Monoclonal antibody based colloidal gold immunochromatographic assay for the visual and rapid screening of profenofos

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Environmental Pollution Pub Date : 2024-08-29 DOI:10.1016/j.envpol.2024.124848

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Abstract

Profenofos (PFF) is a commonly used organophosphorus insecticide that requires strict monitoring due to its potential environmental, ecological, and human health risks originating from residues in soil and water systems, as well as accumulation in crops. In this study, a novel monoclonal antibody (mAb) specific to PFF was prepared for the first time and the recognition mechanism was investigated through molecular simulation. Subsequently, a mAb-based colloidal gold immunochromatographic assay (GICA) was developed for the rapid screening of PFF in fruit and vegetable samples. The mAb exhibited an IC₅₀ value of 12.9 ng/mL, and limit of detection (LOD) of 4.6 ng/mL, respectively in indirect competitive immunosorbent enzyme-linked immunosorbent assay (ic-ELISA). After optimization, the developed GICA exhibited a visual limit of detection (vLOD) of 20 ng/mL and a quantitative of detection (qLOD) of 5.2 ng/mL, with a linear range from 10.0 to 83.8 ng/mL. Good correlation was observed between the results of GICA and standard Gas Chromatography-Tandem Mass Spectrometry (GC-MS/MS) in matrix and recovery test. The developed GICA can be used for rapid sample detection within 15 min, which is an excellent tool for screening PFF in foods and environmental samples.

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基于单克隆抗体的胶体金免疫层析测定法，用于直观、快速筛查丙溴磷。

丙溴磷（PFF）是一种常用的有机磷杀虫剂，由于其在土壤和水系统中的残留以及在农作物中的蓄积可能对环境、生态和人类健康造成危害，因此需要对其进行严格监控。本研究首次制备了针对 PFF 的新型单克隆抗体（mAb），并通过分子模拟研究了其识别机制。随后，研究人员开发了一种基于 mAb 的胶体金免疫层析（GICA）方法，用于快速筛查果蔬样品中的 PFF。在间接竞争性免疫吸附酶联免疫吸附试验（ic-ELISA）中，该 mAb 的 IC50 值为 12.9 ng/mL，检出限（LOD）为 4.6 ng/mL。经过优化后，所开发的 GICA 可视检测限（vLOD）为 20 纳克/毫升，定量检测限（qLOD）为 5.2 纳克/毫升，线性范围为 10.0 至 83.8 纳克/毫升。在基质和回收率测试中，GICA 与标准气相色谱-串联质谱法（GC-MS/MS）的结果具有良好的相关性。所开发的 GICA 可在 15 分钟内对样品进行快速检测，是筛查食品和环境样品中 PFF 的绝佳工具。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.