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Pharmacological interventions that activate mitochondrial biogenesis stimulate nucleotide generation in isoproterenol-stressed rat cardiomyocytes.
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-02 DOI: 10.1080/15257770.2025.2453105
Alicja Braczko, Klaudia Stawarska, Ada Kawecka, Iga Walczak, Ewa M Slomińska, Barbara Kutryb-Zając, Ryszard T Smoleński

Mitochondrial dysfunction in failing hearts has been described as a driving force for energy deprivation and cardiomyocyte energy supply-demand imbalance. Isoproterenol (ISO), the β1/β2-adrenergic receptor agonist that leads to myocardial stress and mitochondrial damage, is extensively used for in vitro and in vivo studies to test the efficacy of therapeutic strategies in heart failure (HF). This study evaluated the cell morphology, nucleotide concentrations, and mitochondrial function of ISO-treated cardiomyocytes stimulated with the activators of mitochondrial biogenesis and nucleotide precursors. H9c2 rat cardiomyocyte line cells were treated with ISO in the presence of mitochondrial biogenesis stimuli quercetin (Que), rosiglitazone (Ros), S-Nitroso-N-acetyl-DL-penicillamin (SNAP), and NAD+ precursor, nicotinamide riboside (NR). The intracellular concentrations of nucleotides were analyzed using high-performance liquid chromato-graphy, and the Seahorse metabolic flux analyzer determined the mitochondrial function. ISO decreased intracellular ATP concentration in H9c2 cells as compared to control. The treatment with SNAP increased ATP concentration compared to ISO-only treated cells, while Que, Ros, and NR had no effect. NR treatment led to the elevation of intracellular NAD+ concentration, while the treatment with SNAP, Ros, and NR stimulated the mitochondrial respiration in ISO-pretreated H9c2 cells. In conclusion, mitochondrial biogenesis activators consistently improved cardiomyocyte mitochondrial function, but only selected molecules helped to improve ATP or NAD+ concentrations. This information may help to optimize treatment to ameliorate energy imbalance in failing cardiomyocytes.

{"title":"Pharmacological interventions that activate mitochondrial biogenesis stimulate nucleotide generation in isoproterenol-stressed rat cardiomyocytes.","authors":"Alicja Braczko, Klaudia Stawarska, Ada Kawecka, Iga Walczak, Ewa M Slomińska, Barbara Kutryb-Zając, Ryszard T Smoleński","doi":"10.1080/15257770.2025.2453105","DOIUrl":"https://doi.org/10.1080/15257770.2025.2453105","url":null,"abstract":"<p><p>Mitochondrial dysfunction in failing hearts has been described as a driving force for energy deprivation and cardiomyocyte energy supply-demand imbalance. Isoproterenol (ISO), the β1/β2-adrenergic receptor agonist that leads to myocardial stress and mitochondrial damage, is extensively used for <i>in vitro</i> and <i>in vivo</i> studies to test the efficacy of therapeutic strategies in heart failure (HF). This study evaluated the cell morphology, nucleotide concentrations, and mitochondrial function of ISO-treated cardiomyocytes stimulated with the activators of mitochondrial biogenesis and nucleotide precursors. H9c2 rat cardiomyocyte line cells were treated with ISO in the presence of mitochondrial biogenesis stimuli quercetin (Que), rosiglitazone (Ros), <i>S</i>-Nitroso-<i>N</i>-acetyl-DL-penicillamin (SNAP), and NAD<sup>+</sup> precursor, nicotinamide riboside (NR). The intracellular concentrations of nucleotides were analyzed using high-performance liquid chromato-graphy, and the Seahorse metabolic flux analyzer determined the mitochondrial function. ISO decreased intracellular ATP concentration in H9c2 cells as compared to control. The treatment with SNAP increased ATP concentration compared to ISO-only treated cells, while Que, Ros, and NR had no effect. NR treatment led to the elevation of intracellular NAD<sup>+</sup> concentration, while the treatment with SNAP, Ros, and NR stimulated the mitochondrial respiration in ISO-pretreated H9c2 cells. In conclusion, mitochondrial biogenesis activators consistently improved cardiomyocyte mitochondrial function, but only selected molecules helped to improve ATP or NAD<sup>+</sup> concentrations. This information may help to optimize treatment to ameliorate energy imbalance in failing cardiomyocytes.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-12"},"PeriodicalIF":1.1,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical diagnostic value and potential regulatory mechanisms of lncRNA NOP14-AS1 in chronic kidney disease.
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-25 DOI: 10.1080/15257770.2025.2456794
Hongfang Jiang, Huajuan Shen, Xiujun Xu, Yanna Liu, Yongze Dong, Jiaxiang Jiang

In the early stages, chronic kidney disease (CKD) can be asymptomatic, marking diagnosis difficult. This study aimed to investigate the diagnostic role and potential regulatory mechanisms of nucleolar protein 14 (NOP14) -antisense RNA 1 (AS1) in patients with CKD. Herein, 68 patients with CKD, 65 patients with CKD undergoing peridialysis, and 80 healthy adults were included. The real-time reverse transcription-quantitative polymerase chain reaction was performed to assess NOP14-AS1 levels, and its diagnostic value was evaluated using receiver operating characteristic curves. Additionally, cell proliferation and apoptosis were assessed by Cell Counting Kit-8 assay. and flow cytometry, respectively. Oxidative stress levels were determined using superoxide dismutase and malondialdehyde MDA kits, and the dual-luciferase reporter assay was performed to determine the relationship between NOP14-AS1 and microRNA-326 (miR-326) target binding. Lastly, the potential mechanism underlying miR-326 target gene regulation in CKD progression were explored utilizing Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Notably, patients with CKD exhibited decreasedNOP14-AS1 levels and upregulated miR-326 levels. NOP14-AS1 and miR-326 exhibited combined effects on cell proliferation, apoptosis, inflammatory factors, and oxidative stress levels. Furthermore, the target genes of miR-326 showed enrichment in CKD-associated rat sarcoma and phosphoinositide 3-kinase protein kinase B pathways. Altogether, the findings of this study show the potential of NOP14-AS1 as a diagnostic marker in CKD. Overall, NOP14-AS1 regulates the miR-326 expression, which, in turn, regulates various miR-326 target gene-associated signaling pathways, thereby affecting the occurrence and development of CKD.

{"title":"Clinical diagnostic value and potential regulatory mechanisms of lncRNA NOP14-AS1 in chronic kidney disease.","authors":"Hongfang Jiang, Huajuan Shen, Xiujun Xu, Yanna Liu, Yongze Dong, Jiaxiang Jiang","doi":"10.1080/15257770.2025.2456794","DOIUrl":"https://doi.org/10.1080/15257770.2025.2456794","url":null,"abstract":"<p><p>In the early stages, chronic kidney disease (CKD) can be asymptomatic, marking diagnosis difficult. This study aimed to investigate the diagnostic role and potential regulatory mechanisms of nucleolar protein 14 (NOP14) -antisense RNA 1 (AS1) in patients with CKD. Herein, 68 patients with CKD, 65 patients with CKD undergoing peridialysis, and 80 healthy adults were included. The real-time reverse transcription-quantitative polymerase chain reaction was performed to assess NOP14-AS1 levels, and its diagnostic value was evaluated using receiver operating characteristic curves. Additionally, cell proliferation and apoptosis were assessed by Cell Counting Kit-8 assay. and flow cytometry, respectively. Oxidative stress levels were determined using superoxide dismutase and malondialdehyde MDA kits, and the dual-luciferase reporter assay was performed to determine the relationship between NOP14-AS1 and microRNA-326 (miR-326) target binding. Lastly, the potential mechanism underlying miR-326 target gene regulation in CKD progression were explored utilizing Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Notably, patients with CKD exhibited decreasedNOP14-AS1 levels and upregulated miR-326 levels. NOP14-AS1 and miR-326 exhibited combined effects on cell proliferation, apoptosis, inflammatory factors, and oxidative stress levels. Furthermore, the target genes of miR-326 showed enrichment in CKD-associated rat sarcoma and phosphoinositide 3-kinase protein kinase B pathways. Altogether, the findings of this study show the potential of NOP14-AS1 as a diagnostic marker in CKD. Overall, NOP14-AS1 regulates the miR-326 expression, which, in turn, regulates various miR-326 target gene-associated signaling pathways, thereby affecting the occurrence and development of CKD.</p>","PeriodicalId":19343,"journal":{"name":"Nucleosides, Nucleotides & Nucleic Acids","volume":" ","pages":"1-18"},"PeriodicalIF":1.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sustainable synthesis of benzimidazole-based Schiff base using reusable CaAl2O4 nanophosphors catalyst: Insights into metal(II) complexes and DNA interactions. 使用可重复使用的CaAl2O4纳米磷光催化剂可持续合成苯并咪唑基希夫碱:金属(II)配合物和DNA相互作用的见解。
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-19 DOI: 10.1080/15257770.2025.2451375
M Manjunath, F H Sujata, A H Shridhara, B Vinay Kumar, K Prashantha, K Yogendra, N Madhusudhana

This article presents a new and facile method for the synthesis of Schiff base compounds with a benzimidazole group using a low-cost and reusable calcium aluminate nanophosphorus catalyst (CaAl2O4). This approach avoids harmful solvents and reactants, supporting a more environmentally friendly synthesis process. The catalyst maintained its activity and heterogeneity over four cycles with minimal loss of efficiency. The synthesis process was straightforward and eliminated the need for column chromatography. The Schiff base ligand (HL=(E)-N-((6-(thiophen-2-yl)pyridin-2-yl)methylene)-1H-benzo[d]imidazol-2-amine)) was synthesized by the reaction of 6-(thiophen-2-yl)pyridine-2-carbaldehyde with 1H-benzimidazole-2-amine. Subsequently, metal(II) complexes of Co(II), Ni(II), and Cu(II) were prepared using this ligand. Structural analysis of both the ligand and its metal complexes was carried out using various physicochemical and spectroscopic methods. Ni(II) and Co(II) complexes were found to adopt an octahedral geometry, while the Cu(II) complex exhibited a square-planar structure. Binding studies with calf thymus DNA (CT-DNA) at pH 7.2 were performed using UV-visible spectroscopy, viscosity measurements, and thermal denaturation studies and showed that the metal complexes intercalate into the DNA and produced a distinct binding pattern. Molecular docking simulations with AutoDock Vina provided insights into the interaction of these complexes with the B-DNA dodecamer. Furthermore, the ligand and its metal complexes showed UV-visible photonuclease activity against pUC19 DNA. Agarose gel electrophoresis showed that the metal complexes exhibit photoinduced nuclease activity, confirming their ability to cleave DNA upon exposure to light.

本文介绍了一种利用低成本、可重复使用的铝酸钙纳米磷催化剂(CaAl2O4)合成含苯并咪唑基席夫碱化合物的新方法。这种方法避免了有害的溶剂和反应物,支持更环保的合成过程。催化剂在四个循环中保持了活性和非均质性,效率损失最小。合成过程简单,无需柱层析。以6-(噻吩-2-基)吡啶-2-基)亚甲基为原料,与h -苯并咪唑-2-胺反应合成了希夫碱配体(HL=(E)- n-((6-(噻吩-2-基)吡啶-2-基)亚甲基- 1h -苯并咪唑-2-胺)。随后,利用该配体制备了Co(II)、Ni(II)和Cu(II)的金属(II)配合物。利用各种物理化学和光谱方法对配体及其金属配合物进行了结构分析。Ni(II)和Co(II)配合物呈八面体结构,Cu(II)配合物呈方形平面结构。利用紫外可见光谱、粘度测量和热变性研究,在pH值为7.2时对小牛胸腺DNA (CT-DNA)进行了结合研究,结果表明金属配合物嵌入DNA并产生了独特的结合模式。利用AutoDock Vina进行分子对接模拟,可以深入了解这些复合物与B-DNA十二聚体的相互作用。此外,该配体及其金属配合物对pUC19 DNA具有紫外可见光酶活性。琼脂糖凝胶电泳显示,金属配合物表现出光诱导核酸酶活性,证实了它们在暴露于光下切割DNA的能力。
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引用次数: 0
Evaluation of ACE I/D and ATIR A1166C variants in patients with diabetes mellitus with and without peripheral neuropathy in Turkish patients. 土耳其伴有和不伴有周围神经病变的糖尿病患者中ACE I/D和ATIR A1166C变异的评估
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-17 DOI: 10.1080/15257770.2025.2451382
Payam Amiri Dashatan, Huseyin Soylu, Mehmet Elbistan, Aysegul Atmaca, Adem Keskin, Zulfinaz Betul Celik, Serbulent Yigit

Objective: Type 2 Diabetes Mellitus (T2DM) can lead to long-term vascular complications such as diabetic peripheral neuropathy (DPN). This study aimed to investigate the role of angiotensin-converting enzyme (ACE) insertion (I)/deletion (D) and angiotensin II type 1 receptor (AT1R) A1166C variants in the predisposition to T2DM in the Turkish population and their association with DPN.

Methods: The study included 90 T2DM patients (42 with DPN) and 50 healthy individuals. ACE I/D and ATIR A1166C gene regions were analyzed for the variant. Both the general genotype distribution of these variants and the observed genotype ratios were examined separately.

Results: In the T2DM group, the proportion of individuals with the AA genotype of the AT1R A1166C variant was lower than in the control group, and the proportion of individuals with the AC genotype was higher. There was no significant difference in the genotype distribution between the groups for the ACE I/D variant. There was no significant difference in the genotype distribution of the ACE I/D and ATIR A1166C variants in patients with and without DPN.

Conclusion: In the Turkish population, no significant difference was observed in the overall genotype distribution of ACE I/D and AT1R A1166C variants between T2DM patients and healthy individuals, whereas the AC genotype of the AT1R A1166C variant was more frequent in T2DM patients, and the AA genotype was less frequent. For both variants, no significant difference was observed in the genotype distribution between T2DM patients with and without DPN.

目的:2型糖尿病(T2DM)可导致糖尿病周围神经病变(DPN)等长期血管并发症。本研究旨在探讨血管紧张素转换酶(ACE)插入(I)/缺失(D)和血管紧张素II型1受体(AT1R) A1166C变异在土耳其人群中T2DM易感性中的作用及其与DPN的关系。方法:选取T2DM患者90例(合并DPN 42例)和健康人50例。分析了ACE I/D和ATIR A1166C基因区域的变异。对这些变异的一般基因型分布和观察到的基因型比率分别进行了检验。结果:T2DM组AT1R A1166C变异AA基因型个体比例低于对照组,AC基因型个体比例高于对照组。ACE I/D变异的基因型分布在两组间无显著差异。在有DPN和没有DPN的患者中,ACE I/D和ATIR A1166C变异的基因型分布无显著差异。结论:在土耳其人群中,T2DM患者与健康人群之间ACE I/D和AT1R A1166C变异的总体基因型分布无显著差异,而AT1R A1166C变异的AC基因型在T2DM患者中较多出现,AA基因型较少出现。对于这两种变异,合并和不合并DPN的T2DM患者的基因型分布无显著差异。
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引用次数: 0
Innovations in RNA therapeutics: a review of recent advances and emerging technologies. RNA疗法的创新:最新进展和新兴技术综述。
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-13 DOI: 10.1080/15257770.2025.2451377
Tuward J Dweh, Glay Jr Eric Wulu, John Kessellie Jallah, Dominic L Miller, Jyoti Prakash Sahoo

The field of biomedical science has witnessed another milestone with the advent of RNA-based therapeutics. This review explores three major RNA molecules, namely: messenger RNA (mRNA), RNA interference technology (RNAi), and Antisense Oligonucleotide (ASO), and analyses U.S. Food and Drug Administration drugs from 14 RNA-based pharmaceutical companies in terms of targeted genes, diseases and types, clinical trials and status, the mode of delivery, and the year of production. Many of such drugs are clinically approved or pending approval by the U.S. Food and Drug Administration (FDA) alongside other leading drugs agencies. Regarding diseases, this article emphasizes cancer therapy, genetic diseases, viral infections, and two categories of drug delivery systems include viral vectors and nanoparticles. Despite the tremendous progress made, key issues associated with these delivery systems are stability, off-target activities of RNA payloads, efficiency in cellular uptake, and the innovative need for engineering techniques for modifications. This review emphasizes the transformative potential of RNA therapeutics and the role of innovative technologies in addressing clinical needs, paving the way for a new era in precision medicine.

随着rna疗法的出现,生物医学科学领域见证了另一个里程碑。本文综述了信使RNA (mRNA)、RNA干扰技术(RNAi)和反义寡核苷酸(ASO)这三种主要RNA分子,并从靶向基因、疾病和类型、临床试验和现状、给药方式和生产年份等方面分析了美国食品和药物管理局(fda) 14家基于RNA的制药公司的药物。许多此类药物已获得美国食品和药物管理局(FDA)以及其他主要药物机构的临床批准或等待批准。在疾病方面,本文重点介绍了癌症治疗、遗传病、病毒感染,以及病毒载体和纳米颗粒两类药物传递系统。尽管取得了巨大的进展,但与这些传递系统相关的关键问题是稳定性、RNA有效载荷的脱靶活性、细胞摄取的效率以及对工程修饰技术的创新需求。这篇综述强调了RNA疗法的变革潜力和创新技术在解决临床需求方面的作用,为精准医学的新时代铺平了道路。
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引用次数: 0
Desalting of oligonucleotides through precipitation for mass spectrometric analysis. 通过沉淀法脱盐寡核苷酸用于质谱分析。
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-13 DOI: 10.1080/15257770.2025.2452387
Raissa Sultana, Eline Darjinoff, Hamza Qureshi, Liqun Qiu, Jonathon Roepke, Hongbin Yan

Contamination of sodium ions in oligonucleotides often causes issues in mass spectrometric analysis. This study investigated the efficiency of the combination of ammonium acetate and alcohol in desalting oligonucleotides. It was found that oligonucleotide samples containing up to 4 M NaCl can be effectively desalted through precipitation with ethanol or isopropanol in the presence of 1 or 5 M ammonium acetate. The level of sodium ions was reduced by 2-3 orders of magnitude as determined by atomic emission spectroscopy. The desalted samples were successfully analyzed by direct-infusion electrospray ionization mass spectrometry.

在质谱分析中,寡核苷酸中钠离子的污染经常引起问题。本研究考察了乙酸铵与醇联合脱盐寡核苷酸的效果。结果表明,在1 M或5 M乙酸铵存在下,乙醇或异丙醇沉淀法可以有效地脱盐4 M NaCl的寡核苷酸样品。通过原子发射光谱测定,钠离子的水平降低了2-3个数量级。采用直接输注电喷雾电离质谱法对脱盐样品进行了分析。
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引用次数: 0
Knockdown of miR-182 changes the sensitivity of triple-negative breast cancer cells to cisplatin. miR-182的敲低改变三阴性乳腺癌细胞对顺铂的敏感性。
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-11 DOI: 10.1080/15257770.2025.2451818
Hülya Dönmez, Bahadır Batar, Burhan Turgut

Breast cancer is the most common malignancy that affects women. MicroRNAs (miRNAs) play an essential role in cancer therapy and regulate many biological processes such as cisplatin resistance. The study's objective was to determine whether miR-182 dysregulation was the cause of cisplatin resistance in TNBC cell line MDA-MB-231. To determine the expression of miR-182, PCR was performed with primers specific to miR-182, and agarose gel electrophoresis was performed. To reduce the expression of miR-182 in MDA-MB-231 cells, anti-miR-182 oligonucleotides were used. RT-qPCR was used to confirm knockdown. The knockdown and control groups were treated with cisplatin at the same time. Propidium iodide (PI) and Annexin V staining were performed for apoptosis assay. Flow cytometric analysis was used to investigate the effect of miR-182 knockdown on cell cycle arrest. In comparison to untreated control MDA-MB-231 cells with MDA-MB-231 cells treated with anti-miR-182, there was a significant increase in the cisplatin-induced early apoptosis phase (p = 0.023). Also, inhibition of miR-182 significantly increased the cell cycle arrest at the G2/M phase in MDA-MB-231 cells (p = 0.031). Our results revealed that miR-182 inhibition may play a role in the overcoming of cisplatin resistance by inducing apoptosis and, cell cycle arrest in TNBC.

乳腺癌是女性最常见的恶性肿瘤。微RNA(miRNA)在癌症治疗中起着至关重要的作用,它调控着许多生物过程,如顺铂耐药性。本研究的目的是确定 miR-182 失调是否是 TNBC 细胞系 MDA-MB-231 产生顺铂耐药性的原因。为了确定miR-182的表达,研究人员使用miR-182的特异性引物进行了PCR,并进行了琼脂糖凝胶电泳。为了降低 miR-182 在 MDA-MB-231 细胞中的表达,使用了抗 miR-182 的寡核苷酸。使用 RT-qPCR 确认基因敲除。敲除组和对照组同时接受顺铂处理。碘化丙啶(PI)和Annexin V染色用于细胞凋亡检测。流式细胞分析用于研究 miR-182 敲除对细胞周期停滞的影响。与未处理的对照组 MDA-MB-231 细胞和抗 miR-182 处理的 MDA-MB-231 细胞相比,顺铂诱导的早期细胞凋亡阶段显著增加(p = 0.023)。此外,抑制 miR-182 能显著增加 MDA-MB-231 细胞在 G2/M 期的细胞周期停滞(p = 0.031)。我们的研究结果表明,抑制 miR-182 可通过诱导 TNBC 细胞凋亡和细胞周期停滞来克服顺铂耐药性。
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引用次数: 0
Taguchi method for optimization of Cr(VI) removal, isotherm, kinetic and thermodynamic studies. 田口法优化六价铬的去除、等温线、动力学和热力学研究。
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-01 Epub Date: 2024-02-06 DOI: 10.1080/15257770.2024.2308517
Sabrina Aziri, Smail Meziane, Hakima Bozetine, Nabila Berkane

In this study, Taguchi optimization method was applied to determine the optimum operating conditions for batch adsorption of Cr(VI) from aqueous solution. Initial pH of solution, adsorbent dose, initial hexavalent chromium concentration, contact time and adsorbent type were selected as the variables, and the removal efficiency of Cr(VI) was chosen for the designated response. L18(35) orthogonal array, signal-to-noise (S/N) ratio and analysis of variance statistical procedures were applied to identify the effect of each operating parameter on the removal of Cr(VI) from aqueous solution. The signal-to-noise (S/N) ratio results showed that the optimal combination for Cr(VI) removal was at pH 1.0, adsorbent dose of 3.6 g.L-1, Cr(VI) concentration of 30 mg.L-1, contact time of 95 min and olive leaves as adsorbent type. A removal of 95.09% was obtained at these optimum conditions. The analysis of variance of the data revealed that initial pH of solution was the most dominant parameter affecting Cr(VI) removal efficiency, followed by adsorbent type, adsorbent dose, contact time and initial metal concentration. Under optimal conditions, adsorption kinetic of Cr(VI) was studied and modeled using the pseudo first-order, pseudo-second-order and intraparticle diffusion models. It was found that the pseudo-second-order model fitted the adsorption data most with the highest determination coefficient (R2 = 0.996). Freundlich isotherm model, with regression coefficient R2 of 0.953, fit well with the equilibrium isotherm data. The Langmuir maximum adsorption capacity was found to be 62.5 mg.g-1. The experimental values of ΔH°, ΔG° and ΔS° revealed that the adsorption process was spontaneous and endothermic.

本研究采用田口优化法确定批量吸附水溶液中六价铬的最佳操作条件。选择溶液初始 pH 值、吸附剂剂量、六价铬初始浓度、接触时间和吸附剂类型作为变量,并选择六价铬的去除率作为指定响应。采用 L18(35)正交阵列、信噪比(S/N)和方差分析统计程序来确定各操作参数对水溶液中六价铬去除率的影响。信噪比(S/N)结果表明,pH 值为 1.0、吸附剂剂量为 3.6 g.L-1、六价铬浓度为 30 mg.L-1、接触时间为 95 分钟、吸附剂类型为橄榄叶时,六价铬去除率最佳。在这些最佳条件下,去除率达到 95.09%。数据的方差分析显示,溶液的初始 pH 值是影响六价铬去除率的最主要参数,其次是吸附剂类型、吸附剂剂量、接触时间和初始金属浓度。在最佳条件下,使用伪一阶、伪二阶和颗粒内扩散模型对 Cr(VI) 的吸附动力学进行了研究和建模。结果发现,伪一阶、伪二阶模型最符合吸附数据,确定系数最高(R2 = 0.996)。Freundlich 等温线模型的回归系数 R2 为 0.953,与平衡等温线数据非常吻合。朗缪尔最大吸附容量为 62.5 mg.g-1。ΔH°、ΔG°和ΔS°的实验值表明,吸附过程是自发的、内热的。
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引用次数: 0
The role of miRNA134 in pathogenesis and treatment of intractable epilepsy: a review article. miRNA134 在难治性癫痫发病机制和治疗中的作用:综述文章。
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-01 Epub Date: 2024-03-26 DOI: 10.1080/15257770.2024.2331046
Maniya Kasaiyan, Mohsen Basiri, Sara Pajouhanfar

MicroRNA-134 (miRNA134) has emerged as a critical regulator in the pathogenesis of epilepsy, particularly in intractable cases resistant to conventional therapies. This review explores the multifaceted roles of miRNA134 in epileptogenesis, focusing on its influence on dendritic spine morphology and synaptic plasticity. Through its interactions with proteins such as LIM kinase 1 (LIMK1), Pumilio 2 (PUM2), and Tubby-like protein 1 (TULP1), miRNA134 modulates various molecular pathways implicated in epilepsy development. Preclinical studies have shown pro-mising results in targeting miRNA134 for mitigating seizure activity, highlighting its potential as a therapeutic target. Furthermore, miRNA134 holds promise as a biomarker for epilepsy diagnosis and prognosis, offering opportunities for personalized treatment approaches. However, further research is warranted to elucidate the precise mechanisms underlying miRNA134's effects and to translate these findings into clinical applications.

microRNA-134(miRNA134)已成为癫痫发病机制中的一个关键调节因子,尤其是在对传统疗法耐药的难治性病例中。本综述探讨了 miRNA134 在癫痫发生过程中的多方面作用,重点关注其对树突棘形态和突触可塑性的影响。通过与 LIM 激酶 1 (LIMK1)、Pumilio 2 (PUM2) 和 Tubby-like 蛋白 1 (TULP1) 等蛋白的相互作用,miRNA134 可调节与癫痫发生有关的各种分子通路。临床前研究表明,以 miRNA134 为靶点可减轻癫痫发作活动,这突显了 miRNA134 作为治疗靶点的潜力。此外,miRNA134 有希望成为癫痫诊断和预后的生物标志物,为个性化治疗方法提供机会。然而,要阐明 miRNA134 作用的确切机制并将这些发现转化为临床应用,还需要进一步的研究。
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引用次数: 0
Prognostic value and immunological role of MMRN1: a rising star in cancer. MMRN1 的预后价值和免疫学作用:癌症中的一颗新星。
IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-01 Epub Date: 2024-05-07 DOI: 10.1080/15257770.2024.2335680
Qing Zhou, Ying Liu, Jieyu Zhou, Wenling Zhang

Background: Multimerin 1 (MMRN1) is a factor V binding protein, which can support platelet adhesion and thrombus formation. In recent years, the role of MMRN1 in cancer has begun to attract attention. But systematic studies in this area are lacking. Therefore, we used bioinformatics methods to analyze MMRN1 in tumors to reveal the possible role of MMRN1.

Methods: Using the Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) database, we obtained relevant data for analyzing MMRN1. Using Gene Expression Profiling Interactive Analysis (GEPIA), Human Protein Atlas (HPA), TCGA, GeneMANIA, and cBioPortal, we explored the potential role of MMRN1 in different types of tumors. Tumor Immune System Interactions and Drug Bank (TISIDB) and Sangerbox were used to analyze the correlation between MMRN1 and tumor immunity. Gene set cancer analysis (GSCA) and UALCAN were used to analyze the methylation of MMRN1. GSCA was also used to analyze the drug sensitivity of MMRN1.

Results: MMRN1 is down-regulated in most cancer types and is closely related to the prognosis of cancer patients. Interestingly, in most tumors, MMRN1 is positively correlated with immune -related genes. In addition, we observed different levels of methylation and mutations in different types of tumors. Drug sensitivity analysis found that MMRN1 was negatively correlated with several drugs, including GW-2580 and TL-1-85, suggesting that it can be used to develop potential anticancer therapies.

Conclusion: Our analysis demonstrated a significant relationship between MMRN1 and prognosis, tumor immunity, and drug sensitivity of several tumors. As a rising star in cancer, it needs further research.

背景:多聚蛋白1(MMRN1)是一种因子V结合蛋白,可支持血小板粘附和血栓形成。近年来,MMRN1在癌症中的作用开始受到关注。但目前还缺乏这方面的系统研究。因此,我们利用生物信息学方法对肿瘤中的 MMRN1 进行了分析,以揭示 MMRN1 的可能作用:利用癌症基因组图谱(TCGA)和基因型-组织表达(GTEx)数据库,我们获得了分析 MMRN1 的相关数据。利用基因表达谱交互分析(GEPIA)、人类蛋白质图谱(HPA)、TCGA、GeneMANIA和cBioPortal,我们探索了MMRN1在不同类型肿瘤中的潜在作用。肿瘤免疫系统相互作用与药物库(TISIDB)和Sangerbox用于分析MMRN1与肿瘤免疫之间的相关性。基因组癌症分析(GSCA)和 UALCAN 用于分析 MMRN1 的甲基化情况。GSCA还用于分析MMRN1的药物敏感性:结果:MMRN1在大多数癌症类型中下调,并与癌症患者的预后密切相关。有趣的是,在大多数肿瘤中,MMRN1与免疫相关基因呈正相关。此外,我们还在不同类型的肿瘤中观察到不同程度的甲基化和突变。药物敏感性分析发现,MMRN1与包括GW-2580和TL-1-85在内的几种药物呈负相关,这表明它可用于开发潜在的抗癌疗法:我们的分析表明,MMRN1与多种肿瘤的预后、肿瘤免疫力和药物敏感性之间存在重要关系。作为癌症领域的后起之秀,它还需要进一步研究。
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引用次数: 0
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