Validation of the diagnostic accuracy of the 3M™ Petrifilm™ coliform and aerobic count plates to measure colostrum bacterial contamination on Scottish dairy farms

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES Veterinary journal Pub Date : 2024-08-30 DOI:10.1016/j.tvjl.2024.106230
Tracy Anderson , Alexandra Haggerty , Emily Silva , Colin Mason , David Bell , Katharine S. Denholm
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Abstract

A calf should receive 10–12 % of its bodyweight in high quality colostrum ( >50 g/L immunoglobulin) shortly after birth in order to confer passive immunity, with calves ideally receiving their first feed of colostrum in the first 1–2 hours of life (Godden et al., 2019). It is recommended (not validated) that total counts for bacteria and coliforms in colostrum should not exceed 100,000 colony forming units per mL (CFU/mL) and 10,000 CFU/mL, respectively. This study was conducted between March and September 2023. Eighty-five colostrum samples from 5 commerical dairy farms in Dumfries and Galloway in Scotland were purposively selected. Samples were collected from various stages during the colostrum harvest, storage and feeding process to provide multiple samples with variable (low, medium and high) bacterial contamination. The objective of this study was to validate the diagnostic accuracy of the 3 M Petrifilm™ Coliform Count Plate (CCP) and Aerobic Count Plate (ACP) for colostrum bacteriology. The Petrifilm™ Aerobic Count Plates were compared to 5 % sheep blood agar (SBA) (total bacteria counts, TBC), and the Petrifilm™ Coliform Count Plates were compared to MacConkey plates (MAC) (total coliform counts, TCC) and test sensitivity and specificity were calculated.

Colostrum bacterial contamination was highly variable between farms (TBC median and interquartile range= 50,000CFU/mL and 546000CFU/mL; TCC median and interquartile range=100,750CFU/mL and 188,500CFU/mL). Overall correlation between the Petrifilms™ and reference plate measures was moderate (Spearman rho=0.38–0.47). The statistical accuracy of the Petrifilm™ systems and the test sensitivity were improved by lowering the bacterial cutpoint from 100,000CFU/mL to 91,000 CFU/mL for the aerobic counts plates and from 10,000CFU/mL to 1500 CFU/mL for the coliform count plates. The Petrifilm™ system could be used as a monitoring tool to provide quick and statistically accurate results for TBC and TCC but should not be seen as a comprehensive solution for poor colostrum hygiene.

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验证 3M™ Petrifilm™ 大肠菌群和需氧菌计数板测量苏格兰奶牛场初乳细菌污染的诊断准确性
犊牛出生后不久就应摄入占其体重 10-12% 的优质初乳(免疫球蛋白含量为 50 克/升),以获得被动免疫,犊牛最好在出生后 1-2 小时内摄入第一口初乳(Godden 等人,2019 年)。建议(未经验证)初乳中细菌和大肠菌群的总计数不应分别超过每毫升 100,000 菌落总数(CFU/mL)和每毫升 10,000 菌落总数(CFU/mL)。这项研究在 2023 年 3 月至 9 月期间进行。研究人员有目的地从苏格兰邓弗里斯和加洛韦的 5 个商业奶牛场选取了 85 份初乳样本。样品从牛初乳收获、储存和饲喂过程的不同阶段采集,以提供细菌污染程度不同(低、中、高)的多个样品。这项研究的目的是验证 3 M Petrifilm™ 大肠菌群计数板(CCP)和需氧菌计数板(ACP)对牛初乳细菌学诊断的准确性。将 Petrifilm™ 需氧菌计数板与 5 % 羊血琼脂(SBA)(细菌总数,TBC)进行比较,将 Petrifilm™ 大肠菌群计数板与麦康凯平板(MAC)(大肠菌群总数,TCC)进行比较,并计算检测灵敏度和特异性。不同牧场之间的初乳细菌污染差异很大(TBC 中位数和四分位数范围分别为 50,000CFU/mL 和 546,000CFU/mL;TCC 中位数和四分位数范围分别为 100,750CFU/mL 和 188,500CFU/mL )。Petrifilms™ 和参考平板测量值之间的总体相关性为中等(Spearman rho=0.38-0.47)。将需氧菌落计数平板的细菌切点从 100,000CFU/mL 降至 91,000 CFU/mL,将大肠菌群计数平板的细菌切点从 10,000CFU/mL 降至 1500 CFU/mL,可提高 Petrifilm™ 系统的统计准确性和测试灵敏度。Petrifilm™ 系统可用作监测工具,为 TBC 和 TCC 提供快速、统计准确的结果,但不应被视为解决初乳卫生问题的综合方案。
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来源期刊
Veterinary journal
Veterinary journal 农林科学-兽医学
CiteScore
4.10
自引率
4.50%
发文量
79
审稿时长
40 days
期刊介绍: The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.
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