Decoding plant-induced transcriptomic variability and consistency in two related polyphagous mites differing in host ranges.

IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Ecology Pub Date : 2024-08-29 DOI:10.1111/mec.17521
Lei Chen, Li-Xue Guo, Xin-Yue Yu, Shi-Mei Huo, Ary A Hoffmann, Jia-Yi Zhou, Jing-Tao Sun, Xiao-Yue Hong
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Abstract

The diet breadth of generalist herbivores when compared to specialists tends to be associated with greater transcriptional plasticity. Here, we consider whether it may also contribute to variation in host range among two generalists with different levels of polyphagy. We examined two related polyphagous spider mites with different host ranges, Tetranychus urticae (1200 plants) and Tetranychus truncatus (90 plants). Data from multiple populations of both species domesticated on common beans and transferred to new plant hosts (cotton, cucumber, eggplant) were used to investigate transcriptional plasticity relative to population-based variation in gene expression. Compared to T. truncatus, T. urticae exhibited much higher transcriptional plasticity. Populations of this species also showed much more variable expression regulation in response to a plant host, particularly for genes related to detoxification, transport, and transcriptional factors. In response to the different plant hosts, both polyphagous species showed enriched processes of drug/xenobiotics metabolism, with T. urticae orchestrating a relatively broader array of biological pathways. Through co-expression network analysis, we identified gene modules associated with host plant response, revealing shared hub genes primarily involved in detoxification metabolism when both mites fed on the same plants. After silencing a shared hub CYP gene related to eggplant exposure, the performance of both species on the original bean host improved, but the fecundity of T. truncatus decreased when feeding on eggplant. The extensive transcriptomic variation shown by T. urticae might serve as a potential compensatory mechanism for a deficiency of hub genes in this species. This research points to nuanced differences in transcriptomic variability between generalist herbivores.

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解码寄主范围不同的两种相关多食螨的植物诱导转录组变异性和一致性。
与专性食草动物相比,通性食草动物的食性广度往往与更强的转录可塑性有关。在此,我们将考虑这是否也会导致两种具有不同多食性的食草动物在寄主范围上的差异。我们研究了寄主范围不同的两种相关多食性蜘蛛螨:Tetranychus urticae(1200 株)和 Tetranychus truncatus(90 株)。研究人员利用在普通豆类上驯化并转移到新植物寄主(棉花、黄瓜、茄子)上的这两个物种的多个种群的数据,研究了基因表达的转录可塑性与基于种群的变异之间的关系。与T. truncatus相比,T. urticae表现出更高的转录可塑性。该物种的种群对植物宿主的反应也表现出更多的表达调控变化,尤其是与解毒、运输和转录因子相关的基因。在对不同植物宿主的反应中,两种多食性物种都表现出丰富的药物/类毒素代谢过程,其中T. urticae协调了相对更广泛的生物通路。通过共表达网络分析,我们确定了与寄主植物反应相关的基因模块,揭示了当两种螨虫取食同一种植物时,主要参与解毒代谢的共享中枢基因。在沉默了一个与茄子接触相关的共享中枢 CYP 基因后,两种螨虫在原豆科寄主上的表现都有所改善,但在茄子上取食时,T. truncatus 的繁殖力下降。T.urticae表现出的广泛转录组变异可能是该物种缺乏枢纽基因的一种潜在补偿机制。这项研究指出了食草动物之间转录组变异的细微差别。
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来源期刊
Molecular Ecology
Molecular Ecology 生物-进化生物学
CiteScore
8.40
自引率
10.20%
发文量
472
审稿时长
1 months
期刊介绍: Molecular Ecology publishes papers that utilize molecular genetic techniques to address consequential questions in ecology, evolution, behaviour and conservation. Studies may employ neutral markers for inference about ecological and evolutionary processes or examine ecologically important genes and their products directly. We discourage papers that are primarily descriptive and are relevant only to the taxon being studied. Papers reporting on molecular marker development, molecular diagnostics, barcoding, or DNA taxonomy, or technical methods should be re-directed to our sister journal, Molecular Ecology Resources. Likewise, papers with a strongly applied focus should be submitted to Evolutionary Applications. Research areas of interest to Molecular Ecology include: * population structure and phylogeography * reproductive strategies * relatedness and kin selection * sex allocation * population genetic theory * analytical methods development * conservation genetics * speciation genetics * microbial biodiversity * evolutionary dynamics of QTLs * ecological interactions * molecular adaptation and environmental genomics * impact of genetically modified organisms
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