Arsenic trioxide induces innate immune response and inflammatory response in chicken liver via cGAS-STING/NF-κB pathway

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology C-toxicology & Pharmacology Pub Date : 2024-08-30 DOI:10.1016/j.cbpc.2024.110017
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Abstract

Arsenic is a toxic metal-like element widely used in the pesticide, preservative and semiconductor industries. However, accumulation of arsenic through the food chain can cause serious damage to animal and human health. However, the toxic mechanism of arsenic-induced hepatotoxicity in chickens is not clear, and the present study aimed to investigate the potential role of cGAS-STING and NF-κB pathways on inflammatory injury in chicken liver. In this study, 75 white-feathered broilers were divided into a control group, a low-dose arsenic group (4 mg/kg) and a high-dose arsenic group (8 mg/kg) to investigate the toxic effects of arsenic on chicken liver. In this study, we found that pathological changes such as inflammatory cell infiltration and vesicular degeneration occurred in the liver when exposed to ATO. Crucially, exposure to ATO triggered the cGAS-STING pathway and markedly raised the levels of mRNA and protein expression of cGAS, STING, TBK1, and IRF7. The type I interferon response was also triggered. Simultaneously, STING induced the activation of the conventional NF-κB signaling pathway and stimulated the expression of genes associated with inflammation, such as IL-6, TNF-α and IL-1β. In summary, the induction of inflammatory responses via cGAS-STING and NF-κB signaling pathways under high ATO exposure provides new ideas for further studies on the toxicological mechanisms of arsenic.

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三氧化二砷通过cGAS-STING/NF-κB途径诱导鸡肝的先天免疫反应和炎症反应
砷是一种有毒的类金属元素,广泛用于杀虫剂、防腐剂和半导体工业。然而,砷在食物链中的累积会对动物和人类健康造成严重损害。本研究旨在探讨 cGAS-STING 和 NF-κB 通路对鸡肝炎症损伤的潜在作用。本研究将 75 只白羽肉鸡分为对照组、低剂量砷组(4 毫克/千克)和高剂量砷组(8 毫克/千克),以探讨砷对鸡肝的毒性作用。在这项研究中,我们发现当暴露于 ATO 时,肝脏会出现炎性细胞浸润和水泡变性等病理变化。最关键的是,暴露于 ATO 会触发 cGAS-STING 通路,并显著提高 cGAS、STING、TBK1 和 IRF7 的 mRNA 和蛋白表达水平。I 型干扰素反应也被触发。与此同时,STING 还诱导激活了传统的 NF-κB 信号通路,并刺激了与炎症相关的基因(如 IL-6、TNF-α 和 IL-1β)的表达。总之,高浓度 ATO 暴露下通过 cGAS-STING 和 NF-κB 信号通路诱导炎症反应为进一步研究砷的毒理机制提供了新思路。
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来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
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