Metagenomic evaluation of bacteria in drinking water using full-length 16S rRNA amplicons.

IF 2.5 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Journal of water and health Pub Date : 2024-08-01 Epub Date: 2024-07-30 DOI:10.2166/wh.2024.090
William Taylor, Megan Louise Devane, Kathryn Russell, Susan Lin, Colin Roxburgh, Judy Williamson, Brent John Gilpin
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Abstract

Escherichia coli and total coliforms are important tools for identifying potential faecal contamination in drinking water. However, metagenomics offers a powerful approach for delving deeper into a bacterial community when E. coli or total coliforms are detected. Metagenomics can identify microbes native to water systems, track community changes and potential pathogens introduced by contamination events, and evaluate the effectiveness of treatment processes. Here, we demonstrate how the dual application of traditional monitoring practices and metagenomics can improve monitoring and surveillance for water resource management. The robustness of long-read metagenomics across replicates is demonstrated by the effect and interaction between manganese filters and bacterial communities, as well as the impact of chlorination after coliform detection. These examples reveal how metagenomics can identify the complex bacterial communities in the distribution system and the source waters used to supply drinking water treatment plants (DWTPs). The knowledge gained increases confidence in identified causes and mitigations of potential contamination events. By exploring bacterial communities, we can gain additional insights into the impact of faecal contamination events and treatment processes. This insight enables more precise remediation actions and enhances confidence in communicating health risks to drinking water operators and the public.

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利用全长 16S rRNA 扩增子对饮用水中的细菌进行元基因组学评估。
大肠杆菌和总大肠菌群是识别饮用水中潜在粪便污染的重要工具。然而,当检测到大肠杆菌或总大肠菌群时,元基因组学提供了一种深入研究细菌群落的强大方法。元基因组学可以识别水系统中的原生微生物,跟踪污染事件引起的群落变化和潜在病原体,并评估处理过程的有效性。在这里,我们展示了传统监测方法和元基因组学的双重应用如何改善水资源管理的监测和监控。通过锰过滤器与细菌群落之间的影响和相互作用,以及大肠菌群检测后加氯的影响,证明了长读数元基因组学在不同重复中的稳健性。这些例子揭示了元基因组学如何识别配水系统中复杂的细菌群落以及饮用水处理厂(DWTP)的原水。所获得的知识增强了我们对潜在污染事件的原因和缓解措施的信心。通过探索细菌群落,我们可以深入了解粪便污染事件和处理过程的影响。这种洞察力有助于采取更精确的补救措施,并增强向饮用水运营商和公众传达健康风险的信心。
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来源期刊
Journal of water and health
Journal of water and health 环境科学-环境科学
CiteScore
3.60
自引率
8.70%
发文量
110
审稿时长
18-36 weeks
期刊介绍: Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.
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