Saline-alkali stress affects the accumulation of proanthocyanidins and sesquiterpenoids via the MYB5-ANR/TPS31 cascades in the rose petals

IF 8.7 1区农林科学 Q1 Agricultural and Biological Sciences Horticulture Research Pub Date : 2024-08-30 DOI:10.1093/hr/uhae243

Qiao Wang, Baoquan Du, Yujing Bai, Yan Chen, Feng Li, Jinzhe Du, Xiuwen Wu, Liping Yan, Yue Bai, Guohua Chai

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Abstract

Rose (Rosa rugosa) petals are rich in diverse secondary metabolites, which have important physiological functions as well as great economic values. Currently, it remains unclear how saline and/or alkaline stress(es) influence the accumulation of secondary metabolites in rose. In this study, we analyzed the transcriptome and metabolite profiles of rose petals under aline-alkali stress and uncovered the induction mechanism underlying major metabolites. Dramatic changes were observed in the expression of 1363 genes and the abundances of 196 metabolites in petals in response to saline-alkali stress. These differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) are mainly associated with flavonoid and terpenoid metabolism and the reconstruction of cell walls. Of them, TERPENE SYNTHASE 31 (TPS31) overexpression in tobacco leaves driven by its own promoter resulted in significant alterations in the levels of various terpenoids, which were differentially influenced by saline-alkali stress. An integrated analysis of metabolomic and transcriptomic data revealed a high correlation between the abundances of flavonoids/terpenoids and the expression of the transcription factor MYB5. MYB5 may orchestrate the biosynthesis of sesquiterpenoids and proanthocyanidins through direct regulation of TPS31 and ANR expression under aline-alkali stress. Our finding facilitates improving the bioactive substance accumulation of rose petals by metabolic engineering.

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盐碱胁迫通过 MYB5-ANR/TPS31 级联影响玫瑰花瓣中原花青素和倍半萜类化合物的积累

玫瑰（Rosa rugosa）花瓣富含多种次生代谢物，具有重要的生理功能和巨大的经济价值。目前，人们还不清楚盐胁迫和/或碱胁迫如何影响玫瑰次生代谢物的积累。本研究分析了碱-碱胁迫下玫瑰花瓣的转录组和代谢物谱，揭示了主要代谢物的诱导机制。在盐碱胁迫下，花瓣中 1363 个基因的表达和 196 种代谢物的丰度发生了巨大变化。这些差异表达基因（DEGs）和差异积累代谢物（DAMs）主要与黄酮类和萜类化合物的代谢以及细胞壁的重建有关。其中，TERPENE SYNTHASE 31（TPS31）在其自身启动子的驱动下在烟草叶片中的过表达导致了各种萜类化合物水平的显著变化，这些变化受盐碱胁迫的不同影响。对代谢组和转录组数据的综合分析表明，类黄酮/萜类化合物的丰度与转录因子 MYB5 的表达高度相关。在碱-碱胁迫下，MYB5可能通过直接调控TPS31和ANR的表达来协调倍半萜和原花青素的生物合成。我们的发现有助于通过代谢工程改善玫瑰花瓣生物活性物质的积累。

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来源期刊

Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

11.20

自引率

6.90%

发文量

367

审稿时长

20 weeks

期刊介绍： Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.