Identification of cross-reactive allergens between the Dermatophagoides farinae house dust mite and the Toxocara canis nematode in dogs with suspected allergies.

IF 1.9 3区 农林科学 Q3 DERMATOLOGY Veterinary dermatology Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI:10.1111/vde.13295
Thierry Olivry, Ana Mas-Fontao, Sandrine Jacquenet, Martina Aumayr, Toshihiro Tsukui, Véronique Gomord, Loïc Faye, Claude Favrot
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Abstract

Background: Immunoglobulin (Ig)E cross-reactivity has been shown between Dermatophagoides farinae (Df; house dust mite) and the nematode Toxocara canis (Tc), yet its allergen basis is unknown.

Objectives: To identify the Df allergens IgE-cross-reactive with those of Tc.

Animals: Archived sera from 73 dogs with suspected allergy sensitised to Df.

Materials and methods: We performed a combination of Pet Allergy Xplorer (PAX) and enzyme-linked immunosorbent assay (ELISA) inhibitions with excretory-secretory and somatic (i.e. nematode body) extracts of Tc or recombinant Tc tropomyosin on coats of Df, Der f 15 and Zen-1 (ELISA) or PAX allergens.

Results: The ELISA and PAX inhibitions established that there is mutual yet variable cross-reactivity between the Tc excretory-secretory extract, purified Der f 15 and purified Zen-1. This cross-reactivity is likely to involve cross-reactive glycans, as there is no inhibition between the Tc excretory-secretory extract and recombinant Der f 15 without its predicted natural O-glycans. We also confirmed a heterogeneous cross-reactivity between the somatic Tc extract and Der p 11 (paramyosin), as well as between the recombinant Toxo c 3 and Der p 10 tropomyosins. The cross-reactivity among tropomyosins and paramyosins is likely to involve peptidic epitopes, as these recombinant allergens are not glycosylated.

Conclusions and clinical relevance: In dogs with suspected allergies, the cross-reactivity between Tc and Df for dogs is complex and heterogeneous. Some of the cross-reactive IgE recognises shared glycans on Der f 15 and Zen-1, while some targets peptidic epitopes on shared paramyosins and tropomyosins. We do not exclude that additional cross-reactive allergens between Df and Tc also might exist.

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在疑似过敏的狗身上鉴定 Dermatophagoides farinae 屋尘螨和犬弓形虫线虫之间的交叉反应过敏原。
背景:Dermatophagoides farinae(Df;家尘螨)与犬线虫(Tc)之间已出现免疫球蛋白(Ig)E交叉反应,但其过敏原基础尚不清楚:目的:确定与 Tc 的 IgE 交叉反应的 Df 过敏原:动物:73 只疑似对 Df 过敏的狗的存档血清:我们在Df、Der f 15和Zen-1(酶联免疫吸附试验)或PAX过敏原的皮毛上用Tc或重组Tc肌球蛋白的排泄-分泌和体细胞(即线虫体)提取物进行了宠物过敏Xplorer(PAX)和酶联免疫吸附试验(ELISA)组合抑制试验:结果:ELISA 和 PAX 抑制试验表明,锝排泄-分泌提取物、纯化的 Der f 15 和纯化的 Zen-1 之间存在相互但不同的交叉反应。这种交叉反应可能涉及交叉反应糖,因为锝排泄-分泌提取物与不含天然 O 型糖的重组 Der f 15 之间没有抑制作用。我们还证实了体细胞 Tc 提取物与 Der p 11(副肌球蛋白)之间以及重组毒素 c 3 与 Der p 10 肌球蛋白之间的异质性交叉反应。肌球蛋白和副肌球蛋白之间的交叉反应可能涉及肽表位,因为这些重组过敏原没有糖基化:在疑似过敏的犬中,Tc 和 Df 之间的交叉反应是复杂而多样的。一些交叉反应 IgE 识别 Der f 15 和 Zen-1 上的共有糖基,而另一些则针对共有的副肌球蛋白和肌球蛋白上的肽表位。我们不排除 Df 和 Tc 之间还可能存在其他交叉反应过敏原。
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来源期刊
Veterinary dermatology
Veterinary dermatology 农林科学-兽医学
CiteScore
3.20
自引率
21.40%
发文量
92
审稿时长
12-24 weeks
期刊介绍: Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities
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