A morpho-viscoelasticity theory for growth in proliferating aggregates

Abstract

Despite significant research efforts in the continuum modeling of biological growth, certain aspects have been overlooked. For instance, numerous investigations have examined the influence of morphogenetic cell behaviors, like division and intercalation, on the mechanical response of passive (non-growing) tissues. Yet, their impact on active growth dynamics remains inadequately explored. A key reason for this inadequacy stems from challenges in the continuum treatment of cell-level processes. While some coarse-grained models have been proposed to address these shortcomings, a focus on cell division and cell expansion has been missing, rendering them unusable when it comes to modeling growth. Moreover, existing studies are limited to two-dimensional tissues and are yet to be formally extended to three-dimensional multicellular systems. To address these limitations, we here present a generalized multiscale model for three-dimensional aggregates that accounts for complex morphogenetic movements that include division, expansion, and intercalation. The proposed continuum theory thus allows for a comprehensive exploration into the growth and dissipation mechanics of proliferating aggregates, such as spheroids and organoids.