Molecular detection and genotyping of HBV from HBsAg positive patients in a tertiary hospital in Nigeria.

Q2 Health Professions Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 DOI:10.1080/15321819.2024.2397377

Adedayo O Faneye, Aisha Mustafa, Babatunde O Motayo, Adewale V Opayele, Kolawole O Akande

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Abstract

Background: Nigeria remains one of the countries with a high hepatitis B virus (HBV) burden in Africa. Reports have indicated the presence of mixed HBV genotypes in Nigeria; however, there is still paucity of data regarding mixed genotype infections particularly in the Southern part of the country.

Objective: Our aim is to determine the HBV genotype distribution among HBsAg-positive gastroenterology patients at the University College Hospital Ibadan, Nigeria.

Method: Serum samples were screened for HBsAg by ELISA, and positive samples were genotyped by semi-nested multiplex PCR for HBV genotypes A, B, C, D, E and F.

Results: Data generated were analyzed in R-studio. A total of 81/90 (90%) of HBsAg-positive samples were successfully genotyped, and genotype A was most prevalent with 15.7%, while genotypes B and E were the least with 1.2% each. Genotypes A/C infection was the highest among mixed infections with 40% prevalence, while genotypes A/D were the least prevalent mixed infection with 4.8%.

Conclusion: We advocate for a comprehensive genotype analysis in larger cohorts across Nigeria, to give a more comprehensive understanding of the distribution and prevalence of different HBV genotypes population wide.

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尼日利亚一家三甲医院对 HBsAg 阳性患者的 HBV 进行分子检测和基因分型。

背景：尼日利亚仍然是非洲乙型肝炎病毒（HBV）负担较重的国家之一。有报告显示，尼日利亚存在混合型 HBV 基因型；然而，有关混合型基因型感染的数据仍然很少，尤其是在尼日利亚南部地区：我们的目的是确定尼日利亚伊巴丹大学学院医院 HBsAg 阳性肠胃病患者的 HBV 基因型分布情况：用 ELISA 对血清样本进行 HBsAg 检测，并用半嵌合多重 PCR 对阳性样本进行 HBV 基因型 A、B、C、D、E 和 F 的基因分型：用 R-studio 对生成的数据进行分析。共有 81/90 份（90%）HBsAg 阳性样本成功进行了基因分型，其中基因型 A 的感染率最高，为 15.7%，而基因型 B 和 E 的感染率最低，分别为 1.2%。在混合感染中，基因型 A/C 感染率最高，达 40%，而基因型 A/D 混合感染率最低，仅为 4.8%：我们主张在尼日利亚更大规模的队列中进行全面的基因型分析，以便更全面地了解不同 HBV 基因型在整个人群中的分布和流行情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of immunoassay & immunochemistry 医学-免疫学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.