Lei Xu , Yan Zhou , Congrong Miao , Hong Chen , Jianwei Zhang , Haoyu Qian , Pengfu Hou , Yanfeng Ding , Zhenghui Liu , Weiwei Li , Songhan Wang , Yu Jiang , Ganghua Li
{"title":"Long-term straw return increases fungal residual contribution to soil microaggregate nitrogen pool: An eco-enzymatic stoichiometric study","authors":"Lei Xu , Yan Zhou , Congrong Miao , Hong Chen , Jianwei Zhang , Haoyu Qian , Pengfu Hou , Yanfeng Ding , Zhenghui Liu , Weiwei Li , Songhan Wang , Yu Jiang , Ganghua Li","doi":"10.1016/j.still.2024.106278","DOIUrl":null,"url":null,"abstract":"<div><p>Straw return is a widespread agricultural practice for improving cropland nitrogen (N) stocks. However, the contribution of microbial N to the soil aggregate N pool and the underlying microbial metabolic regulation mechanisms remain uncertain. This study was based on a 13-year field experiment with rice (<em>Oryza sativa</em> L.) and wheat (<em>Triticum aestivum</em> L.) rotation, using only a chemical fertilizer alone (CF) as the control. We analyzed the effects of the chemical fertilizer combined with (CS, 9500 kg ha<sup>−1</sup> y<sup>−1</sup>) and wheat (4000 kg ha<sup>−1</sup> y<sup>−1</sup>) straw on microbial derived-N, microbial carbon (C) and N limitations. We also assessed microbial N use efficiency (NUE) in various aggregates of ferric lixisols (0<em><strong>–</strong></em>20 cm). Rotary tillage reached a depth of 20 cm. The CS significantly increased microbial-derived N concentrations in soil aggregates and enhanced the contribution of fungal residual N to the N pool in aggregates < 0.25 mm, but did not affect those > 0.25 mm. Conversely, the bacterial contribution to the N pool was not affected by CS. Meanwhile, CS significantly increased the soil organic C and microbial biomass in the aggregates. The results of our eco-enzymatic stoichiometric model revealed that the CS significantly alleviated microbial C limitations and increased microbial NUE in soil aggregates. Structural equation modeling further revealed that the microbial biomass and soil organic C contents are key drivers of the microbial C limitation. The increased contribution of fungal residual N to the N pools in < aggregates 0.25 mm was attributed to improved microbial NUE resulting from the straw, without altering net N mineralization rates or β-1,4-N-acetylglucosidase activity. Our findings suggest that straw return promotes microbial-derived N production and sequestration by alleviating microbial C limitation. The strategies governing these microbial-derived N responses in aggregates to straw return might vary. This might be valuable for designing cropland management practices to improve N storage.</p></div>","PeriodicalId":49503,"journal":{"name":"Soil & Tillage Research","volume":"244 ","pages":"Article 106278"},"PeriodicalIF":6.1000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Soil & Tillage Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167198724002794","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Straw return is a widespread agricultural practice for improving cropland nitrogen (N) stocks. However, the contribution of microbial N to the soil aggregate N pool and the underlying microbial metabolic regulation mechanisms remain uncertain. This study was based on a 13-year field experiment with rice (Oryza sativa L.) and wheat (Triticum aestivum L.) rotation, using only a chemical fertilizer alone (CF) as the control. We analyzed the effects of the chemical fertilizer combined with (CS, 9500 kg ha−1 y−1) and wheat (4000 kg ha−1 y−1) straw on microbial derived-N, microbial carbon (C) and N limitations. We also assessed microbial N use efficiency (NUE) in various aggregates of ferric lixisols (0–20 cm). Rotary tillage reached a depth of 20 cm. The CS significantly increased microbial-derived N concentrations in soil aggregates and enhanced the contribution of fungal residual N to the N pool in aggregates < 0.25 mm, but did not affect those > 0.25 mm. Conversely, the bacterial contribution to the N pool was not affected by CS. Meanwhile, CS significantly increased the soil organic C and microbial biomass in the aggregates. The results of our eco-enzymatic stoichiometric model revealed that the CS significantly alleviated microbial C limitations and increased microbial NUE in soil aggregates. Structural equation modeling further revealed that the microbial biomass and soil organic C contents are key drivers of the microbial C limitation. The increased contribution of fungal residual N to the N pools in < aggregates 0.25 mm was attributed to improved microbial NUE resulting from the straw, without altering net N mineralization rates or β-1,4-N-acetylglucosidase activity. Our findings suggest that straw return promotes microbial-derived N production and sequestration by alleviating microbial C limitation. The strategies governing these microbial-derived N responses in aggregates to straw return might vary. This might be valuable for designing cropland management practices to improve N storage.
期刊介绍:
Soil & Tillage Research examines the physical, chemical and biological changes in the soil caused by tillage and field traffic. Manuscripts will be considered on aspects of soil science, physics, technology, mechanization and applied engineering for a sustainable balance among productivity, environmental quality and profitability. The following are examples of suitable topics within the scope of the journal of Soil and Tillage Research:
The agricultural and biosystems engineering associated with tillage (including no-tillage, reduced-tillage and direct drilling), irrigation and drainage, crops and crop rotations, fertilization, rehabilitation of mine spoils and processes used to modify soils. Soil change effects on establishment and yield of crops, growth of plants and roots, structure and erosion of soil, cycling of carbon and nutrients, greenhouse gas emissions, leaching, runoff and other processes that affect environmental quality. Characterization or modeling of tillage and field traffic responses, soil, climate, or topographic effects, soil deformation processes, tillage tools, traction devices, energy requirements, economics, surface and subsurface water quality effects, tillage effects on weed, pest and disease control, and their interactions.