The genetic variants of miR-1206 and miR-125b in breast cancer patients: in vitro and in silico analysis.

IF 1.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-08-30 DOI:10.1080/15257770.2024.2398548

Hamideh Amini Mostafaabadi, Reza Mohammadzadeh, Somayeh Reiisi

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Abstract

Background: This study aimed to investigate the association of rs12976445 polymorphism in the promoter region of miR-125a and rs2114358 in the precursor region of miR-1206 to breast cancer susceptibility.

Method: A total of 230 participants (110 breast cancer and 120 controls) enrolled in this study and extracted genomic DNA. The genotypes were determined by the Tetra-ARMS method. The allele and genotype frequencies were determined.

Results: Allele variation in the rs12976445 (miR-125a) sequence increased the risk of breast cancer; a significant relationship was observed between breast cancer and allele change in individuals with the C allele (p = 0.01). However, allele variation in the rs2114358 (miR-1206) decreased the risk of breast cancer in individuals with allele A (p = 0.01). In silico study showed that allele change was associated with a reduction in structural stability.

Conclusion: Therefore, the rs12976445 variant can be considered a risk factor for breast cancer, and the rs2114358 variant is a protective factor against it.

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乳腺癌患者体内 miR-1206 和 miR-125b 的基因变异：体外和硅学分析。

研究背景本研究旨在探讨miR-125a启动子区的rs12976445多态性和miR-1206前体区的rs2114358多态性与乳腺癌易感性的关系：共有 230 名参与者（110 名乳腺癌患者和 120 名对照组患者）参加了这项研究，并提取了基因组 DNA。基因型用 Tetra-ARMS 方法测定。测定等位基因和基因型频率：rs12976445（miR-125a）序列的等位基因变异增加了乳腺癌的患病风险；在具有 C 等位基因的个体中，乳腺癌与等位基因变化之间存在显著关系（p = 0.01）。然而，rs2114358（miR-1206）的等位基因变异会降低等位基因为 A 的个体罹患乳腺癌的风险（p = 0.01）。硅学研究表明，等位基因的变化与结构稳定性的降低有关：因此，rs12976445 变异可被视为乳腺癌的风险因素，而 rs2114358 变异则是乳腺癌的保护因素。

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来源期刊

Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

6 months

期刊介绍： Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.