High-resolution X-ray computed tomography for identifying herbal medicines was as effective as microscopic examination

IF 2.5 4区 医学 Q3 CHEMISTRY, MEDICINAL Journal of Natural Medicines Pub Date : 2024-08-29 DOI:10.1007/s11418-024-01840-z
Hiroko Tokumoto, Nahoko Uchiyama, Michiho Ito
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Abstract

Microscopic examination is one of the important identification methods for crude drug test described in the 18th Japanese Pharmacopoeia. This method is useful for identification because it can be used for small amounts of samples regardless of their storage conditions; however, this method requires a lot of technical skill in sectioning intricate and/or small samples and is time-consuming. High-resolution X-ray computed tomography (HRXCT) is a novel method for observing the internal morphology of materials. Previously, we used HRXCT to visualize the internal morphology of the Ephedra Herb, obtaining observations that closely match those obtained via microscopic examination. HRXCT employs a low-energy X-ray source and the permeation distance of the X-rays is very short. Therefore, HRXCT can be used for elucidating the morphology of small herbal medicines. In this study, Artemisia Capillaris Flower (capitulum with a diameter of approximately 2 mm) and Plantago Seed (seeds with a length of approximately 2 mm) were examined. The results showed that HRXCT examination was sufficient to illustrate the internal independent organs of Artemisia Capillaris Flower and that their inflorescences remained intact. When observing Plantago Seed, the internal morphology of more than one seed can be depicted simultaneously. Therefore, observation using HRXCT was easy, simple, and effective to illustrate the internal morphology of herbal medicines, which is typically time-consuming and requires advanced microscopy skills.

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高分辨率 X 射线计算机断层扫描技术在鉴别草药方面与显微镜检查同样有效。
显微镜检查是《第 18 版日本药典》中描述的粗制药物测试的重要鉴定方法之一。这种方法适用于少量样品,不受其储存条件的限制,因此对鉴定非常有用;但是,这种方法在对复杂和/或少量样品进行切片时需要大量的技术技能,而且耗时较长。高分辨率 X 射线计算机断层扫描(HRXCT)是一种观察材料内部形态的新方法。此前,我们使用 HRXCT 观察了麻黄草的内部形态,观察结果与显微镜检查结果非常接近。HRXCT 采用低能量 X 射线源,X 射线的穿透距离非常短。因此,HRXCT 可用于阐明小药材的形态。本研究对茵陈蒿花(蒴果直径约 2 毫米)和车前子种子(种子长度约 2 毫米)进行了检测。结果表明,HRXCT 检查足以说明茵陈蒿花的内部独立器官及其花序保持完整。在观察车前子种子时,可以同时描绘多个种子的内部形态。因此,使用 HRXCT 观察说明中药材的内部形态既方便又简单有效,而这通常是费时且需要高级显微技术的。
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来源期刊
CiteScore
6.90
自引率
3.00%
发文量
79
审稿时长
1.7 months
期刊介绍: The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.
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