Solution NMR backbone assignment of the N-terminal tandem Zα1-Zα2 domains of Z-DNA binding protein 1

IF 0.8 4区 生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2024-08-31 DOI:10.1007/s12104-024-10195-1
Lily G. Beck, Jeffrey B. Krall, Parker J. Nichols, Quentin Vicens, Morkos A. Henen, Beat Vögeli
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Abstract

The detection of nucleic acids that are present in atypical conformations is a crucial trigger of the innate immune response. Human Z-DNA binding protein 1 (ZBP1) is a pattern recognition receptor that harbors two Zα domains that recognize Z-DNA and Z-RNA. ZBP1 detects this alternate nucleic acid conformation as foreign, and upon stabilization of these substrates, it triggers activation of an immune response. Here, we present the backbone chemical shift assignment of a construct encompassing the Zα1 and Zα2 domains as well as the interconnecting linker of ZBP1. These assignments can be directly transferred to the isolated Zα1 and Zα2 domains, thereby demonstrating that these domains maintain virtually identical structures in the tandem context.

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Z-DNA 结合蛋白 1 N 端串联 Zα1-Zα2 结构域的溶液核磁共振骨架分配。
检测以非典型构象存在的核酸是触发先天性免疫反应的一个关键因素。人类 Z-DNA 结合蛋白 1(ZBP1)是一种模式识别受体,含有两个 Zα 结构域,可识别 Z-DNA 和 Z-RNA。ZBP1 将这种交替的核酸构象检测为外来物,当这些底物稳定时,ZBP1 就会触发免疫反应。在这里,我们介绍了一种包含 Zα1 和 Zα2 结构域以及 ZBP1 的互连连接体的构建物的骨架化学位移分配。这些分配可以直接转移到孤立的 Zα1 和 Zα2 结构域,从而证明这些结构域在串联情况下保持着几乎完全相同的结构。
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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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