Evaluation of bi-layer silk fibroin grafts for onlay urethroplasty in a rabbit model of urethral stricture disease.

IF 2.4 4区医学 Q4 CELL & TISSUE ENGINEERING Regenerative medicine Pub Date : 2024-08-30 DOI:10.1080/17460751.2024.2389753

Gokhan Gundogdu, Jay Budrewicz, Jodie Giordano, Raffaele Melidone, Chris Searcy, Vikas Agarwal, Carlos R Estrada, Joshua R Mauney

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Abstract

Background: Autologous tissues such as buccal mucosa (BM) are widely used for reconstruction of urethral strictures; however, limitations such as donor site morbidity and scarce tissue supply require the development of alternative biomaterials for urethral repair. The goals of this study were to determine the safety and efficacy of bi-layer silk fibroin (BLSF) matrices for urethral stricture repair and compare histological and functional outcomes to the standard approach, BM urethroplasty under good laboratory practices.Material and methods: A total of 13 rabbits exhibiting urethral stricture formation following electrocoagulation injury were treated with onlay urethroplasty with either acellular BLSF (N = 7) or autologous BM (N = 6) grafts for 3 months. Uninjured control rabbits were maintained in parallel (N = 4).Results and conclusion: Animals receiving BLSF implants were demonstrated to be functionally equivalent to BM grafts in their ability to restored strictured calibers, support micturition and promote tissue regeneration with minimal inflammation.

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在兔尿道狭窄疾病模型中评估双层丝纤维蛋白移植物用于尿道嵌顿成形术的效果。

背景：颊粘膜（BM）等自体组织被广泛用于尿道狭窄的重建；然而，由于供体部位发病率和组织供应稀缺等限制，需要开发用于尿道修复的替代生物材料。本研究的目的是确定双层蚕丝纤维蛋白（BLSF）基质用于尿道狭窄修复的安全性和有效性，并将组织学和功能结果与标准方法--良好实验室规范下的 BM 尿道成形术--进行比较：共对 13 只电凝损伤后尿道狭窄的兔子进行了为期 3 个月的无细胞 BLSF（7 只）或自体 BM（6 只）移植物尿道成形术。同时饲养未受伤的对照组兔子（4 只）：结果和结论：接受 BLSF 植入物的动物在功能上与 BM 移植物相当，它们能够恢复狭窄的管径、支持排尿并促进组织再生，同时炎症极少。

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来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.