首页 > 最新文献

Molecular Ecology最新文献

英文 中文
Population Genetics and Invasion History of the European Starling Across Aotearoa New Zealand. 欧洲椋鸟在新西兰奥特亚罗亚的种群遗传学和入侵历史。
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-22 DOI: 10.1111/mec.17579
Bryan Thompson, Kamolphat Atsawawaranunt, Melissa C Nehmens, William S Pearman, E Owen Perkins, Pavel Pipek, Lee A Rollins, Hui Zhen Tan, Annabel Whibley, Anna W Santure, Katarina C Stuart

The expansion of human settlements over the past few centuries is responsible for an unprecedented number of invasive species introductions globally. An important component of biological invasion management is understanding how introduction history and postintroduction processes have jointly shaped present-day distributions and patterns of population structure, diversity and adaptation. One example of a successful invader is the European starling (Sturnus vulgaris), which was intentionally introduced to numerous countries in the 19th century, including Aotearoa New Zealand, where it has become firmly established. We used reduced representation sequencing to characterise the genetic population structure of the European starling in New Zealand, comparing it to that present in sampling locations in the native range and invasive Australian range. The population structure and genetic diversity patterns we found suggested restricted gene flow from the majority of New Zealand to the northmost sampling location (Auckland). We also profiled genetic bottlenecks and shared outlier genomic regions, which supported historical accounts of translocations between both Australian subpopulations and New Zealand, and provided evidence of which documented translocation events were more likely to have been successful. Using these results as well as historic demographic patterns, we demonstrate how genomic analysis complements even well-documented invasion histories to better understand invasion processes, with direct implications for understanding contemporary gene flow and informing invasion management.

过去几个世纪中,人类居住区的扩张造成了全球数量空前的入侵物种引进。生物入侵管理的一个重要组成部分是了解引入历史和引入后的过程如何共同塑造了今天的分布以及种群结构、多样性和适应性模式。欧洲椋鸟(Sturnus vulgaris)就是一个成功入侵的例子,它在 19 世纪被有意引入许多国家,包括新西兰,并在那里扎下了根。我们利用还原表示测序技术描述了新西兰欧洲椋鸟的遗传种群结构,并将其与原生地和入侵地澳大利亚的取样地点的遗传种群结构进行了比较。我们发现的种群结构和遗传多样性模式表明,从新西兰大部分地区到最北端取样地点(奥克兰)的基因流动受到了限制。我们还分析了基因瓶颈和共同的离群基因组区域,这支持了澳大利亚亚种群和新西兰之间的历史迁徙记载,并提供了证据,证明哪些记录在案的迁徙事件更有可能取得成功。利用这些结果以及历史人口统计模式,我们展示了基因组分析如何补充即使是有据可查的入侵历史,从而更好地了解入侵过程,这对了解当代基因流和入侵管理具有直接影响。
{"title":"Population Genetics and Invasion History of the European Starling Across Aotearoa New Zealand.","authors":"Bryan Thompson, Kamolphat Atsawawaranunt, Melissa C Nehmens, William S Pearman, E Owen Perkins, Pavel Pipek, Lee A Rollins, Hui Zhen Tan, Annabel Whibley, Anna W Santure, Katarina C Stuart","doi":"10.1111/mec.17579","DOIUrl":"https://doi.org/10.1111/mec.17579","url":null,"abstract":"<p><p>The expansion of human settlements over the past few centuries is responsible for an unprecedented number of invasive species introductions globally. An important component of biological invasion management is understanding how introduction history and postintroduction processes have jointly shaped present-day distributions and patterns of population structure, diversity and adaptation. One example of a successful invader is the European starling (Sturnus vulgaris), which was intentionally introduced to numerous countries in the 19th century, including Aotearoa New Zealand, where it has become firmly established. We used reduced representation sequencing to characterise the genetic population structure of the European starling in New Zealand, comparing it to that present in sampling locations in the native range and invasive Australian range. The population structure and genetic diversity patterns we found suggested restricted gene flow from the majority of New Zealand to the northmost sampling location (Auckland). We also profiled genetic bottlenecks and shared outlier genomic regions, which supported historical accounts of translocations between both Australian subpopulations and New Zealand, and provided evidence of which documented translocation events were more likely to have been successful. Using these results as well as historic demographic patterns, we demonstrate how genomic analysis complements even well-documented invasion histories to better understand invasion processes, with direct implications for understanding contemporary gene flow and informing invasion management.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17579"},"PeriodicalIF":4.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Early-Life Disruption of Gut Microbiota Has Opposing Effects on Parasite Resistance in Two Host Species. 肠道微生物群的早期破坏对两种宿主的寄生虫抵抗力具有相反的影响
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-22 DOI: 10.1111/mec.17595
Ashley C Love, Mia L Nahom, Jessica Gutierrez, Lauren Albert, Sarah A Knutie

Gut microbiota regulate multiple aspects of host health, including metabolism and the development of the immune system. However, we still know relatively little about how the gut microbiota influences host responses to parasitism in wild organisms, particularly whether host-microbiota interactions contribute to variation in parasitism across host species. The goal of this study was to determine the role of gut microbiota in shaping how birds respond to nest parasites and investigate whether this relationship varies between host species. Both eastern bluebirds (Sialia sialis) and tree swallows (Tachycineta bicolor) are parasitized by blow flies (Protocalliphora sialia), for which larval flies feed on nestlings' blood. We experimentally manipulated the gut microbiota of nestling bluebirds and tree swallows by dosing nestlings with an oral antibiotic or sterile water as a control. We then quantified nestling physiology (haemoglobin, glucose, parasite-specific IgY antibody levels), body morphometrics, and survival until fledging, as well as blow fly abundance and size. An experimental disruption of nestling gut microbiota increased parasite abundance in tree swallows, but decreased parasite abundance in bluebirds, which suggests that the disruption has opposing effects on resistance across host species. Furthermore, experimental treatment delayed parasite development and had variable effects on nestling body morphometrics and physiology across the two host species. Together, these results suggest that gut microbiota contribute to host differences in resistance to blow flies and can influence host-parasite interactions.

肠道微生物群调节宿主健康的多个方面,包括新陈代谢和免疫系统的发育。然而,对于肠道微生物群如何影响野生生物宿主对寄生虫的反应,尤其是宿主与微生物群之间的相互作用是否会导致宿主物种之间寄生虫的差异,我们仍然知之甚少。本研究的目的是确定肠道微生物群在影响鸟类对巢穴寄生虫的反应中的作用,并调查这种关系在宿主物种之间是否存在差异。东部蓝鸟(Sialia sialis)和树燕(Tachycineta bicolor)都被吹蝇(Protocalliphora sialia)寄生,吹蝇幼虫以雏鸟的血液为食。我们通过实验操纵了蓝鸟和树燕雏鸟的肠道微生物群,给雏鸟口服抗生素或以无菌水作为对照。然后,我们对雏鸟的生理机能(血红蛋白、葡萄糖、寄生虫特异性 IgY 抗体水平)、身体形态测量、羽化前的存活率以及吹蝇的数量和大小进行了量化。实验性破坏雏鸟肠道微生物群会增加寄生虫在树燕体内的数量,但会降低寄生虫在蓝鸟体内的数量,这表明破坏微生物群对不同宿主物种的抵抗力有相反的影响。此外,实验性处理延缓了寄生虫的发育,并对两种宿主的雏鸟身体形态和生理产生了不同的影响。这些结果表明,肠道微生物群会导致宿主对吹蝇的抵抗力差异,并影响宿主与寄生虫之间的相互作用。
{"title":"An Early-Life Disruption of Gut Microbiota Has Opposing Effects on Parasite Resistance in Two Host Species.","authors":"Ashley C Love, Mia L Nahom, Jessica Gutierrez, Lauren Albert, Sarah A Knutie","doi":"10.1111/mec.17595","DOIUrl":"https://doi.org/10.1111/mec.17595","url":null,"abstract":"<p><p>Gut microbiota regulate multiple aspects of host health, including metabolism and the development of the immune system. However, we still know relatively little about how the gut microbiota influences host responses to parasitism in wild organisms, particularly whether host-microbiota interactions contribute to variation in parasitism across host species. The goal of this study was to determine the role of gut microbiota in shaping how birds respond to nest parasites and investigate whether this relationship varies between host species. Both eastern bluebirds (Sialia sialis) and tree swallows (Tachycineta bicolor) are parasitized by blow flies (Protocalliphora sialia), for which larval flies feed on nestlings' blood. We experimentally manipulated the gut microbiota of nestling bluebirds and tree swallows by dosing nestlings with an oral antibiotic or sterile water as a control. We then quantified nestling physiology (haemoglobin, glucose, parasite-specific IgY antibody levels), body morphometrics, and survival until fledging, as well as blow fly abundance and size. An experimental disruption of nestling gut microbiota increased parasite abundance in tree swallows, but decreased parasite abundance in bluebirds, which suggests that the disruption has opposing effects on resistance across host species. Furthermore, experimental treatment delayed parasite development and had variable effects on nestling body morphometrics and physiology across the two host species. Together, these results suggest that gut microbiota contribute to host differences in resistance to blow flies and can influence host-parasite interactions.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17595"},"PeriodicalIF":4.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elevational Range Impacts Connectivity and Predicted Deme Sizes From Models of Habitat Suitability. 海拔高度影响连通性和栖息地适宜性模型预测的生态群落规模。
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-21 DOI: 10.1111/mec.17593
Connor M French, Roberta P Damasceno, Mariana M Vasconcellos, Miguel T Rodrigues, Ana C Carnaval, Michael J Hickerson

In integrative distributional, demographic and coalescent (iDDC) modelling, a critical component is the statistical relationship between habitat suitability and local population sizes. This study explores this relationship in two Enyalius lizard species from the Brazilian Atlantic Forest: the high-elevation E. iheringii and low-elevation E. catenatus and how this transformation affects spatiotemporal demographic inference. Most previous iDDC studies assumed a linear relationship, but this study hypothesises that the relationship may be nonlinear, especially for high-elevation species with broader environmental tolerances. We test two key hypotheses: (1) The habitat suitability to population size relationship is nonlinear for E. iheringii (high-elevation) and linear for E. catenatus (low-elevation); and (2) E. iheringii exhibits higher effective migration across populations than E. catenatus. Our findings provide clear support for hypothesis (2), but mixed support for hypothesis (1), with strong model support for a nonlinear transformation in the high-elevation E. iheringii and some (albeit weak) support for a nonlinear transformation also in E. catenatus. The iDDC models allow us to generate landscape-wide maps of predicted genetic diversity for both species, revealing that genetic diversity predictions for the high-elevation E. iheringii align with estimated patterns of historical range stability, whereas predictions for low-elevation E. catenatus are distinct from range-wide stability predictions. This research highlights the importance of accurately modelling the habitat suitability to population size relationship in iDDC studies, contributing to our understanding of species' demographic responses to environmental changes.

在综合分布、人口和聚合(iDDC)建模中,栖息地适宜性与当地种群数量之间的统计关系是一个关键组成部分。本研究探讨了巴西大西洋森林中两个Enyalius蜥蜴物种(高海拔的E. iheringii和低海拔的E. catenatus)的这种关系,以及这种转变如何影响时空人口推断。之前的大多数 iDDC 研究都假定两者之间存在线性关系,但本研究假设两者之间可能存在非线性关系,尤其是对环境耐受性更强的高海拔物种而言。我们检验了两个关键假设:(1)E. iheringii(高海拔)的栖息地适宜性与种群数量的关系是非线性的,而 E. catenatus(低海拔)的栖息地适宜性与种群数量的关系是线性的;(2)E. iheringii比 E. catenatus表现出更高的种群间有效迁移。我们的研究结果为假设(2)提供了明确的支持,但为假设(1)提供了不同的支持,高海拔 E. iheringii 的非线性变化得到了模型的有力支持,E. catenatus 的非线性变化也得到了一些支持(尽管很弱)。iDDC 模型允许我们生成这两个物种的全地貌遗传多样性预测图,揭示了高海拔 E. iheringii 的遗传多样性预测与历史分布区稳定性的估计模式一致,而低海拔 E. catenatus 的预测则与全分布区稳定性预测不同。这项研究强调了在 iDDC 研究中准确模拟栖息地适宜性与种群数量关系的重要性,有助于我们了解物种对环境变化的人口响应。
{"title":"Elevational Range Impacts Connectivity and Predicted Deme Sizes From Models of Habitat Suitability.","authors":"Connor M French, Roberta P Damasceno, Mariana M Vasconcellos, Miguel T Rodrigues, Ana C Carnaval, Michael J Hickerson","doi":"10.1111/mec.17593","DOIUrl":"https://doi.org/10.1111/mec.17593","url":null,"abstract":"<p><p>In integrative distributional, demographic and coalescent (iDDC) modelling, a critical component is the statistical relationship between habitat suitability and local population sizes. This study explores this relationship in two Enyalius lizard species from the Brazilian Atlantic Forest: the high-elevation E. iheringii and low-elevation E. catenatus and how this transformation affects spatiotemporal demographic inference. Most previous iDDC studies assumed a linear relationship, but this study hypothesises that the relationship may be nonlinear, especially for high-elevation species with broader environmental tolerances. We test two key hypotheses: (1) The habitat suitability to population size relationship is nonlinear for E. iheringii (high-elevation) and linear for E. catenatus (low-elevation); and (2) E. iheringii exhibits higher effective migration across populations than E. catenatus. Our findings provide clear support for hypothesis (2), but mixed support for hypothesis (1), with strong model support for a nonlinear transformation in the high-elevation E. iheringii and some (albeit weak) support for a nonlinear transformation also in E. catenatus. The iDDC models allow us to generate landscape-wide maps of predicted genetic diversity for both species, revealing that genetic diversity predictions for the high-elevation E. iheringii align with estimated patterns of historical range stability, whereas predictions for low-elevation E. catenatus are distinct from range-wide stability predictions. This research highlights the importance of accurately modelling the habitat suitability to population size relationship in iDDC studies, contributing to our understanding of species' demographic responses to environmental changes.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17593"},"PeriodicalIF":4.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Michael C. Whitlock-Recipient of the 2024 Molecular Ecology Prize. 迈克尔-惠特洛克(Michael C. Whitlock)-2024 年分子生态学奖获得者。
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-21 DOI: 10.1111/mec.17598
Sam Yeaman
{"title":"Michael C. Whitlock-Recipient of the 2024 Molecular Ecology Prize.","authors":"Sam Yeaman","doi":"10.1111/mec.17598","DOIUrl":"https://doi.org/10.1111/mec.17598","url":null,"abstract":"","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17598"},"PeriodicalIF":4.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Genomic Signature and Transcriptional Response of Metal Tolerance in Brown Trout Inhabiting Metal-Polluted Rivers. 栖息在受金属污染河流中的褐鳟耐受金属的基因组特征和转录反应
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-19 DOI: 10.1111/mec.17591
Josephine R Paris, R Andrew King, Joan Ferrer Obiol, Sophie Shaw, Anke Lange, Vincent Bourret, Patrick B Hamilton, Darren Rowe, Lauren V Laing, Audrey Farbos, Karen Moore, Mauricio A Urbina, Ronny van Aerle, Julian M Catchen, Rod W Wilson, Nicolas R Bury, Eduarda M Santos, Jamie R Stevens

Industrial pollution is a major driver of ecosystem degradation, but it can also act as a driver of contemporary evolution. As a result of intense mining activity during the Industrial Revolution, several rivers across the southwest of England are polluted with high concentrations of metals. Despite the documented negative impacts of ongoing metal pollution, brown trout (Salmo trutta L.) survive and thrive in many of these metal-impacted rivers. We used population genomics, transcriptomics, and metal burdens to investigate the genomic and transcriptomic signatures of potential metal tolerance. RADseq analysis of six populations (originating from three metal-impacted and three control rivers) revealed strong genetic substructuring between impacted and control populations. We identified selection signatures at 122 loci, including genes related to metal homeostasis and oxidative stress. Trout sampled from metal-impacted rivers exhibited significantly higher tissue concentrations of cadmium, copper, nickel and zinc, which remained elevated after 11 days in metal-free water. After depuration, we used RNAseq to quantify gene expression differences between metal-impacted and control trout, identifying 2042 differentially expressed genes (DEGs) in the gill, and 311 DEGs in the liver. Transcriptomic signatures in the gill were enriched for genes involved in ion transport processes, metal homeostasis, oxidative stress, hypoxia, and response to xenobiotics. Our findings reveal shared genomic and transcriptomic pathways involved in detoxification, oxidative stress responses and ion regulation. Overall, our results demonstrate the diverse effects of metal pollution in shaping both neutral and adaptive genetic variation, whilst also highlighting the potential role of constitutive gene expression in promoting metal tolerance.

工业污染是生态系统退化的主要驱动力,但它也可以成为当代进化的驱动力。由于工业革命期间采矿活动频繁,英格兰西南部的几条河流都受到了高浓度金属污染。尽管有文献记载持续的金属污染会带来负面影响,但褐鳟鱼(Salmo trutta L.)仍在这些受金属影响的河流中生存和繁衍。我们利用种群基因组学、转录组学和金属负荷来研究潜在金属耐受性的基因组和转录组特征。对六个种群(分别来自三条受金属影响的河流和三条对照河流)的 RADseq 分析显示,受影响种群和对照种群之间存在强烈的遗传亚结构。我们确定了 122 个基因位点的选择特征,包括与金属平衡和氧化应激相关的基因。在受金属影响的河流中采样的鳟鱼,其组织中的镉、铜、镍和锌浓度明显较高,在无金属水体中浸泡 11 天后,这些浓度仍保持在较高水平。净化后,我们使用 RNAseq 对受金属影响的鳟鱼和对照组鳟鱼之间的基因表达差异进行了量化,在鳃中发现了 2042 个差异表达基因(DEGs),在肝脏中发现了 311 个差异表达基因(DEGs)。鳃中的转录组特征富集于参与离子转运过程、金属稳态、氧化应激、缺氧和对异种生物反应的基因。我们的研究结果揭示了涉及解毒、氧化应激反应和离子调节的共同基因组和转录组通路。总之,我们的研究结果表明了金属污染在形成中性和适应性遗传变异方面的不同影响,同时也强调了组成型基因表达在促进金属耐受性方面的潜在作用。
{"title":"The Genomic Signature and Transcriptional Response of Metal Tolerance in Brown Trout Inhabiting Metal-Polluted Rivers.","authors":"Josephine R Paris, R Andrew King, Joan Ferrer Obiol, Sophie Shaw, Anke Lange, Vincent Bourret, Patrick B Hamilton, Darren Rowe, Lauren V Laing, Audrey Farbos, Karen Moore, Mauricio A Urbina, Ronny van Aerle, Julian M Catchen, Rod W Wilson, Nicolas R Bury, Eduarda M Santos, Jamie R Stevens","doi":"10.1111/mec.17591","DOIUrl":"10.1111/mec.17591","url":null,"abstract":"<p><p>Industrial pollution is a major driver of ecosystem degradation, but it can also act as a driver of contemporary evolution. As a result of intense mining activity during the Industrial Revolution, several rivers across the southwest of England are polluted with high concentrations of metals. Despite the documented negative impacts of ongoing metal pollution, brown trout (Salmo trutta L.) survive and thrive in many of these metal-impacted rivers. We used population genomics, transcriptomics, and metal burdens to investigate the genomic and transcriptomic signatures of potential metal tolerance. RADseq analysis of six populations (originating from three metal-impacted and three control rivers) revealed strong genetic substructuring between impacted and control populations. We identified selection signatures at 122 loci, including genes related to metal homeostasis and oxidative stress. Trout sampled from metal-impacted rivers exhibited significantly higher tissue concentrations of cadmium, copper, nickel and zinc, which remained elevated after 11 days in metal-free water. After depuration, we used RNAseq to quantify gene expression differences between metal-impacted and control trout, identifying 2042 differentially expressed genes (DEGs) in the gill, and 311 DEGs in the liver. Transcriptomic signatures in the gill were enriched for genes involved in ion transport processes, metal homeostasis, oxidative stress, hypoxia, and response to xenobiotics. Our findings reveal shared genomic and transcriptomic pathways involved in detoxification, oxidative stress responses and ion regulation. Overall, our results demonstrate the diverse effects of metal pollution in shaping both neutral and adaptive genetic variation, whilst also highlighting the potential role of constitutive gene expression in promoting metal tolerance.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17591"},"PeriodicalIF":4.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic Architecture Underlying the Striking Colour Variation in the Presence of Gene Flow for the Guinan Toad-Headed Lizard. 基因组结构是圭亚那蟾首蜥在基因流动情况下出现惊人颜色变异的基础。
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-15 DOI: 10.1111/mec.17594
Ying Chen, Song Tan, Qiwei Xu, Jinzhong Fu, Yin Qi, Xia Qiu, Weizhao Yang

How divergence occurs between closely related organisms in the absence of geographic barriers to gene flow stands as one of the long-standing questions in evolutionary biology. Previous studies suggested that the interplay between selection, gene flow and recombination strongly affected the process of divergence with gene flow. However, the extent to which these forces interact to drive divergence remains largely ambiguous. Guinan toad-headed lizards (Phrynocephalus guinanensis) in the Mugetan Desert exhibit striking colour differences from lizards outside the desert and provide an excellent model to address this question. Through extensive sampling and whole genome sequencing, we obtained genotypes for 191 samples from 14 populations inside and outside the desert. Despite the colour differences, continuous and asymmetric gene flow was detected across the desert border. More importantly, 273 highly diverged regions (HDRs) were identified between them, accounting only for 0.47% of the genome but widely distributed across 20 (out of the total 24) chromosomes. Strong signatures of selection were identified in HDRs, and local recombination rates were repressed. Furthermore, five HDRs exhibited significantly higher divergence, which contained key genes associated with crucial functions in animal coloration, including pteridine and melanocyte pigmentation. Genes related to retinal cells and steroid hormones were identified in other HDRs, which might have also contributed to the formation of colour variation in the presence of gene flow. This study provided novel insights into the understanding of the evolutionary mechanisms of genetic divergence in the presence of gene flow.

在基因流动没有地理障碍的情况下,近缘生物之间如何发生分化,是进化生物学中一个长期存在的问题。以往的研究表明,选择、基因流和重组之间的相互作用对基因流的分化过程有很大影响。然而,这些力量在多大程度上相互作用推动了物种的分化在很大程度上仍不明确。穆盖坦沙漠中的桂南蟾头蜥与沙漠外的蜥蜴在颜色上有显著差异,为解决这一问题提供了一个极好的模型。通过大量取样和全基因组测序,我们获得了来自沙漠内外 14 个种群 191 个样本的基因型。尽管存在肤色差异,但我们发现了跨越沙漠边界的持续且不对称的基因流动。更重要的是,在它们之间发现了 273 个高度分化区(HDR),仅占基因组的 0.47%,但广泛分布于 20 条染色体(共 24 条)上。在 HDRs 中发现了强烈的选择特征,局部重组率受到抑制。此外,有五个 HDRs 的分化程度明显更高,其中包含与动物着色的关键功能有关的关键基因,包括蝶啶和黑色素细胞色素沉着。在其他 HDR 中还发现了与视网膜细胞和类固醇激素有关的基因,这些基因也可能在基因流动的情况下对颜色变异的形成做出了贡献。这项研究为了解基因流动情况下基因分化的进化机制提供了新的见解。
{"title":"Genomic Architecture Underlying the Striking Colour Variation in the Presence of Gene Flow for the Guinan Toad-Headed Lizard.","authors":"Ying Chen, Song Tan, Qiwei Xu, Jinzhong Fu, Yin Qi, Xia Qiu, Weizhao Yang","doi":"10.1111/mec.17594","DOIUrl":"https://doi.org/10.1111/mec.17594","url":null,"abstract":"<p><p>How divergence occurs between closely related organisms in the absence of geographic barriers to gene flow stands as one of the long-standing questions in evolutionary biology. Previous studies suggested that the interplay between selection, gene flow and recombination strongly affected the process of divergence with gene flow. However, the extent to which these forces interact to drive divergence remains largely ambiguous. Guinan toad-headed lizards (Phrynocephalus guinanensis) in the Mugetan Desert exhibit striking colour differences from lizards outside the desert and provide an excellent model to address this question. Through extensive sampling and whole genome sequencing, we obtained genotypes for 191 samples from 14 populations inside and outside the desert. Despite the colour differences, continuous and asymmetric gene flow was detected across the desert border. More importantly, 273 highly diverged regions (HDRs) were identified between them, accounting only for 0.47% of the genome but widely distributed across 20 (out of the total 24) chromosomes. Strong signatures of selection were identified in HDRs, and local recombination rates were repressed. Furthermore, five HDRs exhibited significantly higher divergence, which contained key genes associated with crucial functions in animal coloration, including pteridine and melanocyte pigmentation. Genes related to retinal cells and steroid hormones were identified in other HDRs, which might have also contributed to the formation of colour variation in the presence of gene flow. This study provided novel insights into the understanding of the evolutionary mechanisms of genetic divergence in the presence of gene flow.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17594"},"PeriodicalIF":4.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Can Transcriptomics Elucidate the Role of Regulation in Invasion Success? 转录组学能否阐明调控在入侵成功中的作用?
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-15 DOI: 10.1111/mec.17583
Amy L Vaughan, Manpreet K Dhami

When a species invades a novel environment, it must bridge the environment-phenotype mismatch in its new range to persist. Contemporary invasion biology research has focused on the role that trait variation and adaptation, and their underlying genomic factors, play in a species' adaptive potential, and thus facilitating invasion. Empirical studies have provided valuable insights into phenotypes that persist and arise in novel environments, coupled with 'omics tools that further the understanding of the contributions of genomic architecture in species establishment. Particularly, the use of transcriptomics to explore the role of plasticity in the initial stages of an invasion is growing. Here, we assess the role of various mechanisms relating to regulation and functional adaptation (often measured via the transcriptome) that support trait-specific plasticity in invasive species, allowing phenotypic variability without directly altering genomic diversity. First, we present a comprehensive review of the studies utilising transcriptomics in invasion biology. Second, we collate the evidence for and against the role of a range of regulatory processes in contributing to invasive species plasticity. Finally, we pose open questions in invasion biology where the use of transcriptome data may be valuable, as well as discuss the methodological limitations.

当一个物种入侵一个新的环境时,它必须弥合其新分布区的环境-表型不匹配问题,才能持续生存下去。当代入侵生物学研究的重点是性状变异和适应性及其潜在的基因组因素在物种适应潜力中的作用,从而促进入侵。经验性研究为了解在新环境中持续存在和出现的表型提供了宝贵的见解,而'omics'工具则进一步加深了人们对基因组结构在物种建立过程中所作贡献的理解。特别是,利用转录组学探索可塑性在入侵初始阶段所起作用的研究越来越多。在这里,我们将评估与调控和功能适应有关的各种机制(通常通过转录组进行测量)的作用,这些机制支持入侵物种的特异性可塑性,从而在不直接改变基因组多样性的情况下实现表型变异。首先,我们全面回顾了入侵生物学中利用转录组学进行的研究。其次,我们整理了支持和反对一系列调控过程在促进入侵物种可塑性方面作用的证据。最后,我们提出了入侵生物学中利用转录组数据可能有价值的开放性问题,并讨论了方法上的局限性。
{"title":"Can Transcriptomics Elucidate the Role of Regulation in Invasion Success?","authors":"Amy L Vaughan, Manpreet K Dhami","doi":"10.1111/mec.17583","DOIUrl":"10.1111/mec.17583","url":null,"abstract":"<p><p>When a species invades a novel environment, it must bridge the environment-phenotype mismatch in its new range to persist. Contemporary invasion biology research has focused on the role that trait variation and adaptation, and their underlying genomic factors, play in a species' adaptive potential, and thus facilitating invasion. Empirical studies have provided valuable insights into phenotypes that persist and arise in novel environments, coupled with 'omics tools that further the understanding of the contributions of genomic architecture in species establishment. Particularly, the use of transcriptomics to explore the role of plasticity in the initial stages of an invasion is growing. Here, we assess the role of various mechanisms relating to regulation and functional adaptation (often measured via the transcriptome) that support trait-specific plasticity in invasive species, allowing phenotypic variability without directly altering genomic diversity. First, we present a comprehensive review of the studies utilising transcriptomics in invasion biology. Second, we collate the evidence for and against the role of a range of regulatory processes in contributing to invasive species plasticity. Finally, we pose open questions in invasion biology where the use of transcriptome data may be valuable, as well as discuss the methodological limitations.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17583"},"PeriodicalIF":4.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Secondary contact erodes Pleistocene diversification in a wide-ranging freshwater mussel (Quadrula). 二次接触侵蚀了范围广泛的淡水贻贝(Quadrula)的更新世多样化。
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-14 DOI: 10.1111/mec.17572
Sean M Keogh, Nathan A Johnson, Chase H Smith, Bernard E Sietman, Jeffrey T Garner, Charles R Randklev, Andrew M Simons

The isolated river drainages of eastern North America serve as a natural laboratory to investigate the roles of allopatry and secondary contact in the evolutionary trajectories of recently diverged lineages. Drainage divides facilitate allopatric speciation, but due to their sensitivity to climatic and geomorphological changes, neighboring rivers frequently coalesce, creating recurrent opportunities of isolation and contact throughout the history of aquatic lineages. The freshwater mussel Quadrula quadrula is widely distributed across isolated rivers of eastern North America and possesses high phenotypic and molecular variation across its range. We integrate sequence data from three genomes, including female- and male-inherited mitochondrial markers and thousands of nuclear encoded SNPs with morphology and geography to illuminate the group's divergence history. Across contemporary isolated rivers, we found continuums of molecular and morphological variation, following a pattern of isolation by distance. In contact zones, hybridization was frequent with no apparent fitness consequences, as advanced hybrids were common. Accordingly, we recognize Q. quadrula as a single cohesive species with subspecific variation (Q. quadrula rumphiana). Demographic modeling and divergence dating supported a divergence history characterized by allopatric vicariance followed by secondary contact, likely driven by river rearrangements and Pleistocene glacial cycles. Despite clinal range-wide variation and hybridization in contact zones, the process-based species delimitation tool delimitR, which considers demographic scenarios like secondary contact, supported the delimitation of the maximum number of species tested. As such, when interpreting species delimitation results, we suggest careful consideration of spatial sampling and subsequent geographic patterns of biological variation, particularly for wide-ranging taxa.

北美东部与世隔绝的河流流域是一个天然的实验室,可用于研究异源繁殖和二次接触在新近分化的物种进化轨迹中的作用。排水沟有利于同域物种的分化,但由于其对气候和地貌变化的敏感性,相邻的河流经常聚合在一起,在整个水生物种的历史中反复出现隔离和接触的机会。淡水贻贝 Quadrula quadrula 广泛分布于北美东部的孤立河流中,在其分布范围内具有很高的表型和分子变异。我们整合了来自三个基因组的序列数据,包括雌性和雄性遗传的线粒体标记和数千个核编码 SNPs,并结合形态学和地理学来阐明该群体的分化历史。在当代孤立的河流中,我们发现了分子和形态变异的连续性,遵循着因距离而隔离的模式。在接触区,杂交很频繁,但没有明显的适应性后果,因为高级杂交种很常见。因此,我们认为 Q. quadrula 是一个具有亚种变异的单一内聚物种(Q. quadrula rumphiana)。人口模型和分化年代测定支持了一个分化历史,其特点是同域沧桑,然后是二次接触,很可能是由河流重新排列和更新世冰川周期驱动的。尽管在接触区出现了全范围的支系变异和杂交,但基于过程的物种划分工具 delimitR(考虑了二次接触等人口统计情景)支持划分最多的测试物种。因此,在解释物种划分结果时,我们建议仔细考虑空间取样和随后的生物变异地理模式,特别是对于广域类群。
{"title":"Secondary contact erodes Pleistocene diversification in a wide-ranging freshwater mussel (Quadrula).","authors":"Sean M Keogh, Nathan A Johnson, Chase H Smith, Bernard E Sietman, Jeffrey T Garner, Charles R Randklev, Andrew M Simons","doi":"10.1111/mec.17572","DOIUrl":"10.1111/mec.17572","url":null,"abstract":"<p><p>The isolated river drainages of eastern North America serve as a natural laboratory to investigate the roles of allopatry and secondary contact in the evolutionary trajectories of recently diverged lineages. Drainage divides facilitate allopatric speciation, but due to their sensitivity to climatic and geomorphological changes, neighboring rivers frequently coalesce, creating recurrent opportunities of isolation and contact throughout the history of aquatic lineages. The freshwater mussel Quadrula quadrula is widely distributed across isolated rivers of eastern North America and possesses high phenotypic and molecular variation across its range. We integrate sequence data from three genomes, including female- and male-inherited mitochondrial markers and thousands of nuclear encoded SNPs with morphology and geography to illuminate the group's divergence history. Across contemporary isolated rivers, we found continuums of molecular and morphological variation, following a pattern of isolation by distance. In contact zones, hybridization was frequent with no apparent fitness consequences, as advanced hybrids were common. Accordingly, we recognize Q. quadrula as a single cohesive species with subspecific variation (Q. quadrula rumphiana). Demographic modeling and divergence dating supported a divergence history characterized by allopatric vicariance followed by secondary contact, likely driven by river rearrangements and Pleistocene glacial cycles. Despite clinal range-wide variation and hybridization in contact zones, the process-based species delimitation tool delimitR, which considers demographic scenarios like secondary contact, supported the delimitation of the maximum number of species tested. As such, when interpreting species delimitation results, we suggest careful consideration of spatial sampling and subsequent geographic patterns of biological variation, particularly for wide-ranging taxa.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17572"},"PeriodicalIF":4.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Infection by the Lungworm Rhabdias pseudosphaerocephala Affects the Expression of Immune-Related microRNAs by Its Co-Evolved Host, the Cane Toad Rhinella marina. 肺线虫 Rhabdias pseudosphaerocephala 的感染会影响其共同进化宿主蔗蟾 Rhinella marina 与免疫相关的 microRNAs 的表达。
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-14 DOI: 10.1111/mec.17587
Tsering C L Chan, Boris Yagound, Gregory P Brown, Harrison J F Eyck, Richard Shine, Lee A Rollins

Parasites may suppress the immune function of infected hosts using microRNAs (miRNAs) to prevent protein production. Nonetheless, little is known about the diversity of miRNAs and their mode(s) of action. In this study, we investigated the effects of infection by a parasitic lungworm (Rhabdias pseudosphaerocephala) on miRNA and mRNA expression of its host, the invasive cane toad (Rhinella marina). To investigate the cane toad's innate and adaptive immune response to this parasite, we compared miRNA and mRNA expression in naïve toads that had never been infected by lungworms to toads that were infected with lungworms for the first time in their lives, and toads that were infected the second time in their lives (i.e., had two consecutive infections). In total, we identified 101 known miRNAs and 86 potential novel miRNAs. Compared to uninfected and single-infection toads, multiple-infection animals drastically downregulated three miRNAs. These miRNAs were associated with gene pathways related to the immune response, potentially reflecting the immunosuppression of cane toads by their parasites. Infected hosts did not respond with substantially differential mRNA transcription; only one gene was differentially expressed between control and single-infection hosts. Our study suggests that miRNA may play an important role in mediating host-parasite interactions in a system in which an ongoing range expansion by the host has generated substantial divergence in host-parasite interactions.

寄生虫可能会利用微RNA(miRNA)抑制受感染宿主的免疫功能,从而阻止蛋白质的产生。然而,人们对 miRNAs 的多样性及其作用模式知之甚少。在这项研究中,我们调查了寄生肺线虫(Rhabdias pseudosphaerocephala)感染对其宿主--入侵蔗蟾(Rhinella marina)--的miRNA和mRNA表达的影响。为了研究蔗蟾对这种寄生虫的先天性和适应性免疫反应,我们比较了从未感染过肺蠕虫的天真蟾蜍、生平第一次感染肺蠕虫的蟾蜍和生平第二次感染肺蠕虫的蟾蜍(即连续两次感染)的 miRNA 和 mRNA 表达。我们总共鉴定了 101 个已知的 miRNA 和 86 个潜在的新型 miRNA。与未感染和单次感染的蟾蜍相比,多次感染的动物大幅下调了三种 miRNA。这些miRNA与免疫反应相关的基因通路有关,可能反映了寄生虫对蔗蟾的免疫抑制。受感染宿主的 mRNA 转录没有显著差异;只有一个基因在对照宿主和单一感染宿主之间有差异表达。我们的研究表明,miRNA 在宿主与寄生虫之间的相互作用中可能起着重要的介导作用,在这个系统中,宿主的活动范围不断扩大,导致宿主与寄生虫之间的相互作用出现了很大的差异。
{"title":"Infection by the Lungworm Rhabdias pseudosphaerocephala Affects the Expression of Immune-Related microRNAs by Its Co-Evolved Host, the Cane Toad Rhinella marina.","authors":"Tsering C L Chan, Boris Yagound, Gregory P Brown, Harrison J F Eyck, Richard Shine, Lee A Rollins","doi":"10.1111/mec.17587","DOIUrl":"10.1111/mec.17587","url":null,"abstract":"<p><p>Parasites may suppress the immune function of infected hosts using microRNAs (miRNAs) to prevent protein production. Nonetheless, little is known about the diversity of miRNAs and their mode(s) of action. In this study, we investigated the effects of infection by a parasitic lungworm (Rhabdias pseudosphaerocephala) on miRNA and mRNA expression of its host, the invasive cane toad (Rhinella marina). To investigate the cane toad's innate and adaptive immune response to this parasite, we compared miRNA and mRNA expression in naïve toads that had never been infected by lungworms to toads that were infected with lungworms for the first time in their lives, and toads that were infected the second time in their lives (i.e., had two consecutive infections). In total, we identified 101 known miRNAs and 86 potential novel miRNAs. Compared to uninfected and single-infection toads, multiple-infection animals drastically downregulated three miRNAs. These miRNAs were associated with gene pathways related to the immune response, potentially reflecting the immunosuppression of cane toads by their parasites. Infected hosts did not respond with substantially differential mRNA transcription; only one gene was differentially expressed between control and single-infection hosts. Our study suggests that miRNA may play an important role in mediating host-parasite interactions in a system in which an ongoing range expansion by the host has generated substantial divergence in host-parasite interactions.</p>","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17587"},"PeriodicalIF":4.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Natural Hybrid Zone of Swordtails Reveals Molecular Insights Into the Adaptive Genomic Basis of Thermal Tolerance. 剑尾鱼自然杂交区揭示了耐热性适应性基因组基础的分子观点。
IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-14 DOI: 10.1111/mec.17584
Carina M Lai, Brenna C M Stanford, Sean M Rogers
{"title":"A Natural Hybrid Zone of Swordtails Reveals Molecular Insights Into the Adaptive Genomic Basis of Thermal Tolerance.","authors":"Carina M Lai, Brenna C M Stanford, Sean M Rogers","doi":"10.1111/mec.17584","DOIUrl":"https://doi.org/10.1111/mec.17584","url":null,"abstract":"","PeriodicalId":210,"journal":{"name":"Molecular Ecology","volume":" ","pages":"e17584"},"PeriodicalIF":4.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Molecular Ecology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1