霉素酶基因和外排泵抗性结节家族（RND）是导致抗菌素耐药性的因素。系统发育分析表明，该菌株与从东南亚分离的其他菌株有着密切的遗传关系。此外，在分离株中还发现了 172 个毒力相关基因，表明临床表现存在差异。这些发现强调了对假丝酵母菌进行持续的分子遗传监测对于有效的医疗管理和降低类鼻疽死亡率的重要性。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.