The effects of winter cold on acute copper bioaccumulation and toxicity in brook char (Salvelinus fontinalis)

Abstract

Freshwater fish that are acutely exposed to copper (Cu) can experience disturbances of ion regulation and ammonia excretion. Temperature has been shown to affect Cu bioaccumulation and toxicity in fish, but the focus has largely been on warm temperature effects. Yet, acclimation of freshwater fish to near-freezing temperatures encountered during the winter of temperate regions can challenge fish condition and physiology, including ion regulation. Thus, temperate freshwater fish might be particularly sensitive to Cu in the winter. We investigated how winter cold affects acute Cu bioaccumulation and toxicity in juvenile brook char (Salvelinus fontinalis). Following gradual acclimation to cold temperature (-2 °C/week from 14 °C, then 4 weeks at 3 °C) vs. a warmer temperature around the species thermal optimum (14 °C for 9 weeks), and following a cold challenge (-3 °C/day from 14 °C, then 24 h at 3 °C) vs. a cold acclimation (-2 °C/week from 14 °C, then 13 weeks at 3 °C), we measured gill-Cu bioaccumulation, net fluxes of ammonia (NH₃), chloride (Cl⁻) and net and unidirectional fluxes of sodium (Na⁺) over a 30-h Cu exposure. Overall, winter cold did not appear to be challenging to brook char, as cold-acclimated fish had a higher fish condition and showed no sign of ion regulation impairment or increased Cu sensitivity. Contrary to our prediction, we found that Cu bioaccumulation over a 30-h Cu exposure was not significantly affected by acclimation temperature. Effects of temperature on Cu physiological effects were relatively limited (mainly on inhibition of Na⁺ influx and of NH₃ excretion), with slightly greater effects observed in 14 °C-acclimated fish.